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The map label for this gene is trmL [H]

Identifier: 13472427

GI number: 13472427

Start: 2184860

End: 2185318

Strand: Direct

Name: trmL [H]

Synonym: mlr2718

Alternate gene names: 13472427

Gene position: 2184860-2185318 (Clockwise)

Preceding gene: 13472422

Following gene: 13472428

Centisome position: 31.05

GC content: 65.8

Gene sequence:

>459_bases
ATGAACCGTCGTCTCCGCATCGCGCTCTACCAACCGGACATTGCCGGCAATACCGGGACAATCCTGCGCTTCGCCGCTTG
TCTCGGCCTTGGCGTCGACATCATCGAGCCGGCCGGCTTTCCGCTTTCCGACAAGGCGCTCAAGCGCGCCGGTATGGACT
ACCTCGAAATGGCTGCCCTGACCCGGCATGTCGACTGGAACGCCTTCGAGGACCGGCGCAAGAAGGACGGGCGCCGGCTG
GTGCTTCTGTCGACCAAGGCTGCGACCCCCTACACCAACTTCAGCTTTGCCGACGGCGACATCCTGCTGTTCGGCCGCGA
ATCCGCGGGCGTGCCGGATCCTGTCCATCAGGCGGCGGATGCGCGGCTGACCATCCCCATGCAGGGCAGCGCGCGCAGCA
TCAATGTCGCGCTGTCCGTCGCCATGGTGGCGGGCGAAGCCATCCGCCAGCTCGGATAG

Upstream 100 bases:

>100_bases
CATACCCGACTTCGCAGAAAATGGCCACCCGCAGGTCCAGGAATTTCCAAAACCGGGAAGCCCCTGTGGCCGATCTGCCA
CTTATTAGAAGGATGCGGAA

Downstream 100 bases:

>100_bases
GATCGGCGGTCCATCGATGGCCTCGCCGTCGCCGACGACAGCGGTTTCTCCGGTCATTCTCACCCAGGCGCACATCGCCT
TCTCCTGTCATTTGCCCAAT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MNRRLRIALYQPDIAGNTGTILRFAACLGLGVDIIEPAGFPLSDKALKRAGMDYLEMAALTRHVDWNAFEDRRKKDGRRL
VLLSTKAATPYTNFSFADGDILLFGRESAGVPDPVHQAADARLTIPMQGSARSINVALSVAMVAGEAIRQLG

Sequences:

>Translated_152_residues
MNRRLRIALYQPDIAGNTGTILRFAACLGLGVDIIEPAGFPLSDKALKRAGMDYLEMAALTRHVDWNAFEDRRKKDGRRL
VLLSTKAATPYTNFSFADGDILLFGRESAGVPDPVHQAADARLTIPMQGSARSINVALSVAMVAGEAIRQLG
>Mature_152_residues
MNRRLRIALYQPDIAGNTGTILRFAACLGLGVDIIEPAGFPLSDKALKRAGMDYLEMAALTRHVDWNAFEDRRKKDGRRL
VLLSTKAATPYTNFSFADGDILLFGRESAGVPDPVHQAADARLTIPMQGSARSINVALSVAMVAGEAIRQLG

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=150, Percent_Identity=44, Blast_Score=124, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16470; Mature: 16470

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRLRIALYQPDIAGNTGTILRFAACLGLGVDIIEPAGFPLSDKALKRAGMDYLEMAAL
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHCCCHHHHHHHH
TRHVDWNAFEDRRKKDGRRLVLLSTKAATPYTNFSFADGDILLFGRESAGVPDPVHQAAD
HHCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCCHHHHCCC
ARLTIPMQGSARSINVALSVAMVAGEAIRQLG
CEEEEEECCCCCEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRRLRIALYQPDIAGNTGTILRFAACLGLGVDIIEPAGFPLSDKALKRAGMDYLEMAAL
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHCCCHHHHHHHH
TRHVDWNAFEDRRKKDGRRLVLLSTKAATPYTNFSFADGDILLFGRESAGVPDPVHQAAD
HHCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCCHHHHCCC
ARLTIPMQGSARSINVALSVAMVAGEAIRQLG
CEEEEEECCCCCEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA