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The map label for this gene is dagK [H]

Identifier: 13472344

GI number: 13472344

Start: 2109394

End: 2110431

Strand: Reverse

Name: dagK [H]

Synonym: mll2607

Alternate gene names: 13472344

Gene position: 2110431-2109394 (Counterclockwise)

Preceding gene: 13472346

Following gene: 13472340

Centisome position: 29.99

GC content: 65.51

Gene sequence:

>1038_bases
ATGTTGCAATCGACGGCGCCCCTTGCTCCGCCGGCGGGCGCGGGCTTCAATGCCGGCAAACGATCGAGCGTTCATGCTCG
GCGCCGGGGAGGGTGCGTATTGAAAGTCGGGGTGGTTCTCAATCCGATAGCCGGTGGCGGCCGGCTGAAGCGGCATTGGC
CTGAAGTCGCCGCCTCACTGAAAAAGCACTTCGGCGATTTCGAACTGCGCGAGACGCAGGCGGAGGGCGACGCCGAACGG
TTGGCCATCGACCTCGCCGCCACCGGCTTCGACCTGGTGATCGCCGCCGGTGGCGACGGCACCGCCAGCGAAGTGGCCGA
CGGGCTGCTGCAGGCATTCGAGGAGAGCGGTAGGACGACCGAGCTTGGCCTGCTGCCATGCGGTACAGGCATCGATTTCG
CACGCGGTCTTGGCCTGCCGAAAGCCGTGGACGCGACCTTGAAGCGGATCGCCGGGGCAGAGGGCCGCAAGGTCGATGCC
GGCCGCATCTGCTATATCGACGACCATGGCGCACTGGCTAGCCGCCACTTCATCAACATCGCCAGCCTCGGCCTGTCGGG
CGCCACCGATCGCGCCGTCAATGCCGACAAGCGAAAGGGCAAGATGTCGGCCAAGGCGCTGTTCCTGTGGCGCACCGTGG
TCGAGTTCATCCGCTACCGCTTCCAGCATGTGCGGATCACCGTCGACGACGGCGCTCCGGTCGAGGCGCGCATGGCGCTG
GTGGCGGTGGCCAACGGCAAGTTCTTCGGCGGCGGCATGATGATCGCGCCGGATGCCGAGCTCACCGACGGGCAATTCGA
CATCGTCATCCTGCGCGCGGCAGGTAAGCTGAAGCTGATCTGGGACATCCGCCTGCTCTATGGCGGCCGGCACCGCAACC
ATCCGGCAATCACCATCCTGCGCGGCAAGAAAGTGGTTGTCGAACCGCTCGACGATGTGGAAAAGAACGGCGCGCTGGTG
GACATAGACGGTGAGTCGCCCGGGCGCATACCGGCGACCTTCGAGATCCTACCTGGCGCGCTGACGCTCAGATATTGA

Upstream 100 bases:

>100_bases
ATGGCCATGGGCCTGTGGATAGCGGGATTATCGGGGACCGTTTGGCGAGAGCCCAGGAACTTCAGTGCCCGCTGCCTTAG
ACAGCCTGCCCCTCTTTTCG

Downstream 100 bases:

>100_bases
ATCAAGGCGTTGCCAGATTGATTCAAGTCTTTTTGAAACTGAATCCGGCTATCGATTCAAGCAAATGAAATTGCTTGGTT
TTCCGGTCTCCGGTCAGATT

Product: hypothetical protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLQSTAPLAPPAGAGFNAGKRSSVHARRRGGCVLKVGVVLNPIAGGGRLKRHWPEVAASLKKHFGDFELRETQAEGDAER
LAIDLAATGFDLVIAAGGDGTASEVADGLLQAFEESGRTTELGLLPCGTGIDFARGLGLPKAVDATLKRIAGAEGRKVDA
GRICYIDDHGALASRHFINIASLGLSGATDRAVNADKRKGKMSAKALFLWRTVVEFIRYRFQHVRITVDDGAPVEARMAL
VAVANGKFFGGGMMIAPDAELTDGQFDIVILRAAGKLKLIWDIRLLYGGRHRNHPAITILRGKKVVVEPLDDVEKNGALV
DIDGESPGRIPATFEILPGALTLRY

Sequences:

>Translated_345_residues
MLQSTAPLAPPAGAGFNAGKRSSVHARRRGGCVLKVGVVLNPIAGGGRLKRHWPEVAASLKKHFGDFELRETQAEGDAER
LAIDLAATGFDLVIAAGGDGTASEVADGLLQAFEESGRTTELGLLPCGTGIDFARGLGLPKAVDATLKRIAGAEGRKVDA
GRICYIDDHGALASRHFINIASLGLSGATDRAVNADKRKGKMSAKALFLWRTVVEFIRYRFQHVRITVDDGAPVEARMAL
VAVANGKFFGGGMMIAPDAELTDGQFDIVILRAAGKLKLIWDIRLLYGGRHRNHPAITILRGKKVVVEPLDDVEKNGALV
DIDGESPGRIPATFEILPGALTLRY
>Mature_345_residues
MLQSTAPLAPPAGAGFNAGKRSSVHARRRGGCVLKVGVVLNPIAGGGRLKRHWPEVAASLKKHFGDFELRETQAEGDAER
LAIDLAATGFDLVIAAGGDGTASEVADGLLQAFEESGRTTELGLLPCGTGIDFARGLGLPKAVDATLKRIAGAEGRKVDA
GRICYIDDHGALASRHFINIASLGLSGATDRAVNADKRKGKMSAKALFLWRTVVEFIRYRFQHVRITVDDGAPVEARMAL
VAVANGKFFGGGMMIAPDAELTDGQFDIVILRAAGKLKLIWDIRLLYGGRHRNHPAITILRGKKVVVEPLDDVEKNGALV
DIDGESPGRIPATFEILPGALTLRY

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=298, Percent_Identity=25.1677852348993, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 36701; Mature: 36701

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSTAPLAPPAGAGFNAGKRSSVHARRRGGCVLKVGVVLNPIAGGGRLKRHWPEVAASL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEECEEEECCCCCCCHHHHHHHHHHHH
KKHFGDFELRETQAEGDAERLAIDLAATGFDLVIAAGGDGTASEVADGLLQAFEESGRTT
HHHCCCCEEEECCCCCCHHHEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
ELGLLPCGTGIDFARGLGLPKAVDATLKRIAGAEGRKVDAGRICYIDDHGALASRHFINI
EEEEEECCCCCHHHHCCCCCHHHHHHHHHHCCCCCCEECCCEEEEEECCCCCHHHHEEEE
ASLGLSGATDRAVNADKRKGKMSAKALFLWRTVVEFIRYRFQHVRITVDDGAPVEARMAL
EECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHEEEE
VAVANGKFFGGGMMIAPDAELTDGQFDIVILRAAGKLKLIWDIRLLYGGRHRNHPAITIL
EEEECCEEECCCEEECCCCCCCCCCEEEEEEECCCCEEEEEEEEEEECCCCCCCCEEEEE
RGKKVVVEPLDDVEKNGALVDIDGESPGRIPATFEILPGALTLRY
ECCEEEECCCHHHHHCCCEEEECCCCCCCCCEEEEECCCEEEECC
>Mature Secondary Structure
MLQSTAPLAPPAGAGFNAGKRSSVHARRRGGCVLKVGVVLNPIAGGGRLKRHWPEVAASL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEECEEEECCCCCCCHHHHHHHHHHHH
KKHFGDFELRETQAEGDAERLAIDLAATGFDLVIAAGGDGTASEVADGLLQAFEESGRTT
HHHCCCCEEEECCCCCCHHHEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
ELGLLPCGTGIDFARGLGLPKAVDATLKRIAGAEGRKVDAGRICYIDDHGALASRHFINI
EEEEEECCCCCHHHHCCCCCHHHHHHHHHHCCCCCCEECCCEEEEEECCCCCHHHHEEEE
ASLGLSGATDRAVNADKRKGKMSAKALFLWRTVVEFIRYRFQHVRITVDDGAPVEARMAL
EECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHEEEE
VAVANGKFFGGGMMIAPDAELTDGQFDIVILRAAGKLKLIWDIRLLYGGRHRNHPAITIL
EEEECCEEECCCEEECCCCCCCCCCEEEEEEECCCCEEEEEEEEEEECCCCCCCCEEEEE
RGKKVVVEPLDDVEKNGALVDIDGESPGRIPATFEILPGALTLRY
ECCEEEECCCHHHHHCCCEEEECCCCCCCCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]