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The map label for this gene is pbuE [H]

Identifier: 13472271

GI number: 13472271

Start: 2036456

End: 2037634

Strand: Reverse

Name: pbuE [H]

Synonym: mll2510

Alternate gene names: 13472271

Gene position: 2037634-2036456 (Counterclockwise)

Preceding gene: 13472273

Following gene: 13472269

Centisome position: 28.96

GC content: 70.14

Gene sequence:

>1179_bases
ATGGACAAGCGCCTCTTCTGGCTTGCGCTCGGATCGTTCACGATCTCGACCGAGGGCTTCGTCATCTCCAGCCTTCTGCC
CGACATTGCCAGGGATGCCGGCATTTCCATTCCGCTCGCCGGCACGCTGATCACCGCCTTCGCGCTCGCCTACGCCATCG
GCACGCCGATCCTGGCGACGCTGACAGGCGAATGGGACCGGCGCCGCGTCATCCTGTGGACGCTGGTGTTCTTCGTCATC
GGCAACATTGCCGCCGCCTTGAGTTCCTCCTTCGAAGTGCTGTTGATCGCGCGCGTCATCATGGCGCTGTCGTCGGGCCT
GTTCGCCGCGACCGCGCAGGGTACGGCGGTGGCGCTGGTCGATGATCATCACCGGGCACGCGCCATCGCCGTTGTCGTCG
GCGGCACCACAGTGGCCGTCGCCGTCGGTGCGCCGCTCGGCGCGCTGGTCGCGACGATCGCCGGCTGGCGCGGCACCTTC
TATGCCATTGCCGGGCTCGGCGCGCTTGCCGGCGCCATCCTGTGGTACCGGCTGCCGCGCGGCATCGTCGGCACCAGGCT
GCCGCTGAAGCGCCGGCTGGCGGCCGCCATGCGGCCCGGGGTGATGCCGATCCTGGTGACGACATTGCTGGCGCTGACCG
GCGCCTTCACCGTCTTTGCCTATGTCGCGCCGCTCGCCATCGAGGGCGCCGGGCTCAGCCAGATCGCGCTGCCCGGCATG
CTGCTCGCCTTCGGCGTCGGCGCCGTCATCGGCAACATTGCCGGCGGCCAGGCGGCCGATCGTTTCGGTGCCACCCGCAC
CGTCGGCTGGTCGCTGACGCTGAGTGCGGCCATGCTGGTGATGCTGTCGGTCGTGCCGGCCTTCCTGCCGCAGCACATTG
CCGGGCCGGCGCTGATGGCAATGATGGTGCCGTGGGGCATCGTCGGCTGGGCGTTCCCGCCGGCGCAGGCCAGCCGCATC
ATCAAGCTGGCGCCTGATGCCGCGCCGATCGTGCTGTCGCTCAACGGGTCGGCGCTCTATCTCGGGGTGGCGCTTGGCGC
GGTGGTCGGCGGTGGTGTGCTGCGCTACGGCGCGCCGGTCGATCTCGGTTTGGTCGCGGCTGCCTTCCCGTTGATCGGGC
TGGGCATCGTGCTGGCCGGCCGCTGGGCGGCACGGCCGGTCGCGATGCCGGCCGAATAG

Upstream 100 bases:

>100_bases
CAGGGCTGTCAGGATGGTTCAGCTATTTTGCCCTGGCTTCATTGACATCATGCCCTGTTATGTCCAATTGTTCAATAACT
TTTGAACTAAGGACCCTGCC

Downstream 100 bases:

>100_bases
TCTCTTCGAAAGCCGTCGGCTGCCAATCCATTTTGACGCAATTCCCGACGGAAAACCGTTTCACACTTTTCCTGGCATTG
CTTAGGTAGCCGGCGGCAAA

Product: transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MDKRLFWLALGSFTISTEGFVISSLLPDIARDAGISIPLAGTLITAFALAYAIGTPILATLTGEWDRRRVILWTLVFFVI
GNIAAALSSSFEVLLIARVIMALSSGLFAATAQGTAVALVDDHHRARAIAVVVGGTTVAVAVGAPLGALVATIAGWRGTF
YAIAGLGALAGAILWYRLPRGIVGTRLPLKRRLAAAMRPGVMPILVTTLLALTGAFTVFAYVAPLAIEGAGLSQIALPGM
LLAFGVGAVIGNIAGGQAADRFGATRTVGWSLTLSAAMLVMLSVVPAFLPQHIAGPALMAMMVPWGIVGWAFPPAQASRI
IKLAPDAAPIVLSLNGSALYLGVALGAVVGGGVLRYGAPVDLGLVAAAFPLIGLGIVLAGRWAARPVAMPAE

Sequences:

>Translated_392_residues
MDKRLFWLALGSFTISTEGFVISSLLPDIARDAGISIPLAGTLITAFALAYAIGTPILATLTGEWDRRRVILWTLVFFVI
GNIAAALSSSFEVLLIARVIMALSSGLFAATAQGTAVALVDDHHRARAIAVVVGGTTVAVAVGAPLGALVATIAGWRGTF
YAIAGLGALAGAILWYRLPRGIVGTRLPLKRRLAAAMRPGVMPILVTTLLALTGAFTVFAYVAPLAIEGAGLSQIALPGM
LLAFGVGAVIGNIAGGQAADRFGATRTVGWSLTLSAAMLVMLSVVPAFLPQHIAGPALMAMMVPWGIVGWAFPPAQASRI
IKLAPDAAPIVLSLNGSALYLGVALGAVVGGGVLRYGAPVDLGLVAAAFPLIGLGIVLAGRWAARPVAMPAE
>Mature_392_residues
MDKRLFWLALGSFTISTEGFVISSLLPDIARDAGISIPLAGTLITAFALAYAIGTPILATLTGEWDRRRVILWTLVFFVI
GNIAAALSSSFEVLLIARVIMALSSGLFAATAQGTAVALVDDHHRARAIAVVVGGTTVAVAVGAPLGALVATIAGWRGTF
YAIAGLGALAGAILWYRLPRGIVGTRLPLKRRLAAAMRPGVMPILVTTLLALTGAFTVFAYVAPLAIEGAGLSQIALPGM
LLAFGVGAVIGNIAGGQAADRFGATRTVGWSLTLSAAMLVMLSVVPAFLPQHIAGPALMAMMVPWGIVGWAFPPAQASRI
IKLAPDAAPIVLSLNGSALYLGVALGAVVGGGVLRYGAPVDLGLVAAAFPLIGLGIVLAGRWAARPVAMPAE

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=335, Percent_Identity=33.4328358208955, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786595, Length=266, Percent_Identity=29.6992481203008, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787808, Length=397, Percent_Identity=28.2115869017632, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI145693198, Length=238, Percent_Identity=29.8319327731092, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40039; Mature: 40039

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRLFWLALGSFTISTEGFVISSLLPDIARDAGISIPLAGTLITAFALAYAIGTPILAT
CCCEEEEEEECCEEECCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LTGEWDRRRVILWTLVFFVIGNIAAALSSSFEVLLIARVIMALSSGLFAATAQGTAVALV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEE
DDHHRARAIAVVVGGTTVAVAVGAPLGALVATIAGWRGTFYAIAGLGALAGAILWYRLPR
CCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
GIVGTRLPLKRRLAAAMRPGVMPILVTTLLALTGAFTVFAYVAPLAIEGAGLSQIALPGM
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLAFGVGAVIGNIAGGQAADRFGATRTVGWSLTLSAAMLVMLSVVPAFLPQHIAGPALMA
HHHHHHHHHHHCCCCCCHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MMVPWGIVGWAFPPAQASRIIKLAPDAAPIVLSLNGSALYLGVALGAVVGGGVLRYGAPV
HHHHHHHHHCCCCCHHHCCEEEECCCCCCEEEEECCCEEHHHHHHHHHHCCCHHCCCCCC
DLGLVAAAFPLIGLGIVLAGRWAARPVAMPAE
HHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
>Mature Secondary Structure
MDKRLFWLALGSFTISTEGFVISSLLPDIARDAGISIPLAGTLITAFALAYAIGTPILAT
CCCEEEEEEECCEEECCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LTGEWDRRRVILWTLVFFVIGNIAAALSSSFEVLLIARVIMALSSGLFAATAQGTAVALV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEE
DDHHRARAIAVVVGGTTVAVAVGAPLGALVATIAGWRGTFYAIAGLGALAGAILWYRLPR
CCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
GIVGTRLPLKRRLAAAMRPGVMPILVTTLLALTGAFTVFAYVAPLAIEGAGLSQIALPGM
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLAFGVGAVIGNIAGGQAADRFGATRTVGWSLTLSAAMLVMLSVVPAFLPQHIAGPALMA
HHHHHHHHHHHCCCCCCHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MMVPWGIVGWAFPPAQASRIIKLAPDAAPIVLSLNGSALYLGVALGAVVGGGVLRYGAPV
HHHHHHHHHCCCCCHHHCCEEEECCCCCCEEEEECCCEEHHHHHHHHHHCCCHHCCCCCC
DLGLVAAAFPLIGLGIVLAGRWAARPVAMPAE
HHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]