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The map label for this gene is gcvA [H]

Identifier: 13472549

GI number: 13472549

Start: 2319533

End: 2320444

Strand: Direct

Name: gcvA [H]

Synonym: mlr2880

Alternate gene names: 13472549

Gene position: 2319533-2320444 (Clockwise)

Preceding gene: 13472538

Following gene: 13472558

Centisome position: 32.97

GC content: 67.0

Gene sequence:

>912_bases
ATGAGTGTCCAGCGCCGCCTTTTGCCCAACATCAGCGCCCTCGCCGCTTTCGAGGCGGTGGCCCGGCTTGGGTCCTTCAC
GGCTGCCGCGCAGGAGCTGGACCTGACCCAGGGTGCTGTCAGCCGGCAGATCCGACTGCTCGAAGACCAGTTCGGGCGCC
GGCTGTTCGAGCGCGACAGCCGCAATGTCCGGCTGTCGACGGCAGGCGAAATCTACGCCGAAGCGGTGCGTTCAGCTCTC
GGCCAGTTGCGCGACGCAGCCCTTGGACTGATGAGCAATCGGCATAGTGGCATCTTGAACCTCGCCATTTTGCCGACCTT
CGGCACCCGCTGGCTGATGCCGATGATCCCGGATTTCGTCGCGAACAATCCCGACATCACCATCAACTTCGTCACCCGCG
TCGGCCGCTTCGACTTTGCCAGGGAAAGACTGGACGCCGCCATCCACTACGGCCTGCCGGACTGGCCGGAAGCCGATTCG
ACGCTGCTGATGAGCGAAACCGTGGCGCCTGTGGTGTCGCCCGAGTTCCTCGCCAGCCGCGCCATCGCCACGCCGGCCGA
TATCGGCCGCCAGCCGCTGCTGCACATGGCGACGCGCCCCGGCGCCTGGACCGAATGGTTCGAGCATCAGGGCCTGAGCG
CGCCGGCCGGACCCGGCATGCAGTTCGAACAATTCGGCACCGCCGCCCAGGCCTGCATGGCGGGGCTTGGCGTCGCGCTG
TTGCCGCTTCTCCTCATCGCGGGCGAATTGCAGCGCGGCCAGTTGGTGCCGGCGCCCGGCCAGCCCATGCAAAGCCGCAG
CGCCTATTACCTCGTCGTCCCGCACGACAAGCGCGAACATCCCCCGGTGGCCAGCTTTCGCGACTGGCTGCTGGAGCAGG
TCGAGAAGGAGCCGGCTGTTCTGGCGTGGTAG

Upstream 100 bases:

>100_bases
CAATCGGTCCTCAAACGGGATGAAGCCCGGCTTATCGGCCCGCGGCCTAAAAAAATCAAACGAAATTGCTTCACGGATCA
ATGAGCGTTAGGAATAGATC

Downstream 100 bases:

>100_bases
CTACTTGCGCTTCAGCGCATCGGCCAGCGCCGCACCGAAGGCCCCTTGCTGGGCCGGCCGTTCCGGCTGGCGTTGCGGCG
CTGGCGAGCGCGGCGCCGGC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSVQRRLLPNISALAAFEAVARLGSFTAAAQELDLTQGAVSRQIRLLEDQFGRRLFERDSRNVRLSTAGEIYAEAVRSAL
GQLRDAALGLMSNRHSGILNLAILPTFGTRWLMPMIPDFVANNPDITINFVTRVGRFDFARERLDAAIHYGLPDWPEADS
TLLMSETVAPVVSPEFLASRAIATPADIGRQPLLHMATRPGAWTEWFEHQGLSAPAGPGMQFEQFGTAAQACMAGLGVAL
LPLLLIAGELQRGQLVPAPGQPMQSRSAYYLVVPHDKREHPPVASFRDWLLEQVEKEPAVLAW

Sequences:

>Translated_303_residues
MSVQRRLLPNISALAAFEAVARLGSFTAAAQELDLTQGAVSRQIRLLEDQFGRRLFERDSRNVRLSTAGEIYAEAVRSAL
GQLRDAALGLMSNRHSGILNLAILPTFGTRWLMPMIPDFVANNPDITINFVTRVGRFDFARERLDAAIHYGLPDWPEADS
TLLMSETVAPVVSPEFLASRAIATPADIGRQPLLHMATRPGAWTEWFEHQGLSAPAGPGMQFEQFGTAAQACMAGLGVAL
LPLLLIAGELQRGQLVPAPGQPMQSRSAYYLVVPHDKREHPPVASFRDWLLEQVEKEPAVLAW
>Mature_302_residues
SVQRRLLPNISALAAFEAVARLGSFTAAAQELDLTQGAVSRQIRLLEDQFGRRLFERDSRNVRLSTAGEIYAEAVRSALG
QLRDAALGLMSNRHSGILNLAILPTFGTRWLMPMIPDFVANNPDITINFVTRVGRFDFARERLDAAIHYGLPDWPEADST
LLMSETVAPVVSPEFLASRAIATPADIGRQPLLHMATRPGAWTEWFEHQGLSAPAGPGMQFEQFGTAAQACMAGLGVALL
PLLLIAGELQRGQLVPAPGQPMQSRSAYYLVVPHDKREHPPVASFRDWLLEQVEKEPAVLAW

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=33.9041095890411, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=32.404181184669, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788706, Length=304, Percent_Identity=31.5789473684211, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=25.5102040816327, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=26.8456375838926, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=35.8024691358025, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=28.9036544850498, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=26.510067114094, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=24.7422680412371, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=256, Percent_Identity=26.171875, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367136, Length=203, Percent_Identity=28.0788177339901, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33272; Mature: 33140

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQRRLLPNISALAAFEAVARLGSFTAAAQELDLTQGAVSRQIRLLEDQFGRRLFERDS
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RNVRLSTAGEIYAEAVRSALGQLRDAALGLMSNRHSGILNLAILPTFGTRWLMPMIPDFV
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
ANNPDITINFVTRVGRFDFARERLDAAIHYGLPDWPEADSTLLMSETVAPVVSPEFLASR
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHH
AIATPADIGRQPLLHMATRPGAWTEWFEHQGLSAPAGPGMQFEQFGTAAQACMAGLGVAL
HCCCCHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LPLLLIAGELQRGQLVPAPGQPMQSRSAYYLVVPHDKREHPPVASFRDWLLEQVEKEPAV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCE
LAW
ECC
>Mature Secondary Structure 
SVQRRLLPNISALAAFEAVARLGSFTAAAQELDLTQGAVSRQIRLLEDQFGRRLFERDS
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RNVRLSTAGEIYAEAVRSALGQLRDAALGLMSNRHSGILNLAILPTFGTRWLMPMIPDFV
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
ANNPDITINFVTRVGRFDFARERLDAAIHYGLPDWPEADSTLLMSETVAPVVSPEFLASR
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHH
AIATPADIGRQPLLHMATRPGAWTEWFEHQGLSAPAGPGMQFEQFGTAAQACMAGLGVAL
HCCCCHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LPLLLIAGELQRGQLVPAPGQPMQSRSAYYLVVPHDKREHPPVASFRDWLLEQVEKEPAV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCE
LAW
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]