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The map label for this gene is ligA

Identifier: 13471538

GI number: 13471538

Start: 1279308

End: 1281512

Strand: Reverse

Name: ligA

Synonym: mll1539

Alternate gene names: 13471538

Gene position: 1281512-1279308 (Counterclockwise)

Preceding gene: 13471539

Following gene: 13471537

Centisome position: 18.21

GC content: 64.35

Gene sequence:

>2205_bases
ATGGCCGAAAAACCTGTCGATTCGCTCAGCGAAAGCGAGGCTGAAGCCGAGCTGAAGCGCCTGGCGGAGGAGATCGCCGG
GCACGACCGGCGCTACCATGCCGAGGACGCGCCCACGATCACGGACGCCGAATATGATGCGCTGCGGCGGCGCAACCTTG
CCATCGAGGAGCGTTTTCCCGGCCTGGTGCGCGAGGATTCGCCGTCGCGCAGGGTCGGTGCGCCGCCGGCGGAGGGCTTT
GCCAAGGTGCGCCATGCCGTGCCGATGCTCAGCCTCGCCAAGGCCTATACCGACGAGGATGTCACCGATTTCATCGAGCG
CGGCCGGCGCTTCTTCGACCGCGACAAGGACCTCGATATCGCCTTCACCGCCGAACCGAAGATCGACGGGCTGTCGGCGT
CGTTGCGCTATGAGAATGGCGTGTTCGTGCAGGGCGCGACGCGCGGCGACGGCGCCGTCGGTGAGGACATCACCGCCAAT
CTCCGAACCATCGCCGATATCCCCGCGAAGCTGAAAGGCTCGGGCTGGCCGGATGTCATCGAGATACGCGGCGAGGTCTA
CATGACCTATGCGGAGTTCGAGGCGCTGAAAGCGCGCTCGGCGGCGGCCGGCGGCCAGGATTACGTCAATCCGCGCAACA
CGGCGGCCGGCTCGCTGCGCCAGAAGGATGCTTCGGTCACAGCCAGCCGCAATCTCAAATTCTTCGCCTATGCCTGGGGC
TTCACGACCGCGGATCCAGCGCCGACCCAGTATGAATCGGTGCAAAAATTCGCGGACTGGGGGTTCAAGATCAGCCCTTT
GATGGTGCGGGCGAAGTCGGTCGAGGAACTGGTCGCGCAGTACCATCTGATCGAGGAGCAACGCTCCTCGCTCGGCTATG
ACATTGATGGTGTCGTCTACAAGGTCGACCAGCTGGAGTTGCAGCGCCGCTGGGGTTTCGTCACCGGTGAACCGCGCTGG
GCGGTTGCCCACAAATTCCCGGCCGAACAGGCAATGACGACCGTGCAGAAGATCGACATCCAGGTTGGCCGTACCGGCAC
GCTGGCGCCGGTCGCAAGGCTTGCGCCCGTCACCGTTGGCGGCGTCGTGGTCGAAAATGTCACGCTGCACAATGAGGACT
ACATCAAGGGGTTCGACAGCAACGGCCTGCCGATCCGCGACGGCATCGACGTGCGCATCGGCGACACGGTGGTGATCCAG
CGGGCGGGAGACGTCATTCCGCAGATCGTCAGTGTCGTCATCGACAAGCGTCCGGCCGATGCCGTGCCTTACGAATTCCC
GCACACCTGTCCGGTCTGCGGCTCGCCGGCGACGCGCGAGATCAACGAGAAGACCGGCAAGGAGGATTCCCGCCGACGCT
GCACCGGCGAACTGATCTGCGCCGCGCAAGCCGTGGAAAGATTGCGCCATTTCGTCTCGCGCGGGGCGCTCGATATAGAG
GGACTGGGCGCCGAAAACATCGACACTTTCTTCAATGCCGGGCTGATCAAGACCGCCGCCGATATTTTTACGCTCAGGGA
TCGCCGTCCCGCCGTCACCAAGGCGCTGGCCGAGCGACGCGAGGAGCAGGCCAGGCAGCGCGAAGCGGCGTCCGGCAAGA
CGCGCAAGAACGTGCGCAGTGTCGAGGATCGCAACTATGAAGGCCTCGACAAGCTGTTCGCAGCCATCGATTCGCGCCGC
GAACCGGAGCTCGATCGCTTCATCTTCGCGCTCGGCATCCGCCACATCGGCGAGACGACGGCGGCCGTGCTCGCCAAGAC
CTTTTCCACCATCGAGGAACTGATCCGCGTCGGCAAAGAGACGGCGGCGGCGGAGGATCCGCACACCGTCTTCCCATCGG
TCAACGGCATCGGTGACACGGTGATCGATGCGCTGTGCGATTTCTTCGGCAATGAGCGCAATGACGACGTGCTCGACAAG
CTGCTCGAGCAGGTCAAGCCCAAGCCCTATATCGTCACCGTCTCGGCCGACAGCGAGGTCGCCGGCAAGACCATCGTCTT
CACCGGCACGCTGGAGAAGATGACGCGCTCGGAAGCCAAGGCGATGGCCGAACGTCTCGGCGCAAAGGTCGCCGGCTCGG
TTTCGGCCAAGACCGACCTGCTGGTCGCCGGACCCGGCGCCGGCTCCAAGCTCAAGCTTGCCAGCGAACTCGGCGTCGAA
GTCATCGACGAGGACACCTGGCTGCAACGGGTCGGCAAGGCGTGA

Upstream 100 bases:

>100_bases
GCTCCATCCTGCTGTTCAGGAATGATCTTTTCCGAAAAGCGGTTCCCACTTTTCGGGATCATGCTCTATTGTGGCGCGCC
ACCGTTCGGGGAAGAAAAGC

Downstream 100 bases:

>100_bases
TGGCGTTCAAAGGCTTCGGCCCAAAGGCCATTCCGTTCCTGAAGGCGCTGGATTTCCACCAGAGCCGGGAGTGGTTTTTG
GAGAATCGCGACCTGTTCGA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MAEKPVDSLSESEAEAELKRLAEEIAGHDRRYHAEDAPTITDAEYDALRRRNLAIEERFPGLVREDSPSRRVGAPPAEGF
AKVRHAVPMLSLAKAYTDEDVTDFIERGRRFFDRDKDLDIAFTAEPKIDGLSASLRYENGVFVQGATRGDGAVGEDITAN
LRTIADIPAKLKGSGWPDVIEIRGEVYMTYAEFEALKARSAAAGGQDYVNPRNTAAGSLRQKDASVTASRNLKFFAYAWG
FTTADPAPTQYESVQKFADWGFKISPLMVRAKSVEELVAQYHLIEEQRSSLGYDIDGVVYKVDQLELQRRWGFVTGEPRW
AVAHKFPAEQAMTTVQKIDIQVGRTGTLAPVARLAPVTVGGVVVENVTLHNEDYIKGFDSNGLPIRDGIDVRIGDTVVIQ
RAGDVIPQIVSVVIDKRPADAVPYEFPHTCPVCGSPATREINEKTGKEDSRRRCTGELICAAQAVERLRHFVSRGALDIE
GLGAENIDTFFNAGLIKTAADIFTLRDRRPAVTKALAERREEQARQREAASGKTRKNVRSVEDRNYEGLDKLFAAIDSRR
EPELDRFIFALGIRHIGETTAAVLAKTFSTIEELIRVGKETAAAEDPHTVFPSVNGIGDTVIDALCDFFGNERNDDVLDK
LLEQVKPKPYIVTVSADSEVAGKTIVFTGTLEKMTRSEAKAMAERLGAKVAGSVSAKTDLLVAGPGAGSKLKLASELGVE
VIDEDTWLQRVGKA

Sequences:

>Translated_734_residues
MAEKPVDSLSESEAEAELKRLAEEIAGHDRRYHAEDAPTITDAEYDALRRRNLAIEERFPGLVREDSPSRRVGAPPAEGF
AKVRHAVPMLSLAKAYTDEDVTDFIERGRRFFDRDKDLDIAFTAEPKIDGLSASLRYENGVFVQGATRGDGAVGEDITAN
LRTIADIPAKLKGSGWPDVIEIRGEVYMTYAEFEALKARSAAAGGQDYVNPRNTAAGSLRQKDASVTASRNLKFFAYAWG
FTTADPAPTQYESVQKFADWGFKISPLMVRAKSVEELVAQYHLIEEQRSSLGYDIDGVVYKVDQLELQRRWGFVTGEPRW
AVAHKFPAEQAMTTVQKIDIQVGRTGTLAPVARLAPVTVGGVVVENVTLHNEDYIKGFDSNGLPIRDGIDVRIGDTVVIQ
RAGDVIPQIVSVVIDKRPADAVPYEFPHTCPVCGSPATREINEKTGKEDSRRRCTGELICAAQAVERLRHFVSRGALDIE
GLGAENIDTFFNAGLIKTAADIFTLRDRRPAVTKALAERREEQARQREAASGKTRKNVRSVEDRNYEGLDKLFAAIDSRR
EPELDRFIFALGIRHIGETTAAVLAKTFSTIEELIRVGKETAAAEDPHTVFPSVNGIGDTVIDALCDFFGNERNDDVLDK
LLEQVKPKPYIVTVSADSEVAGKTIVFTGTLEKMTRSEAKAMAERLGAKVAGSVSAKTDLLVAGPGAGSKLKLASELGVE
VIDEDTWLQRVGKA
>Mature_733_residues
AEKPVDSLSESEAEAELKRLAEEIAGHDRRYHAEDAPTITDAEYDALRRRNLAIEERFPGLVREDSPSRRVGAPPAEGFA
KVRHAVPMLSLAKAYTDEDVTDFIERGRRFFDRDKDLDIAFTAEPKIDGLSASLRYENGVFVQGATRGDGAVGEDITANL
RTIADIPAKLKGSGWPDVIEIRGEVYMTYAEFEALKARSAAAGGQDYVNPRNTAAGSLRQKDASVTASRNLKFFAYAWGF
TTADPAPTQYESVQKFADWGFKISPLMVRAKSVEELVAQYHLIEEQRSSLGYDIDGVVYKVDQLELQRRWGFVTGEPRWA
VAHKFPAEQAMTTVQKIDIQVGRTGTLAPVARLAPVTVGGVVVENVTLHNEDYIKGFDSNGLPIRDGIDVRIGDTVVIQR
AGDVIPQIVSVVIDKRPADAVPYEFPHTCPVCGSPATREINEKTGKEDSRRRCTGELICAAQAVERLRHFVSRGALDIEG
LGAENIDTFFNAGLIKTAADIFTLRDRRPAVTKALAERREEQARQREAASGKTRKNVRSVEDRNYEGLDKLFAAIDSRRE
PELDRFIFALGIRHIGETTAAVLAKTFSTIEELIRVGKETAAAEDPHTVFPSVNGIGDTVIDALCDFFGNERNDDVLDKL
LEQVKPKPYIVTVSADSEVAGKTIVFTGTLEKMTRSEAKAMAERLGAKVAGSVSAKTDLLVAGPGAGSKLKLASELGVEV
IDEDTWLQRVGKA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=723, Percent_Identity=43.5684647302905, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI87082305, Length=477, Percent_Identity=25.5765199161426, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_RHILO (Q98KC4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103104.1
- HSSP:   O87703
- ProteinModelPortal:   Q98KC4
- GeneID:   1225767
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1539
- NMPDR:   fig|266835.1.peg.1209
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 80497; Mature: 80365

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 127-127 BINDING 125-125 BINDING 148-148 BINDING 185-185 BINDING 301-301 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKPVDSLSESEAEAELKRLAEEIAGHDRRYHAEDAPTITDAEYDALRRRNLAIEERFP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCC
GLVREDSPSRRVGAPPAEGFAKVRHAVPMLSLAKAYTDEDVTDFIERGRRFFDRDKDLDI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEE
AFTAEPKIDGLSASLRYENGVFVQGATRGDGAVGEDITANLRTIADIPAKLKGSGWPDVI
EEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCEE
EIRGEVYMTYAEFEALKARSAAAGGQDYVNPRNTAAGSLRQKDASVTASRNLKFFAYAWG
EECCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEC
FTTADPAPTQYESVQKFADWGFKISPLMVRAKSVEELVAQYHLIEEQRSSLGYDIDGVVY
CCCCCCCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
KVDQLELQRRWGFVTGEPRWAVAHKFPAEQAMTTVQKIDIQVGRTGTLAPVARLAPVTVG
EHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHEEECCCCCHHHHHHHCCCEEC
GVVVENVTLHNEDYIKGFDSNGLPIRDGIDVRIGDTVVIQRAGDVIPQIVSVVIDKRPAD
CEEEEEEEECCCHHHCCCCCCCCCCCCCCEEEECCEEEEEHHHHHHHHHHHHHHCCCCCC
AVPYEFPHTCPVCGSPATREINEKTGKEDSRRRCTGELICAAQAVERLRHFVSRGALDIE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
GLGAENIDTFFNAGLIKTAADIFTLRDRRPAVTKALAERREEQARQREAASGKTRKNVRS
CCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VEDRNYEGLDKLFAAIDSRREPELDRFIFALGIRHIGETTAAVLAKTFSTIEELIRVGKE
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TAAAEDPHTVFPSVNGIGDTVIDALCDFFGNERNDDVLDKLLEQVKPKPYIVTVSADSEV
HCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
AGKTIVFTGTLEKMTRSEAKAMAERLGAKVAGSVSAKTDLLVAGPGAGSKLKLASELGVE
CCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCEEEHHHCCCE
VIDEDTWLQRVGKA
EECCHHHHHHHCCC
>Mature Secondary Structure 
AEKPVDSLSESEAEAELKRLAEEIAGHDRRYHAEDAPTITDAEYDALRRRNLAIEERFP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCC
GLVREDSPSRRVGAPPAEGFAKVRHAVPMLSLAKAYTDEDVTDFIERGRRFFDRDKDLDI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEE
AFTAEPKIDGLSASLRYENGVFVQGATRGDGAVGEDITANLRTIADIPAKLKGSGWPDVI
EEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCEE
EIRGEVYMTYAEFEALKARSAAAGGQDYVNPRNTAAGSLRQKDASVTASRNLKFFAYAWG
EECCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEC
FTTADPAPTQYESVQKFADWGFKISPLMVRAKSVEELVAQYHLIEEQRSSLGYDIDGVVY
CCCCCCCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
KVDQLELQRRWGFVTGEPRWAVAHKFPAEQAMTTVQKIDIQVGRTGTLAPVARLAPVTVG
EHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHEEECCCCCHHHHHHHCCCEEC
GVVVENVTLHNEDYIKGFDSNGLPIRDGIDVRIGDTVVIQRAGDVIPQIVSVVIDKRPAD
CEEEEEEEECCCHHHCCCCCCCCCCCCCCEEEECCEEEEEHHHHHHHHHHHHHHCCCCCC
AVPYEFPHTCPVCGSPATREINEKTGKEDSRRRCTGELICAAQAVERLRHFVSRGALDIE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
GLGAENIDTFFNAGLIKTAADIFTLRDRRPAVTKALAERREEQARQREAASGKTRKNVRS
CCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VEDRNYEGLDKLFAAIDSRREPELDRFIFALGIRHIGETTAAVLAKTFSTIEELIRVGKE
HHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TAAAEDPHTVFPSVNGIGDTVIDALCDFFGNERNDDVLDKLLEQVKPKPYIVTVSADSEV
HCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
AGKTIVFTGTLEKMTRSEAKAMAERLGAKVAGSVSAKTDLLVAGPGAGSKLKLASELGVE
CCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCEEEHHHCCCE
VIDEDTWLQRVGKA
EECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968