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The map label for this gene is 13472781

Identifier: 13472781

GI number: 13472781

Start: 2551516

End: 2552934

Strand: Direct

Name: 13472781

Synonym: mlr3187

Alternate gene names: NA

Gene position: 2551516-2552934 (Clockwise)

Preceding gene: 13472780

Following gene: 13472782

Centisome position: 36.26

GC content: 67.09

Gene sequence:

>1419_bases
GTGCCGCGGGCCGGCGATGTCTGCCGCCTGTCCGCCGTCGAGCTGGCCGGCGCCATTCGCCGCAAGAAACTGTCCGTGCG
CGAGGTGGTGACGGCCTTCCTCGACCGCATCGAAACGGTCAACCCGCTGGTCAACGCCATCGTCTCGTTGCGCGAGCGCG
GCGATATCCTGCGCGAGGCCGATGCAGCCGATGCCAGGCAAGCGAGTGAAACCGGGAAGCTCTTCGGCCTGCCGATCGCC
ATCAAGGACCTCGCCTCGACGACCGGGCTCAGGACCTCCTTCGGCTCGCCGATCTTCGCCGATTTCGTGCCGCAGGAAGA
CGATTTCTTCGTCGAGCGCATCCGCAACGCCGGCGCCATCATCATCGGCAAGACCAATGTCCCCGAATTCGGGTTGGGAT
CCAACACCTACAACAATGTCTTCGGGCCGACGCTGAACGCTTTCGATCCGGCGCTCACCGCCGGCGGCTCGAGCGGCGGC
GCGGCGGTGGCGCTGGCGCTCGACATGGTCCCCGTCGCCGATGGCAGCGACTTCGGCGGCTCGCTGCGCAATCCGGCAGG
CTGGAACAATGTCTACGGTTTCCGGCCGTCGCAAGGGCTTGTTCCCGCCGGACCCGATCTTGAGGTCTTCCATGCGCAGA
TGGGCATCGAGGGTCCGATGGGCCGCAATGTCGCCGACATGGCGCTGCTGCTCGACGTACAGGCGGGCTACCATCCGCAG
GCGCCGCTGTCCTATGAGAAACCGGGCTCGTTCCTCGAAGGGCTTGCACCGCCCTCGACCGGCGGCCGCGTCGCCTGGCT
CGGCGACCTCGGCGGCCATCTGCCGATCGAAGCGGGCATTCTCGATCTGTGCCAGGCGGCACTCGGCCGGTTCTCGGACC
AGTCCTTCGCGGTTGAACCCTTGCTCCCGGATTTCGACTTCGAAGCGCTCTGGCAAGCCTTCGTGACGCTGCGCCAGGCC
AGCAGCGGCTGCGCCCTCAAGGTCCATTACGACGACCCTTCCAGGCGTGGCATGCTGAAGCCGGAAGCCATATGGGAAAT
CGAGAACGCCATGCGGCTGACGGCGCCGCAGATCCGCGCGGCGTCGGTCGTGCGCACCTCCTGGCATCGGATGCTGCTGT
CGATCTTCGACCGCTTCGACCTCATAGCCCTGCCCACCGCGCAGGTCTTCCCCTTCGAAGTCGGCATGCACTGGCCAAGC
GAGGTCGCTGGACGGCCGATGGACAGCTATCACCGCTGGATGCAGGTTTCAGCCTTCGCCACGCTTGGCGGCTGCCCGGC
GGTCAACGTACCGGTCGGCTTCGACGACAAGGGCCGGCCAATGGGCATGCAGCTGATCGGCCGGCCGCGTGGCGACCTCG
CGGTGCTACGGGCGGCGACCGCCTATGAGGCGACACTGCCCTGGCAGGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
CTGAAGCCTGGCGCCCAGCCGGCGATCCCGCTGGCCAAGGATGTCGATCAAGCCTTGCAGGAAGTCAGCAGAAATGATGC
GCCCTGAGACCAAGCATGCC

Downstream 100 bases:

>100_bases
CGCGCGCGCAACGGCGCGGTGTTTCGCGTCTGGCTTGCGCAGAGGCTACCACCATGCATTGATTGTCCCCTGCCACGCCG
CATTGGCGGGACTCCAAGGG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPRAGDVCRLSAVELAGAIRRKKLSVREVVTAFLDRIETVNPLVNAIVSLRERGDILREADAADARQASETGKLFGLPIA
IKDLASTTGLRTSFGSPIFADFVPQEDDFFVERIRNAGAIIIGKTNVPEFGLGSNTYNNVFGPTLNAFDPALTAGGSSGG
AAVALALDMVPVADGSDFGGSLRNPAGWNNVYGFRPSQGLVPAGPDLEVFHAQMGIEGPMGRNVADMALLLDVQAGYHPQ
APLSYEKPGSFLEGLAPPSTGGRVAWLGDLGGHLPIEAGILDLCQAALGRFSDQSFAVEPLLPDFDFEALWQAFVTLRQA
SSGCALKVHYDDPSRRGMLKPEAIWEIENAMRLTAPQIRAASVVRTSWHRMLLSIFDRFDLIALPTAQVFPFEVGMHWPS
EVAGRPMDSYHRWMQVSAFATLGGCPAVNVPVGFDDKGRPMGMQLIGRPRGDLAVLRAATAYEATLPWQAGA

Sequences:

>Translated_472_residues
MPRAGDVCRLSAVELAGAIRRKKLSVREVVTAFLDRIETVNPLVNAIVSLRERGDILREADAADARQASETGKLFGLPIA
IKDLASTTGLRTSFGSPIFADFVPQEDDFFVERIRNAGAIIIGKTNVPEFGLGSNTYNNVFGPTLNAFDPALTAGGSSGG
AAVALALDMVPVADGSDFGGSLRNPAGWNNVYGFRPSQGLVPAGPDLEVFHAQMGIEGPMGRNVADMALLLDVQAGYHPQ
APLSYEKPGSFLEGLAPPSTGGRVAWLGDLGGHLPIEAGILDLCQAALGRFSDQSFAVEPLLPDFDFEALWQAFVTLRQA
SSGCALKVHYDDPSRRGMLKPEAIWEIENAMRLTAPQIRAASVVRTSWHRMLLSIFDRFDLIALPTAQVFPFEVGMHWPS
EVAGRPMDSYHRWMQVSAFATLGGCPAVNVPVGFDDKGRPMGMQLIGRPRGDLAVLRAATAYEATLPWQAGA
>Mature_471_residues
PRAGDVCRLSAVELAGAIRRKKLSVREVVTAFLDRIETVNPLVNAIVSLRERGDILREADAADARQASETGKLFGLPIAI
KDLASTTGLRTSFGSPIFADFVPQEDDFFVERIRNAGAIIIGKTNVPEFGLGSNTYNNVFGPTLNAFDPALTAGGSSGGA
AVALALDMVPVADGSDFGGSLRNPAGWNNVYGFRPSQGLVPAGPDLEVFHAQMGIEGPMGRNVADMALLLDVQAGYHPQA
PLSYEKPGSFLEGLAPPSTGGRVAWLGDLGGHLPIEAGILDLCQAALGRFSDQSFAVEPLLPDFDFEALWQAFVTLRQAS
SGCALKVHYDDPSRRGMLKPEAIWEIENAMRLTAPQIRAASVVRTSWHRMLLSIFDRFDLIALPTAQVFPFEVGMHWPSE
VAGRPMDSYHRWMQVSAFATLGGCPAVNVPVGFDDKGRPMGMQLIGRPRGDLAVLRAATAYEATLPWQAGA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=243, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=24.9027237354086, Blast_Score=109, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=25.6565656565657, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=455, Percent_Identity=28.3516483516484, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=284, Percent_Identity=26.056338028169, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=252, Percent_Identity=29.3650793650794, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=268, Percent_Identity=26.865671641791, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=28.2850779510022, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320448, Length=225, Percent_Identity=31.1111111111111, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=140, Percent_Identity=39.2857142857143, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648435, Length=501, Percent_Identity=26.5469061876248, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648437, Length=501, Percent_Identity=26.5469061876248, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45550774, Length=501, Percent_Identity=26.5469061876248, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652985, Length=242, Percent_Identity=35.1239669421488, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19922090, Length=242, Percent_Identity=35.1239669421488, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24652981, Length=242, Percent_Identity=35.1239669421488, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24652983, Length=242, Percent_Identity=35.1239669421488, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=25.9405940594059, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=245, Percent_Identity=32.2448979591837, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356731, Length=233, Percent_Identity=32.618025751073, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648441, Length=425, Percent_Identity=24.9411764705882, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648439, Length=425, Percent_Identity=24.9411764705882, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648113, Length=169, Percent_Identity=37.8698224852071, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 50614; Mature: 50483

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRAGDVCRLSAVELAGAIRRKKLSVREVVTAFLDRIETVNPLVNAIVSLRERGDILREA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAADARQASETGKLFGLPIAIKDLASTTGLRTSFGSPIFADFVPQEDDFFVERIRNAGAI
CHHHHHHHHHCCCEEECCHHHHHHHHHCCCCHHCCCCHHHHCCCCCCHHHHHHHHCCCEE
IIGKTNVPEFGLGSNTYNNVFGPTLNAFDPALTAGGSSGGAAVALALDMVPVADGSDFGG
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEEECCCCCCCCC
SLRNPAGWNNVYGFRPSQGLVPAGPDLEVFHAQMGIEGPMGRNVADMALLLDVQAGYHPQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCCCC
APLSYEKPGSFLEGLAPPSTGGRVAWLGDLGGHLPIEAGILDLCQAALGRFSDQSFAVEP
CCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECC
LLPDFDFEALWQAFVTLRQASSGCALKVHYDDPSRRGMLKPEAIWEIENAMRLTAPQIRA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
ASVVRTSWHRMLLSIFDRFDLIALPTAQVFPFEVGMHWPSEVAGRPMDSYHRWMQVSAFA
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHHH
TLGGCPAVNVPVGFDDKGRPMGMQLIGRPRGDLAVLRAATAYEATLPWQAGA
HHCCCCEECCCCCCCCCCCCCCHHEECCCCCCEEHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
PRAGDVCRLSAVELAGAIRRKKLSVREVVTAFLDRIETVNPLVNAIVSLRERGDILREA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAADARQASETGKLFGLPIAIKDLASTTGLRTSFGSPIFADFVPQEDDFFVERIRNAGAI
CHHHHHHHHHCCCEEECCHHHHHHHHHCCCCHHCCCCHHHHCCCCCCHHHHHHHHCCCEE
IIGKTNVPEFGLGSNTYNNVFGPTLNAFDPALTAGGSSGGAAVALALDMVPVADGSDFGG
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEEECCCCCCCCC
SLRNPAGWNNVYGFRPSQGLVPAGPDLEVFHAQMGIEGPMGRNVADMALLLDVQAGYHPQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCCCC
APLSYEKPGSFLEGLAPPSTGGRVAWLGDLGGHLPIEAGILDLCQAALGRFSDQSFAVEP
CCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECC
LLPDFDFEALWQAFVTLRQASSGCALKVHYDDPSRRGMLKPEAIWEIENAMRLTAPQIRA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
ASVVRTSWHRMLLSIFDRFDLIALPTAQVFPFEVGMHWPSEVAGRPMDSYHRWMQVSAFA
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHHH
TLGGCPAVNVPVGFDDKGRPMGMQLIGRPRGDLAVLRAATAYEATLPWQAGA
HHCCCCEECCCCCCCCCCCCCCHHEECCCCCCEEHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]