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The map label for this gene is neo [H]

Identifier: 13472409

GI number: 13472409

Start: 2168653

End: 2169444

Strand: Direct

Name: neo [H]

Synonym: mlr2696

Alternate gene names: 13472409

Gene position: 2168653-2169444 (Clockwise)

Preceding gene: 13472408

Following gene: 13472410

Centisome position: 30.82

GC content: 65.53

Gene sequence:

>792_bases
ATGTCCAGCCGTCCTGCGGAAACCGGCCTGCCGAACGGGTTGCGCCAGTTGGTTTCCGGCTATCGCTGGCATCGGCAGAC
CATCGGCCAGTCACAGGCCGGCGTGTTCCGGCTGGCAGCCGACGGCAGGCCGGCGCTGTTCCTGAAATGCGAGCGCAGCG
GTCCCTTTGCCGAACTCGCCGATGAAGCCGCACGGCTGCGCTGGCTTACCGGACAAGGCGTTGCTTGCCCCGATGTGATC
GCCCTGGAAAGCCATGCCGGCTACGACTGGCTGCTGATGTCGGCCGTCGCCGGCGAGGATCTGGCGTCGGCGGCGATCGA
TCAGGCCGATGTCATCGGCATCATGGCGAGCGCGCTTCGCGATCTGCATGCGATCGACATCCGCTCCTGTCCCTTCGACC
ATCGCCTCATCAGGCGGATCGCGGCGGCGCGGGCCCGTATGGAAGGCGGCCAAGTCGACGAAAGCGATTTCGACGAGGAA
CGGCAAGGGCGGACCGACGCCGAGGTCTTCGCCGAGCTTGAGGCACTCCGGCCGGCGACCGAGGATCTTGTGGTCACCCA
TGGCGACGCCTGCCTGCCGAATGTCATGACTGCGGGGGGCGGTTTCAGTGGTTTCATCGATTGCGGCCGACTGGGCGTGG
CGGATCGCCACCAGGATCTGGCGCTCGCCTGCTGGAGCATCCGCTACAATCTCGGCCAGGTCTGGATAGAGCCTTTTCTC
AAGCGGTACGAGCCGCCGGAAACCGAGCCGGCGAAGCTTTCCTGGTATCGGCTGCTCGACGAGTTCTTCTGA

Upstream 100 bases:

>100_bases
GGAAGCCGGCAAGGCGCGCGACGAAGGCAAGGAATATGTCGTCCAGGACGGCGACATCATGCTGTTCAAGTTCAACACCT
GACGCTCCAAGCAGCAGACG

Downstream 100 bases:

>100_bases
GCGGCCGATCCCGGCCCGGCTCCCTGGGCTCGTCTTGATTTTGTCACCTCACGGGTCTAAGCGGCTGGCACACGGTCGCG
CACACGACGAAGAGCCGTGC

Product: kanamycin resistance protein

Products: NA

Alternate protein names: APH(3')-II; APH(3')II; Kanamycin kinase, type II; Neomycin-kanamycin phosphotransferase type II [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSSRPAETGLPNGLRQLVSGYRWHRQTIGQSQAGVFRLAADGRPALFLKCERSGPFAELADEAARLRWLTGQGVACPDVI
ALESHAGYDWLLMSAVAGEDLASAAIDQADVIGIMASALRDLHAIDIRSCPFDHRLIRRIAAARARMEGGQVDESDFDEE
RQGRTDAEVFAELEALRPATEDLVVTHGDACLPNVMTAGGGFSGFIDCGRLGVADRHQDLALACWSIRYNLGQVWIEPFL
KRYEPPETEPAKLSWYRLLDEFF

Sequences:

>Translated_263_residues
MSSRPAETGLPNGLRQLVSGYRWHRQTIGQSQAGVFRLAADGRPALFLKCERSGPFAELADEAARLRWLTGQGVACPDVI
ALESHAGYDWLLMSAVAGEDLASAAIDQADVIGIMASALRDLHAIDIRSCPFDHRLIRRIAAARARMEGGQVDESDFDEE
RQGRTDAEVFAELEALRPATEDLVVTHGDACLPNVMTAGGGFSGFIDCGRLGVADRHQDLALACWSIRYNLGQVWIEPFL
KRYEPPETEPAKLSWYRLLDEFF
>Mature_262_residues
SSRPAETGLPNGLRQLVSGYRWHRQTIGQSQAGVFRLAADGRPALFLKCERSGPFAELADEAARLRWLTGQGVACPDVIA
LESHAGYDWLLMSAVAGEDLASAAIDQADVIGIMASALRDLHAIDIRSCPFDHRLIRRIAAARARMEGGQVDESDFDEER
QGRTDAEVFAELEALRPATEDLVVTHGDACLPNVMTAGGGFSGFIDCGRLGVADRHQDLALACWSIRYNLGQVWIEPFLK
RYEPPETEPAKLSWYRLLDEFF

Specific function: Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.95 [H]

Molecular weight: Translated: 28988; Mature: 28857

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRPAETGLPNGLRQLVSGYRWHRQTIGQSQAGVFRLAADGRPALFLKCERSGPFAELA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCHHHHH
DEAARLRWLTGQGVACPDVIALESHAGYDWLLMSAVAGEDLASAAIDQADVIGIMASALR
HHHHHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
DLHAIDIRSCPFDHRLIRRIAAARARMEGGQVDESDFDEERQGRTDAEVFAELEALRPAT
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCC
EDLVVTHGDACLPNVMTAGGGFSGFIDCGRLGVADRHQDLALACWSIRYNLGQVWIEPFL
CCEEEECCCCHHCHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KRYEPPETEPAKLSWYRLLDEFF
HHCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
SSRPAETGLPNGLRQLVSGYRWHRQTIGQSQAGVFRLAADGRPALFLKCERSGPFAELA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCHHHHH
DEAARLRWLTGQGVACPDVIALESHAGYDWLLMSAVAGEDLASAAIDQADVIGIMASALR
HHHHHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
DLHAIDIRSCPFDHRLIRRIAAARARMEGGQVDESDFDEERQGRTDAEVFAELEALRPAT
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCC
EDLVVTHGDACLPNVMTAGGGFSGFIDCGRLGVADRHQDLALACWSIRYNLGQVWIEPFL
CCEEEECCCCHHCHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KRYEPPETEPAKLSWYRLLDEFF
HHCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6295884; 3889831; 12628253 [H]