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The map label for this gene is ddl

Identifier: 13471546

GI number: 13471546

Start: 1289898

End: 1290824

Strand: Reverse

Name: ddl

Synonym: mll1551

Alternate gene names: 13471546

Gene position: 1290824-1289898 (Counterclockwise)

Preceding gene: 13471547

Following gene: 13471545

Centisome position: 18.35

GC content: 58.9

Gene sequence:

>927_bases
ATGAAAAGCAAGCATGTGGCCGTCCTGCTGGGAGGTTTTTCGTCCGAGCGGCCCGTGTCCCTGTCTTCCGGAAAGGCCTG
TGCCGATGCGCTGGAGAATGAGGGTTACCAGGTCACCCGCGTCGATGTCGGGCGCGATGTCGGCTCGGTGCTTGCCGAAC
TCAAGCCTGACGTCGCCTTCAATGCGTTGCACGGCCCGTTCGGCGAGGACGGCACCATCCAGGGCATCCTCGAATATCTC
GGCATTCCCTACACCCATTCCGGCGTGCTCGCCTCGGCGCTCGCCATGAACAAGGAGCAGGCGAAGAAGATCGTCAAAAC
GGTGGGTGTGCCTGTCGCCGAATCGAAGGTCGCCAATCGCTTCGCCATCCAGAACAAGCATCCGATGAAGCCGCCCTATG
TGATCAAGCCGGTCAATGAGGGGTCGAGCTTCGGCGTCGTCATCGTGTCGGAGGGGCAGTCGCATCCGCCGCAGGTCGTA
GGCTCGTCCGAGTGGAAATATGGCGATACCGTCATGGTCGAGCGCTATATCCACGGGCGCGAGCTGACCTGCGCCGTCAT
GGGTGATGTGGCGCTCGGCGTCTGCGAGATCATTCCGACCGGCCATTCCTTCTACGATTACGATTCAAAATATGTCGCGG
GCGGATCAAAACACGAATGCCCCGCAAAAGTTTCACCGAATATTTACCAAAAAATACAGACACTGGCGCTCAAGGCTCAC
CAAGCTGTCGGCTGTCGGGGCGTTTCCCGGTCGGACTTCCGTTATGACGATCGTCACTCCGAAAATGGCGAAGTTGTATG
GCTCGAGGTCAACACTCAGCCGGGCATGACGCCGACGTCTCTGGTGCCTGAAATTGCGGCCCATGCCGGGCATTCGTTTG
GTGAGTTGTTGAGTTGGATGGTGGAGGACGCTTCGTGTCTGCGTTGA

Upstream 100 bases:

>100_bases
TACCCGCGTTTTCCGTCTGGGGGGCAACCTGCCGGGAGACTCGGACTCAATGTTGCGGAAATCTTGGTTTTTGGTCCGCC
GTGAGAGGGGATGACGGGGA

Downstream 100 bases:

>100_bases
AATGGGGACAAGGCAAGGGGAAGGGCGCGGCCGGGCCGTCGCTGTTCGGCTTGTCGTTGTCATCAGGCCATTTCGTGCTG
CCGCGCATGCTTCGCCGCCC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKSKHVAVLLGGFSSERPVSLSSGKACADALENEGYQVTRVDVGRDVGSVLAELKPDVAFNALHGPFGEDGTIQGILEYL
GIPYTHSGVLASALAMNKEQAKKIVKTVGVPVAESKVANRFAIQNKHPMKPPYVIKPVNEGSSFGVVIVSEGQSHPPQVV
GSSEWKYGDTVMVERYIHGRELTCAVMGDVALGVCEIIPTGHSFYDYDSKYVAGGSKHECPAKVSPNIYQKIQTLALKAH
QAVGCRGVSRSDFRYDDRHSENGEVVWLEVNTQPGMTPTSLVPEIAAHAGHSFGELLSWMVEDASCLR

Sequences:

>Translated_308_residues
MKSKHVAVLLGGFSSERPVSLSSGKACADALENEGYQVTRVDVGRDVGSVLAELKPDVAFNALHGPFGEDGTIQGILEYL
GIPYTHSGVLASALAMNKEQAKKIVKTVGVPVAESKVANRFAIQNKHPMKPPYVIKPVNEGSSFGVVIVSEGQSHPPQVV
GSSEWKYGDTVMVERYIHGRELTCAVMGDVALGVCEIIPTGHSFYDYDSKYVAGGSKHECPAKVSPNIYQKIQTLALKAH
QAVGCRGVSRSDFRYDDRHSENGEVVWLEVNTQPGMTPTSLVPEIAAHAGHSFGELLSWMVEDASCLR
>Mature_308_residues
MKSKHVAVLLGGFSSERPVSLSSGKACADALENEGYQVTRVDVGRDVGSVLAELKPDVAFNALHGPFGEDGTIQGILEYL
GIPYTHSGVLASALAMNKEQAKKIVKTVGVPVAESKVANRFAIQNKHPMKPPYVIKPVNEGSSFGVVIVSEGQSHPPQVV
GSSEWKYGDTVMVERYIHGRELTCAVMGDVALGVCEIIPTGHSFYDYDSKYVAGGSKHECPAKVSPNIYQKIQTLALKAH
QAVGCRGVSRSDFRYDDRHSENGEVVWLEVNTQPGMTPTSLVPEIAAHAGHSFGELLSWMVEDASCLR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=312, Percent_Identity=37.5, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1786579, Length=354, Percent_Identity=30.225988700565, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLB_RHILO (Q98KB6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103112.1
- ProteinModelPortal:   Q98KB6
- SMR:   Q98KB6
- GeneID:   1225775
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1551
- NMPDR:   fig|266835.1.peg.1217
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- ProtClustDB:   PRK01372
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33179; Mature: 33179

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKHVAVLLGGFSSERPVSLSSGKACADALENEGYQVTRVDVGRDVGSVLAELKPDVAF
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEE
NALHGPFGEDGTIQGILEYLGIPYTHSGVLASALAMNKEQAKKIVKTVGVPVAESKVANR
ECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
FAIQNKHPMKPPYVIKPVNEGSSFGVVIVSEGQSHPPQVVGSSEWKYGDTVMVERYIHGR
HEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCC
ELTCAVMGDVALGVCEIIPTGHSFYDYDSKYVAGGSKHECPAKVSPNIYQKIQTLALKAH
EEEEEEHHHHHHHHHHEECCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
QAVGCRGVSRSDFRYDDRHSENGEVVWLEVNTQPGMTPTSLVPEIAAHAGHSFGELLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VEDASCLR
HHHHHHCC
>Mature Secondary Structure
MKSKHVAVLLGGFSSERPVSLSSGKACADALENEGYQVTRVDVGRDVGSVLAELKPDVAF
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEE
NALHGPFGEDGTIQGILEYLGIPYTHSGVLASALAMNKEQAKKIVKTVGVPVAESKVANR
ECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
FAIQNKHPMKPPYVIKPVNEGSSFGVVIVSEGQSHPPQVVGSSEWKYGDTVMVERYIHGR
HEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCC
ELTCAVMGDVALGVCEIIPTGHSFYDYDSKYVAGGSKHECPAKVSPNIYQKIQTLALKAH
EEEEEEHHHHHHHHHHEECCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
QAVGCRGVSRSDFRYDDRHSENGEVVWLEVNTQPGMTPTSLVPEIAAHAGHSFGELLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VEDASCLR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968