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The map label for this gene is emrB [H]

Identifier: 13472329

GI number: 13472329

Start: 2094757

End: 2096313

Strand: Reverse

Name: emrB [H]

Synonym: mll2589

Alternate gene names: 13472329

Gene position: 2096313-2094757 (Counterclockwise)

Preceding gene: 13472330

Following gene: 13472326

Centisome position: 29.79

GC content: 64.1

Gene sequence:

>1557_bases
ATGTCGGAGCGCCCGCAATCCTTCTTCTCCAGCATCCTGCCGTTCATGGTGATGTGCGTGGGGATGTTCATCGCGCTGCT
CGACATCCAGATCGTGGCGTCGTCGCTGCAGGACATTGGCGGCGGGCTGTCGGCGGCGCAGGACCAGATCGGCTGGGTGC
AGACATCCTATCTGGTGGCGGAAATCATCGTCATCCCGCTGTCGGGCTGGCTGACAAGGGTGTTTTCGACACGCTGGCTG
TTCACCATTTCGGCGGCCGGCTTCACATTGGCCAGCATGCTGTGCGGTTTCGCCTGGAGCATCGAAAGCATGATCGTGTT
CCGCGCGCTGCAGGGGCTGCTCGGCGCGTCGATGATCCCGACGGTGTTCACCTCATCGTTCCACTATTTCCAGGGGCCGA
GGCGGGTCTATGCGGCCGCGGTGGTCGGCAGCATCGCCTCGATCGCGCCGACGCTCGGGCCGGTCATCGGCGGCTGGATC
ACCGACACGCTCAACTGGCACTGGCTGTTCTATGTCAACGTCATTCCGGGCACCATCATCACCATCCTCGTGGCAGTGCT
GGTCAAGATCGACAAGGCCGATCCGTCGCTGCTGAAGGGCGCGGACTATATCGGTATCGTGCTGATGGCGGTCAGCCTGG
GCACGGCGGAATACGTGCTGGAGGAAGGTTCGCGCTGGAACTGGTTCGACGACGCGACAATCCGCAATGGCGCCATCGTT
GCCGGCATCACCATGGTCCTGTTCGTCATCCGCAGCCTGACCTATTCGCAGCCGGTGGTCGATTTCCGCGCCTTCGGCAA
CCGCAATTTCGCCATTGGCTGCTTTCTGTCCTTCGTCACCGGCATCGGCATCTTCGCCACGATCTATCTGACGCCGCTGT
TCCTCGGCTATGTCAGGGGCTACGACGCCTTGCAGACCGGGCTTGCGGTGTTCTCGACCGGCGTCGCCTCGATGGTCGGC
GTGCCCGTCTACATCTTTCTCGCCAAGCGCTTCGACACGCGCTGGCTGATGATGTTCGGTCTGGCCTCCTTCGGCGCCTC
GATGTGGAGTTTCAGCGCCATCACCAGCCAGTGGGGCGCGGCCGAGCTGCTCATCCCGCAGCTGTTCCGCGGCTTCCCGC
AGGTCTTCGCCGTGGCGCCGAGCGTCAATCTCGGCCTCGGCAGCCTGCCGCCGGAGCGGCTGAAATACGCCAGCGGCCTG
TTCAACACGATGCGCAATCTCGGCGGCGCGGTCGGCATCGCCATTTGCGGCGCGATCCTGAACGACCGCACCAACTTCCA
TTTCCTGACGATCGCCTCGCACCTGACGCCGCAGAACGAGGCCGCGATGCGGGTCGTCGACACTATCGCGCTGCGCTACG
GCCAACTGCCCGGCGCCGTCGCCGCCGGCCATACCGCCTCGCTGAAACAGCTGTGGGCGCTCGCCTATCGCGAGGCTTCG
ACCATGGCCTATGCCGATGCTTTCCTGGTGATCATGGCCGCCTTCGTCATCGCGACCGCGCTGGTACCGTTCCTGCGCAA
CGTCACGCCAAAGGCACCGCCGCCGGACGCCCATTGA

Upstream 100 bases:

>100_bases
GTCCGCATCACCATCGACCCGGACCAGGCGCGGAAGGTCGCGCTGCGGCCAGGCCTCTCCACCGTCGTGACCGTCGATAC
CGGCGCGCACTGAGGGCATC

Downstream 100 bases:

>100_bases
GGTGGACCACCGCACCGGCTTGCGGATGGATGCAGGCCGATGGGCTAGTCGCCCGCGCGGGCGAGCAGCAGCGCGCGTTC
CCTGTCATTGCCGGTCAATT

Product: transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSERPQSFFSSILPFMVMCVGMFIALLDIQIVASSLQDIGGGLSAAQDQIGWVQTSYLVAEIIVIPLSGWLTRVFSTRWL
FTISAAGFTLASMLCGFAWSIESMIVFRALQGLLGASMIPTVFTSSFHYFQGPRRVYAAAVVGSIASIAPTLGPVIGGWI
TDTLNWHWLFYVNVIPGTIITILVAVLVKIDKADPSLLKGADYIGIVLMAVSLGTAEYVLEEGSRWNWFDDATIRNGAIV
AGITMVLFVIRSLTYSQPVVDFRAFGNRNFAIGCFLSFVTGIGIFATIYLTPLFLGYVRGYDALQTGLAVFSTGVASMVG
VPVYIFLAKRFDTRWLMMFGLASFGASMWSFSAITSQWGAAELLIPQLFRGFPQVFAVAPSVNLGLGSLPPERLKYASGL
FNTMRNLGGAVGIAICGAILNDRTNFHFLTIASHLTPQNEAAMRVVDTIALRYGQLPGAVAAGHTASLKQLWALAYREAS
TMAYADAFLVIMAAFVIATALVPFLRNVTPKAPPPDAH

Sequences:

>Translated_518_residues
MSERPQSFFSSILPFMVMCVGMFIALLDIQIVASSLQDIGGGLSAAQDQIGWVQTSYLVAEIIVIPLSGWLTRVFSTRWL
FTISAAGFTLASMLCGFAWSIESMIVFRALQGLLGASMIPTVFTSSFHYFQGPRRVYAAAVVGSIASIAPTLGPVIGGWI
TDTLNWHWLFYVNVIPGTIITILVAVLVKIDKADPSLLKGADYIGIVLMAVSLGTAEYVLEEGSRWNWFDDATIRNGAIV
AGITMVLFVIRSLTYSQPVVDFRAFGNRNFAIGCFLSFVTGIGIFATIYLTPLFLGYVRGYDALQTGLAVFSTGVASMVG
VPVYIFLAKRFDTRWLMMFGLASFGASMWSFSAITSQWGAAELLIPQLFRGFPQVFAVAPSVNLGLGSLPPERLKYASGL
FNTMRNLGGAVGIAICGAILNDRTNFHFLTIASHLTPQNEAAMRVVDTIALRYGQLPGAVAAGHTASLKQLWALAYREAS
TMAYADAFLVIMAAFVIATALVPFLRNVTPKAPPPDAH
>Mature_517_residues
SERPQSFFSSILPFMVMCVGMFIALLDIQIVASSLQDIGGGLSAAQDQIGWVQTSYLVAEIIVIPLSGWLTRVFSTRWLF
TISAAGFTLASMLCGFAWSIESMIVFRALQGLLGASMIPTVFTSSFHYFQGPRRVYAAAVVGSIASIAPTLGPVIGGWIT
DTLNWHWLFYVNVIPGTIITILVAVLVKIDKADPSLLKGADYIGIVLMAVSLGTAEYVLEEGSRWNWFDDATIRNGAIVA
GITMVLFVIRSLTYSQPVVDFRAFGNRNFAIGCFLSFVTGIGIFATIYLTPLFLGYVRGYDALQTGLAVFSTGVASMVGV
PVYIFLAKRFDTRWLMMFGLASFGASMWSFSAITSQWGAAELLIPQLFRGFPQVFAVAPSVNLGLGSLPPERLKYASGLF
NTMRNLGGAVGIAICGAILNDRTNFHFLTIASHLTPQNEAAMRVVDTIALRYGQLPGAVAAGHTASLKQLWALAYREAST
MAYADAFLVIMAAFVIATALVPFLRNVTPKAPPPDAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=470, Percent_Identity=28.5106382978723, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=27.1084337349398, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790195, Length=428, Percent_Identity=23.8317757009346, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788392, Length=408, Percent_Identity=25, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=23.8442822384428, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55985; Mature: 55854

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SERPQSFFSSILPFMVMCVGMFIALLDIQIVASSLQDIGGGLSAAQDQIGWVQTSYLVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]