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The map label for this gene is rutR [H]

Identifier: 13471610

GI number: 13471610

Start: 1357773

End: 1358408

Strand: Direct

Name: rutR [H]

Synonym: mlr1634

Alternate gene names: 13471610

Gene position: 1357773-1358408 (Clockwise)

Preceding gene: 13471604

Following gene: 13471612

Centisome position: 19.3

GC content: 63.52

Gene sequence:

>636_bases
GTGAACACCGGCACAGAAGCCCCTCGCCGCACGCGTATCCAGCAGGAAAAGCGCGAACTCATCCTGGAGGCGGCGCTCGA
AGTGTTCTCCACCCATGGCTTCCGCGGCTCGACCATCGACCAGATCGCCGAAGCGGCCGGCATGTCGAAGCCCAACCTGC
TCTATTACTTCCGCCGCAAGGAAGACATTCACGAGACATTGATGCAGCGCCTGCTCGACACCTGGCTGGCGCCGCTGCGC
GAACTCGACGACATCGGCGATCCGATGACTGAGCTGCGCAGCTACATCAGGCGCAAGCTTGAAATGGCGCGCGATTTCCC
GCGCGAGAGCCGGCTGTTCGCCAACGAAATCCTGCAGGGCGCGCCGCGCATCATGCCGCTGCTGGCGGGCGAGTTGAAAA
CGCTGGTCGATGAGAAAGCCGCCGTCATCAAGGGTTGGATGCGCGCCGGCAAGATCGCCAAGACCGATCCCTGGCACCTG
ATCTTCTCGATCTGGGCAACGACGCAGCACTACGCCGATTTCGACGTCCAGGTGCGCGCCGTGCTCGGGCCGAACCGCGG
CGGCGACGGCCGCTTCGAGGACGCGGCAAGATTCTTGGAGCAATTGTTTCTTGAGGGGCTGAAGCCGAGGGGCTGA

Upstream 100 bases:

>100_bases
TACTAGCGGAAATGCGGCTCCTGAAGTGCGTTCCACAGCCTAGGGAAAAACTGGTCCAGAGAATTGGTCCAGATGACCGC
CAACATGAGGGTTCGCAACG

Downstream 100 bases:

>100_bases
CCCCCCGCCCACGACGGCTCAGGCGCTACGCCGCTCGGCCGGCATCGCGTCCAGCAAGGATTGCAGCTTGGCGATCGAGT
TCTGTTCGGCGAGCGGCGTG

Product: transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNTGTEAPRRTRIQQEKRELILEAALEVFSTHGFRGSTIDQIAEAAGMSKPNLLYYFRRKEDIHETLMQRLLDTWLAPLR
ELDDIGDPMTELRSYIRRKLEMARDFPRESRLFANEILQGAPRIMPLLAGELKTLVDEKAAVIKGWMRAGKIAKTDPWHL
IFSIWATTQHYADFDVQVRAVLGPNRGGDGRFEDAARFLEQLFLEGLKPRG

Sequences:

>Translated_211_residues
MNTGTEAPRRTRIQQEKRELILEAALEVFSTHGFRGSTIDQIAEAAGMSKPNLLYYFRRKEDIHETLMQRLLDTWLAPLR
ELDDIGDPMTELRSYIRRKLEMARDFPRESRLFANEILQGAPRIMPLLAGELKTLVDEKAAVIKGWMRAGKIAKTDPWHL
IFSIWATTQHYADFDVQVRAVLGPNRGGDGRFEDAARFLEQLFLEGLKPRG
>Mature_211_residues
MNTGTEAPRRTRIQQEKRELILEAALEVFSTHGFRGSTIDQIAEAAGMSKPNLLYYFRRKEDIHETLMQRLLDTWLAPLR
ELDDIGDPMTELRSYIRRKLEMARDFPRESRLFANEILQGAPRIMPLLAGELKTLVDEKAAVIKGWMRAGKIAKTDPWHL
IFSIWATTQHYADFDVQVRAVLGPNRGGDGRFEDAARFLEQLFLEGLKPRG

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=202, Percent_Identity=48.019801980198, Blast_Score=207, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24253; Mature: 24253

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTGTEAPRRTRIQQEKRELILEAALEVFSTHGFRGSTIDQIAEAAGMSKPNLLYYFRRK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEHHHHH
EDIHETLMQRLLDTWLAPLRELDDIGDPMTELRSYIRRKLEMARDFPRESRLFANEILQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
APRIMPLLAGELKTLVDEKAAVIKGWMRAGKIAKTDPWHLIFSIWATTQHYADFDVQVRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
VLGPNRGGDGRFEDAARFLEQLFLEGLKPRG
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNTGTEAPRRTRIQQEKRELILEAALEVFSTHGFRGSTIDQIAEAAGMSKPNLLYYFRRK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEHHHHH
EDIHETLMQRLLDTWLAPLRELDDIGDPMTELRSYIRRKLEMARDFPRESRLFANEILQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
APRIMPLLAGELKTLVDEKAAVIKGWMRAGKIAKTDPWHLIFSIWATTQHYADFDVQVRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
VLGPNRGGDGRFEDAARFLEQLFLEGLKPRG
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]