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The map label for this gene is yogA [H]

Identifier: 13471815

GI number: 13471815

Start: 1572496

End: 1573146

Strand: Direct

Name: yogA [H]

Synonym: mlr1913

Alternate gene names: 13471815

Gene position: 1572496-1573146 (Clockwise)

Preceding gene: 13471814

Following gene: 13471825

Centisome position: 22.35

GC content: 67.28

Gene sequence:

>651_bases
ATGCCCGCGCAGGCCTTCACCAAAGCTCCCGCGGGCTATACGCACGCGGAAGCCGCGGCGCTGACCTGTACCGGCGTCAC
CGCCTGGCGAGGCCTGGTCGTATCCGGCAAGGTCAAGCCCGGCGACACGGTGCTGGTTCTCGGAACCGGCAGCGTGTCGC
TGTTTGCGCTGCAATTCGCCAAGGCCGCCGGCGCCCGGGTCATCGCCACCTCGTCCGATGAGGAAAAGCTGGAGCGGCTA
AGCCGCCTCGGCGCCGACGCCGTCATCAACTACAAGGCCGTGCCGGACTGGAGCCGCAAGGTTCGGGAATTGACGGGCGG
ACGCGGCGTCGACCACGTGATCGAGGTCGGCGGCCCGGCCACGCTGGCGCAGTCCATAGCCGCCTGCCGCACAGGCGGCC
ACATCGCCCTGATCGGCGTGTTGACCGGCTTTGCGGCCGATGTCTCGATCCCGGCCGTATTCTCGAACCAGATCCGCATC
AGCGGCATTTCGATCGGCAGCCGGGCGGACCAGGAGGACATGATCCGTGCGATAGACGTCAATCGCATCAAGCCCGTCAT
CGACCGCCGCTTCCCGCTGCGCGAGATCGCCGCCGCCTTCACGCACTACGCAGCGCGGAAGCATTTCGGCAAGGTCTGCC
TGGAGCTTTGA

Upstream 100 bases:

>100_bases
AGTGTCTTCTATCCTTGGTGGCTGGACGGCGACATGACGCCCGCCACCAGGCGCGACATTCCAGGAGAGTTTCGACGGCT
TCGCCAGCGAATATGTCTGC

Downstream 100 bases:

>100_bases
GCCCTGCCTGACCGCAGCCGTCGCGAAACGGCTGCAACGTTCCCTCACGCATCGGCCGCCTGGACCGTCAGCGCCCGCAT
GACTTCGGCTTCGAAGGCGC

Product: alcohol dehydrogenase

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MPAQAFTKAPAGYTHAEAAALTCTGVTAWRGLVVSGKVKPGDTVLVLGTGSVSLFALQFAKAAGARVIATSSDEEKLERL
SRLGADAVINYKAVPDWSRKVRELTGGRGVDHVIEVGGPATLAQSIAACRTGGHIALIGVLTGFAADVSIPAVFSNQIRI
SGISIGSRADQEDMIRAIDVNRIKPVIDRRFPLREIAAAFTHYAARKHFGKVCLEL

Sequences:

>Translated_216_residues
MPAQAFTKAPAGYTHAEAAALTCTGVTAWRGLVVSGKVKPGDTVLVLGTGSVSLFALQFAKAAGARVIATSSDEEKLERL
SRLGADAVINYKAVPDWSRKVRELTGGRGVDHVIEVGGPATLAQSIAACRTGGHIALIGVLTGFAADVSIPAVFSNQIRI
SGISIGSRADQEDMIRAIDVNRIKPVIDRRFPLREIAAAFTHYAARKHFGKVCLEL
>Mature_215_residues
PAQAFTKAPAGYTHAEAAALTCTGVTAWRGLVVSGKVKPGDTVLVLGTGSVSLFALQFAKAAGARVIATSSDEEKLERLS
RLGADAVINYKAVPDWSRKVRELTGGRGVDHVIEVGGPATLAQSIAACRTGGHIALIGVLTGFAADVSIPAVFSNQIRIS
GISIGSRADQEDMIRAIDVNRIKPVIDRRFPLREIAAAFTHYAARKHFGKVCLEL

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=234, Percent_Identity=29.4871794871795, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI22538444, Length=234, Percent_Identity=29.4871794871795, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI194239674, Length=199, Percent_Identity=27.6381909547739, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI13236495, Length=199, Percent_Identity=27.6381909547739, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI197927207, Length=175, Percent_Identity=27.4285714285714, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI47519420, Length=217, Percent_Identity=27.1889400921659, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI87081918, Length=210, Percent_Identity=29.5238095238095, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319520, Length=188, Percent_Identity=30.3191489361702, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=182, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24581345, Length=226, Percent_Identity=26.5486725663717, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 22763; Mature: 22631

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQAFTKAPAGYTHAEAAALTCTGVTAWRGLVVSGKVKPGDTVLVLGTGSVSLFALQFA
CCCCCCCCCCCCCCCCHHHEEEECCHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHH
KAAGARVIATSSDEEKLERLSRLGADAVINYKAVPDWSRKVRELTGGRGVDHVIEVGGPA
HHCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHEECCCHH
TLAQSIAACRTGGHIALIGVLTGFAADVSIPAVFSNQIRISGISIGSRADQEDMIRAIDV
HHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHH
NRIKPVIDRRFPLREIAAAFTHYAARKHFGKVCLEL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAQAFTKAPAGYTHAEAAALTCTGVTAWRGLVVSGKVKPGDTVLVLGTGSVSLFALQFA
CCCCCCCCCCCCCCCHHHEEEECCHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHH
KAAGARVIATSSDEEKLERLSRLGADAVINYKAVPDWSRKVRELTGGRGVDHVIEVGGPA
HHCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHEECCCHH
TLAQSIAACRTGGHIALIGVLTGFAADVSIPAVFSNQIRISGISIGSRADQEDMIRAIDV
HHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHH
NRIKPVIDRRFPLREIAAAFTHYAARKHFGKVCLEL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]