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The map label for this gene is prs

Identifier: 13472400

GI number: 13472400

Start: 2159627

End: 2160562

Strand: Direct

Name: prs

Synonym: mlr2685

Alternate gene names: 13472400

Gene position: 2159627-2160562 (Clockwise)

Preceding gene: 13472399

Following gene: 13472402

Centisome position: 30.69

GC content: 63.57

Gene sequence:

>936_bases
ATGAAACTCTTCGCGGGCAATTCCAACAGGGTGCTGGCCGAAGCGGTCGCCCGCTATCTCAACATCCCGCTGGGCAAGGC
CACTGTCAGGCGCTTCGCCGACCAGGAAATCTTCGTCGAAATCCAGGAAAACGTGCGCGGCGAGGATGTCTTCATCCTGC
AGTCGACCTCGTTTCCGACCAACGATCATTTGATGGAACTGCTCATCATGATCGACGCCTTCATGCGCTCCTCGGCCAAG
CGCATCACGGCGGTGATTCCCTATTTCGGCTATGCCAGGCAGGACCGCCGGGCGTCGGGCCGCACGCCGATCTCGGCCAA
GCTGGTCGCCAACATGATCACCCGCGCCGGCGTCGACCGCGTTCTGACGCTGGACCTGCATGCCGGCCAGATCCAGGGCT
TCTTCGACATCCCGACCGACAACCTGTTCTCGGTGCCGGTGATGGCCCGCGACGTGAAGGCGAAATACAAGCAGCTCGGC
AACGTCGTGGTGGTGTCGCCCGACATTGGCGGCGTGGTGCGGGCGCGGGCGCTTGCCAAGCGCTTCGACGCGCAGCTCGC
CATCGTCGACAAGCGCCGTGAGCGCCCGGGCGAATCGGAAGTCATGAACATTATCGGCGCGGTCGCCGGCAAGGACTGCC
TGCTGATCGACGACATCGTCGATTCCGGCGGCACGCTGTGCAATGCCGCCGATGCGCTCTTGGCCAACGGTGCCACCAGC
GTCACCGCCTATATCACCCATGGCGTGCTGTCAGGCGGCGCTGTGGCCCGTATCAGCGGCTCGAAACTGCAGGAACTGGT
GATCACCGATTCCATCCAGCCGACGCAAGGCGTGCTCGACGCCCCCAACATCCGCGTCATCTCGATCGCCGACCTGATGG
GCGAAGCGATCTCGCGCACGGCAACCGAGGAGTCGGTGTCGAGCCTGTTCGACTAA

Upstream 100 bases:

>100_bases
CGATCTCGGCGCTTGCAATACCAGCACGGGCCGCTAAAAGCCCGATTAAAAGGTACGGGAGAGTCTGTCAGGCCTTCCCA
TCCCCCACCAGGAACGGTGC

Downstream 100 bases:

>100_bases
ACCTCCGCTATCGACGAGACGTCACCCGGGCGGAGCGGACGCCCCTGTTCGCGCCTGCGGGCATCCCCGCGGACGTGATC
GAGGTTCTCCACAAGGCAGC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKLFAGNSNRVLAEAVARYLNIPLGKATVRRFADQEIFVEIQENVRGEDVFILQSTSFPTNDHLMELLIMIDAFMRSSAK
RITAVIPYFGYARQDRRASGRTPISAKLVANMITRAGVDRVLTLDLHAGQIQGFFDIPTDNLFSVPVMARDVKAKYKQLG
NVVVVSPDIGGVVRARALAKRFDAQLAIVDKRRERPGESEVMNIIGAVAGKDCLLIDDIVDSGGTLCNAADALLANGATS
VTAYITHGVLSGGAVARISGSKLQELVITDSIQPTQGVLDAPNIRVISIADLMGEAISRTATEESVSSLFD

Sequences:

>Translated_311_residues
MKLFAGNSNRVLAEAVARYLNIPLGKATVRRFADQEIFVEIQENVRGEDVFILQSTSFPTNDHLMELLIMIDAFMRSSAK
RITAVIPYFGYARQDRRASGRTPISAKLVANMITRAGVDRVLTLDLHAGQIQGFFDIPTDNLFSVPVMARDVKAKYKQLG
NVVVVSPDIGGVVRARALAKRFDAQLAIVDKRRERPGESEVMNIIGAVAGKDCLLIDDIVDSGGTLCNAADALLANGATS
VTAYITHGVLSGGAVARISGSKLQELVITDSIQPTQGVLDAPNIRVISIADLMGEAISRTATEESVSSLFD
>Mature_311_residues
MKLFAGNSNRVLAEAVARYLNIPLGKATVRRFADQEIFVEIQENVRGEDVFILQSTSFPTNDHLMELLIMIDAFMRSSAK
RITAVIPYFGYARQDRRASGRTPISAKLVANMITRAGVDRVLTLDLHAGQIQGFFDIPTDNLFSVPVMARDVKAKYKQLG
NVVVVSPDIGGVVRARALAKRFDAQLAIVDKRRERPGESEVMNIIGAVAGKDCLLIDDIVDSGGTLCNAADALLANGATS
VTAYITHGVLSGGAVARISGSKLQELVITDSIQPTQGVLDAPNIRVISIADLMGEAISRTATEESVSSLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=308, Percent_Identity=46.4285714285714, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI4506127, Length=310, Percent_Identity=46.1290322580645, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI28557709, Length=310, Percent_Identity=45.4838709677419, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI84875539, Length=311, Percent_Identity=45.9807073954984, Blast_Score=291, Evalue=5e-79,
Organism=Homo sapiens, GI4506133, Length=342, Percent_Identity=34.7953216374269, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=31.8713450292398, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI310128524, Length=141, Percent_Identity=32.6241134751773, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310115209, Length=141, Percent_Identity=32.6241134751773, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310118259, Length=141, Percent_Identity=32.6241134751773, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310119946, Length=141, Percent_Identity=32.6241134751773, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787458, Length=313, Percent_Identity=54.9520766773163, Blast_Score=343, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17554702, Length=310, Percent_Identity=44.8387096774194, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI25149168, Length=310, Percent_Identity=44.8387096774194, Blast_Score=287, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI71989924, Length=310, Percent_Identity=44.8387096774194, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=44.8051948051948, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17570245, Length=337, Percent_Identity=33.2344213649852, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319403, Length=312, Percent_Identity=45.1923076923077, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=44.8717948717949, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321776, Length=311, Percent_Identity=45.016077170418, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=40.8163265306122, Blast_Score=155, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=86, Percent_Identity=40.6976744186046, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=310, Percent_Identity=46.1290322580645, Blast_Score=289, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45551540, Length=333, Percent_Identity=42.9429429429429, Blast_Score=276, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24651458, Length=351, Percent_Identity=31.3390313390313, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651456, Length=351, Percent_Identity=31.3390313390313, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI281362873, Length=351, Percent_Identity=31.3390313390313, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651454, Length=351, Percent_Identity=31.3390313390313, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651462, Length=370, Percent_Identity=30.5405405405405, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651464, Length=370, Percent_Identity=30.5405405405405, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45552010, Length=370, Percent_Identity=30.5405405405405, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_RHILO (Q98HW3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103967.1
- ProteinModelPortal:   Q98HW3
- SMR:   Q98HW3
- GeneID:   1226628
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr2685
- NMPDR:   fig|266835.1.peg.2071
- HOGENOM:   HBG519284
- OMA:   YKTAGAD
- ProtClustDB:   PRK01259
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 33519; Mature: 33519

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFAGNSNRVLAEAVARYLNIPLGKATVRRFADQEIFVEIQENVRGEDVFILQSTSFPT
CEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHEEHHCCCCCEEEEEECCCCCC
NDHLMELLIMIDAFMRSSAKRITAVIPYFGYARQDRRASGRTPISAKLVANMITRAGVDR
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLTLDLHAGQIQGFFDIPTDNLFSVPVMARDVKAKYKQLGNVVVVSPDIGGVVRARALAK
EEEEEECCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
RFDAQLAIVDKRRERPGESEVMNIIGAVAGKDCLLIDDIVDSGGTLCNAADALLANGATS
HHCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHCCCCH
VTAYITHGVLSGGAVARISGSKLQELVITDSIQPTQGVLDAPNIRVISIADLMGEAISRT
HHHHHHHHHHCCCEEEEECCHHHHHHHEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHH
ATEESVSSLFD
HHHHHHHHHCC
>Mature Secondary Structure
MKLFAGNSNRVLAEAVARYLNIPLGKATVRRFADQEIFVEIQENVRGEDVFILQSTSFPT
CEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHEEHHCCCCCEEEEEECCCCCC
NDHLMELLIMIDAFMRSSAKRITAVIPYFGYARQDRRASGRTPISAKLVANMITRAGVDR
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLTLDLHAGQIQGFFDIPTDNLFSVPVMARDVKAKYKQLGNVVVVSPDIGGVVRARALAK
EEEEEECCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
RFDAQLAIVDKRRERPGESEVMNIIGAVAGKDCLLIDDIVDSGGTLCNAADALLANGATS
HHCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHCCCCH
VTAYITHGVLSGGAVARISGSKLQELVITDSIQPTQGVLDAPNIRVISIADLMGEAISRT
HHHHHHHHHHCCCEEEEECCHHHHHHHEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHH
ATEESVSSLFD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968