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The map label for this gene is paaF [C]

Identifier: 13472678

GI number: 13472678

Start: 2453697

End: 2454473

Strand: Direct

Name: paaF [C]

Synonym: mlr3048

Alternate gene names: 13472678

Gene position: 2453697-2454473 (Clockwise)

Preceding gene: 13472677

Following gene: 13472679

Centisome position: 34.87

GC content: 67.82

Gene sequence:

>777_bases
ATGGCTGAGCCTCTCGTCACCTTCGAAAGCGAGGGCGCCATCGGCATTGTCACCTTGCGCCGCCCGGAAAAGTTCAACGC
GCTGGACATCCCGATGCTGCGCGCGCTCGAAGCCGCGCTCGACGAGGCGGAGCTGGCCGAAGGCGTACGCGCCGTGCTCT
TGCGCGGCGAAGGCAAGGGCTTCTGCGCCGGTGGCGATGTCGAGGCCTGGGGCGCAATGAGCGCTGCCGACTTCCAGGTG
CAATGGGTGCGCTACGGCCACCGCGTCTTCGACCGGCTGGCGCGGTTGCGGCAGCCGACCATCGCCGTGCTGTCCGGCCA
TGCACTGGGCGGCGGGCTGGAATTAGCCGTCGCCTGCGATTTCCGGGTCGCCGAGGCGCATGTGAAATTGGGCTTCCCCG
AAACCTCGATCGGCGTCGTGCCGGGCTGGTCCGGCACGCAGCGCGCGGTGCGCCGCTTCGGTGCGCAGACCGTGCGGCGC
ATGGCGTTGGGTGGCGAGGTTTTTCTGGCTGCCGACGCGCTGGCTTTGGCTATCGTTGACCGCGTTGTCGAGACCGGCAG
GGCGTTCGACGAAGCCAAGGCCTGGGGGGAAAAAATAGCCGAGCGCGGCCCGCTGGCGACGGAAGCGGCCAAGCTGATGA
TCGCGGTGGCCGAGGGCGAGGAAAGTGCGGCCGCTACCGAAGCGCTGGCCAGCGGCTTTATCGCGCTCACCGGCGACCTC
AAGGCCGGTGTCGACGCCTTCAAGGCCAAGCAGATGCCGGTATTCTCAAGAAGCTAG

Upstream 100 bases:

>100_bases
CTCGGTGTCGCCAACGACCTCAAGGTGACGCCGGCGGCGCTCTACCAGGATCGGCCGATCGGCCTGTCGCTCAATGGCGG
CGCCTCGCTCGGAGGCGCGC

Downstream 100 bases:

>100_bases
AGAAACAGTCCCGATGAACGCACCCCTCAACATCGCCATGCCCGCCGAGGCCTCGGCAGCGCCGAAAAATTACCGGCTGC
TGATCGACGGCAAGCATGTC

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAEPLVTFESEGAIGIVTLRRPEKFNALDIPMLRALEAALDEAELAEGVRAVLLRGEGKGFCAGGDVEAWGAMSAADFQV
QWVRYGHRVFDRLARLRQPTIAVLSGHALGGGLELAVACDFRVAEAHVKLGFPETSIGVVPGWSGTQRAVRRFGAQTVRR
MALGGEVFLAADALALAIVDRVVETGRAFDEAKAWGEKIAERGPLATEAAKLMIAVAEGEESAAATEALASGFIALTGDL
KAGVDAFKAKQMPVFSRS

Sequences:

>Translated_258_residues
MAEPLVTFESEGAIGIVTLRRPEKFNALDIPMLRALEAALDEAELAEGVRAVLLRGEGKGFCAGGDVEAWGAMSAADFQV
QWVRYGHRVFDRLARLRQPTIAVLSGHALGGGLELAVACDFRVAEAHVKLGFPETSIGVVPGWSGTQRAVRRFGAQTVRR
MALGGEVFLAADALALAIVDRVVETGRAFDEAKAWGEKIAERGPLATEAAKLMIAVAEGEESAAATEALASGFIALTGDL
KAGVDAFKAKQMPVFSRS
>Mature_257_residues
AEPLVTFESEGAIGIVTLRRPEKFNALDIPMLRALEAALDEAELAEGVRAVLLRGEGKGFCAGGDVEAWGAMSAADFQVQ
WVRYGHRVFDRLARLRQPTIAVLSGHALGGGLELAVACDFRVAEAHVKLGFPETSIGVVPGWSGTQRAVRRFGAQTVRRM
ALGGEVFLAADALALAIVDRVVETGRAFDEAKAWGEKIAERGPLATEAAKLMIAVAEGEESAAATEALASGFIALTGDLK
AGVDAFKAKQMPVFSRS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=30.9803921568627, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=31.25, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI213417737, Length=207, Percent_Identity=27.536231884058, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI157694516, Length=207, Percent_Identity=27.536231884058, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=32.03125, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=29.5081967213115, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI157694520, Length=183, Percent_Identity=26.775956284153, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594469, Length=143, Percent_Identity=31.4685314685315, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI37594471, Length=143, Percent_Identity=31.4685314685315, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70995211, Length=249, Percent_Identity=26.5060240963855, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=36.8421052631579, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=23.0769230769231, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=31.2741312741313, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790281, Length=186, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788682, Length=176, Percent_Identity=36.3636363636364, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI221142681, Length=208, Percent_Identity=31.25, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=23.9543726235741, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=221, Percent_Identity=35.2941176470588, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=29.2307692307692, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=30.8641975308642, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=220, Percent_Identity=30.9090909090909, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=220, Percent_Identity=30.9090909090909, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=220, Percent_Identity=30.9090909090909, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17558304, Length=235, Percent_Identity=27.6595744680851, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=24.3137254901961, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=211, Percent_Identity=27.9620853080569, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=30.9278350515464, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=30.9278350515464, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=26.7605633802817, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=196, Percent_Identity=27.5510204081633, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=202, Percent_Identity=30.6930693069307, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=196, Percent_Identity=27.5510204081633, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=31.0756972111554, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=31.0756972111554, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=31.3492063492063, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571729, Length=177, Percent_Identity=28.8135593220339, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571730, Length=177, Percent_Identity=28.8135593220339, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=30.1980198019802, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=35.0574712643678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=35.0574712643678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=35.0574712643678, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=188, Percent_Identity=27.1276595744681, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583165, Length=223, Percent_Identity=26.457399103139, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921018, Length=241, Percent_Identity=25.7261410788382, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27278; Mature: 27146

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPLVTFESEGAIGIVTLRRPEKFNALDIPMLRALEAALDEAELAEGVRAVLLRGEGKG
CCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCE
FCAGGDVEAWGAMSAADFQVQWVRYGHRVFDRLARLRQPTIAVLSGHALGGGLELAVACD
EECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEC
FRVAEAHVKLGFPETSIGVVPGWSGTQRAVRRFGAQTVRRMALGGEVFLAADALALAIVD
EEEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
RVVETGRAFDEAKAWGEKIAERGPLATEAAKLMIAVAEGEESAAATEALASGFIALTGDL
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHEEEEEEECCCCHHHHHHHHHCCEEEEECCH
KAGVDAFKAKQMPVFSRS
HHCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEPLVTFESEGAIGIVTLRRPEKFNALDIPMLRALEAALDEAELAEGVRAVLLRGEGKG
CCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCE
FCAGGDVEAWGAMSAADFQVQWVRYGHRVFDRLARLRQPTIAVLSGHALGGGLELAVACD
EECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEC
FRVAEAHVKLGFPETSIGVVPGWSGTQRAVRRFGAQTVRRMALGGEVFLAADALALAIVD
EEEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
RVVETGRAFDEAKAWGEKIAERGPLATEAAKLMIAVAEGEESAAATEALASGFIALTGDL
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHEEEEEEECCCCHHHHHHHHHCCEEEEECCH
KAGVDAFKAKQMPVFSRS
HHCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA