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The map label for this gene is puuB [H]

Identifier: 13472653

GI number: 13472653

Start: 2423579

End: 2424790

Strand: Direct

Name: puuB [H]

Synonym: mlr3020

Alternate gene names: 13472653

Gene position: 2423579-2424790 (Clockwise)

Preceding gene: 13472652

Following gene: 13472654

Centisome position: 34.45

GC content: 66.91

Gene sequence:

>1212_bases
ATGCCCCGCAAACTCGACCTCAGAACTGGCCGGCCCGTCTGGTCCGCCTATCGGGCGCCAGCCGTGCCGACTTCGGCGCT
GACCCGGGACGTCACGACTGATGTGCTGATCATCGGCATGGGGATCAGCGGCGCCATGATGGCCGAGGCATTGACGGCGG
ATGGCCATTCCGTGATCTGCATCGACCGGCGCGGGCCGCTGAAGGGCTCGACCGCGGCGACCACCGCGCTGGTCCAGTTC
GAAATCGACCAGCCGCTTTCAACCCTCTCGAAGATGATCGGCAAGGCCAAGGCGCAGCAGGCCTGGCGCCGCTCGCGCCT
CGCCATTTCCAACCTGGCCGGCCGCATCGAGGATCTCGCAATCGACTGTCGCCTGCGTCGCACCCCGTCGCTTTATCTGG
CCGGCACGCTGCTCGGCCCGGCGGAAATGCGCGATGAAGCCGAAGCGCGGCGACAGGCGGGCGTTGCCGCAACCTATCTG
ACGCCGCAGCCGCTGGCGCAGACATTCGGCATCGACCGCGGCGGCGCCATCCTCAGCCACGGCAACATCGCGCTCGACCC
GCGCAAGCTGACGGCCGGGCTGCTGCTCAAGGCGCTGGAGCGCAAGGCACGTCTTTACGCTCCCGCCGAAGCGACGGCGA
TCGAGGATAGCGCCGGCGAGGCGGTCGTGGCCACGAAGGATGGCCCGACCATCACCGCACGGCATGTGGTTCTGGCCACC
GGCTATGAACTGGTCGACATCGTGCCGGCGGCCGCCCACCGGATCATCTCGACCTGGGCGATCGCGACGTGCCCGCAACC
GCGGAAACTCTGGCCGGGTCCGGCTTTCATCTGGGAGGCGTCGGACCCCTACCTTTACCTCAGGGCGACGGCGGACGGCA
GGGTCATCTGCGGCGGCGAGGACGAGGATTTTGCCGACGAGACGCGACGCGACGAACTGATCGCGGACAAGAGTGCCCGC
ATTGCCGACAAGCTGGGCCGGCTGTTCCCCTATCTCGACGTGAGGCCCGAATTTGCCTGGACCGGTTCCTTCGGCACAAC
CAGCGCCGGCCTGCCCTATATCGGCGCCGTCCCGGGGCACCCCCGCATCCACGCCGTCATGGGGTACGGCGGCAATGGCA
TCACCTTCTCACGTATCGCCTCCGAAATTATTTCGGCCTCGATCAAAGGGAGTGCCGACACAGATGCAGAGCTCTTCGCC
TTCAACCGTTGA

Upstream 100 bases:

>100_bases
CAACCAAAGAGTCGTCAACCAAAGAGTTAGTGCGGCTCGCCGCTCTGACACCGGAACAACGGCCACTCCGGGGCGTTGAG
GCGCACTTCCGGAGAATTCG

Downstream 100 bases:

>100_bases
TTGCAAATCGACAAACAATCCTTCATCACCTGATTGTAACGCTCCTGAAACACACTTAAGTCTTTGGTGAAGTCGGCCGC
GGGGGAGTGGCTCTCGCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPRKLDLRTGRPVWSAYRAPAVPTSALTRDVTTDVLIIGMGISGAMMAEALTADGHSVICIDRRGPLKGSTAATTALVQF
EIDQPLSTLSKMIGKAKAQQAWRRSRLAISNLAGRIEDLAIDCRLRRTPSLYLAGTLLGPAEMRDEAEARRQAGVAATYL
TPQPLAQTFGIDRGGAILSHGNIALDPRKLTAGLLLKALERKARLYAPAEATAIEDSAGEAVVATKDGPTITARHVVLAT
GYELVDIVPAAAHRIISTWAIATCPQPRKLWPGPAFIWEASDPYLYLRATADGRVICGGEDEDFADETRRDELIADKSAR
IADKLGRLFPYLDVRPEFAWTGSFGTTSAGLPYIGAVPGHPRIHAVMGYGGNGITFSRIASEIISASIKGSADTDAELFA
FNR

Sequences:

>Translated_403_residues
MPRKLDLRTGRPVWSAYRAPAVPTSALTRDVTTDVLIIGMGISGAMMAEALTADGHSVICIDRRGPLKGSTAATTALVQF
EIDQPLSTLSKMIGKAKAQQAWRRSRLAISNLAGRIEDLAIDCRLRRTPSLYLAGTLLGPAEMRDEAEARRQAGVAATYL
TPQPLAQTFGIDRGGAILSHGNIALDPRKLTAGLLLKALERKARLYAPAEATAIEDSAGEAVVATKDGPTITARHVVLAT
GYELVDIVPAAAHRIISTWAIATCPQPRKLWPGPAFIWEASDPYLYLRATADGRVICGGEDEDFADETRRDELIADKSAR
IADKLGRLFPYLDVRPEFAWTGSFGTTSAGLPYIGAVPGHPRIHAVMGYGGNGITFSRIASEIISASIKGSADTDAELFA
FNR
>Mature_402_residues
PRKLDLRTGRPVWSAYRAPAVPTSALTRDVTTDVLIIGMGISGAMMAEALTADGHSVICIDRRGPLKGSTAATTALVQFE
IDQPLSTLSKMIGKAKAQQAWRRSRLAISNLAGRIEDLAIDCRLRRTPSLYLAGTLLGPAEMRDEAEARRQAGVAATYLT
PQPLAQTFGIDRGGAILSHGNIALDPRKLTAGLLLKALERKARLYAPAEATAIEDSAGEAVVATKDGPTITARHVVLATG
YELVDIVPAAAHRIISTWAIATCPQPRKLWPGPAFIWEASDPYLYLRATADGRVICGGEDEDFADETRRDELIADKSARI
ADKLGRLFPYLDVRPEFAWTGSFGTTSAGLPYIGAVPGHPRIHAVMGYGGNGITFSRIASEIISASIKGSADTDAELFAF
NR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=388, Percent_Identity=24.4845360824742, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 43174; Mature: 43043

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKLDLRTGRPVWSAYRAPAVPTSALTRDVTTDVLIIGMGISGAMMAEALTADGHSVIC
CCCEECCCCCCCHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEEE
IDRRGPLKGSTAATTALVQFEIDQPLSTLSKMIGKAKAQQAWRRSRLAISNLAGRIEDLA
EECCCCCCCCCHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IDCRLRRTPSLYLAGTLLGPAEMRDEAEARRQAGVAATYLTPQPLAQTFGIDRGGAILSH
EEEEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCEEEEC
GNIALDPRKLTAGLLLKALERKARLYAPAEATAIEDSAGEAVVATKDGPTITARHVVLAT
CCEEECHHHHHHHHHHHHHHHHHHEECCCHHCEECCCCCCEEEEECCCCEEEEEEEEEEC
GYELVDIVPAAAHRIISTWAIATCPQPRKLWPGPAFIWEASDPYLYLRATADGRVICGGE
CCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEECCC
DEDFADETRRDELIADKSARIADKLGRLFPYLDVRPEFAWTGSFGTTSAGLPYIGAVPGH
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCEEECCCCC
PRIHAVMGYGGNGITFSRIASEIISASIKGSADTDAELFAFNR
CCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECC
>Mature Secondary Structure 
PRKLDLRTGRPVWSAYRAPAVPTSALTRDVTTDVLIIGMGISGAMMAEALTADGHSVIC
CCEECCCCCCCHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEEE
IDRRGPLKGSTAATTALVQFEIDQPLSTLSKMIGKAKAQQAWRRSRLAISNLAGRIEDLA
EECCCCCCCCCHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IDCRLRRTPSLYLAGTLLGPAEMRDEAEARRQAGVAATYLTPQPLAQTFGIDRGGAILSH
EEEEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCEEEEC
GNIALDPRKLTAGLLLKALERKARLYAPAEATAIEDSAGEAVVATKDGPTITARHVVLAT
CCEEECHHHHHHHHHHHHHHHHHHEECCCHHCEECCCCCCEEEEECCCCEEEEEEEEEEC
GYELVDIVPAAAHRIISTWAIATCPQPRKLWPGPAFIWEASDPYLYLRATADGRVICGGE
CCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEECCC
DEDFADETRRDELIADKSARIADKLGRLFPYLDVRPEFAWTGSFGTTSAGLPYIGAVPGH
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCEEECCCCC
PRIHAVMGYGGNGITFSRIASEIISASIKGSADTDAELFAFNR
CCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]