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The map label for this gene is dhfrIII [H]

Identifier: 13471475

GI number: 13471475

Start: 1214550

End: 1215068

Strand: Reverse

Name: dhfrIII [H]

Synonym: mll1457

Alternate gene names: 13471475

Gene position: 1215068-1214550 (Counterclockwise)

Preceding gene: 13471476

Following gene: 13471474

Centisome position: 17.27

GC content: 66.09

Gene sequence:

>519_bases
ATGCATGTGGCAATCTACGTCGCCATCGCCGAGAACGGCGTGATCGGGCGGGACGGCGGACTGCCCTGGCGGCTTTCGAC
CGATCTCAAGCGCTTCAAGGCCGACACGATGGGCAAACCCATCATCATGGGCCGCAAGACTTATGAAGGCATCGGCCGGC
CGCTGCCGGGCAGGCTCAACTTCGTCGTCACCCGTGACAAGACCTGGCGCGCCGAGGGCGTCGAGGTGGCGCATACGCTG
GAGGCCGCGATTCAGCTGGCAACGGTGCGCGTCCGCTGCATGAGCGGCGTGGACGAGGCCTGCATCATCGGCGGCGGTGA
GATCTATGCCCAGGCGCTGCCGCTGGCCGACCGGCTGCACGTCACCCATGTGCTGGCCGCCGTCGACGGCGACGCGCATT
TCCCGCCGATCGACGCTGAGACATGGCGCATGGTCAGTTCGCAGGAGGTTCCGGCCGGGGAAAAGGACAGCCACGCAACG
CGCTATTCGGTTTACGAGCGCCGCCGCCAGATACTTTGA

Upstream 100 bases:

>100_bases
CGACAACTTTCGAGATGACCGCGAACGATTTTTACCCAGAGCACGACATCGATATCCTGCTGCTCCAACCGTCGGACAGT
AATGGCGGGGATGCGAACTG

Downstream 100 bases:

>100_bases
TAAACAAAACGTGCATTGAAGGACGACAAAGGGTCGCTACCGGCCCAAAACGGATGATGCCGGCGGCGCATCGCGTTGAA
AGCGTGCCGTGGCGTCCCTA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MHVAIYVAIAENGVIGRDGGLPWRLSTDLKRFKADTMGKPIIMGRKTYEGIGRPLPGRLNFVVTRDKTWRAEGVEVAHTL
EAAIQLATVRVRCMSGVDEACIIGGGEIYAQALPLADRLHVTHVLAAVDGDAHFPPIDAETWRMVSSQEVPAGEKDSHAT
RYSVYERRRQIL

Sequences:

>Translated_172_residues
MHVAIYVAIAENGVIGRDGGLPWRLSTDLKRFKADTMGKPIIMGRKTYEGIGRPLPGRLNFVVTRDKTWRAEGVEVAHTL
EAAIQLATVRVRCMSGVDEACIIGGGEIYAQALPLADRLHVTHVLAAVDGDAHFPPIDAETWRMVSSQEVPAGEKDSHAT
RYSVYERRRQIL
>Mature_172_residues
MHVAIYVAIAENGVIGRDGGLPWRLSTDLKRFKADTMGKPIIMGRKTYEGIGRPLPGRLNFVVTRDKTWRAEGVEVAHTL
EAAIQLATVRVRCMSGVDEACIIGGGEIYAQALPLADRLHVTHVLAAVDGDAHFPPIDAETWRMVSSQEVPAGEKDSHAT
RYSVYERRRQIL

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=181, Percent_Identity=30.939226519337, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1786233, Length=166, Percent_Identity=37.9518072289157, Blast_Score=123, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17505899, Length=161, Percent_Identity=32.2981366459627, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=172, Percent_Identity=29.0697674418605, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116008026, Length=176, Percent_Identity=30.1136363636364, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647458, Length=176, Percent_Identity=30.1136363636364, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18975; Mature: 18975

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVAIYVAIAENGVIGRDGGLPWRLSTDLKRFKADTMGKPIIMGRKTYEGIGRPLPGRLN
CEEEEEEEEECCCEEECCCCCCEEECHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCEE
FVVTRDKTWRAEGVEVAHTLEAAIQLATVRVRCMSGVDEACIIGGGEIYAQALPLADRLH
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCHHHHH
VTHVLAAVDGDAHFPPIDAETWRMVSSQEVPAGEKDSHATRYSVYERRRQIL
HHHHEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVAIYVAIAENGVIGRDGGLPWRLSTDLKRFKADTMGKPIIMGRKTYEGIGRPLPGRLN
CEEEEEEEEECCCEEECCCCCCEEECHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCEE
FVVTRDKTWRAEGVEVAHTLEAAIQLATVRVRCMSGVDEACIIGGGEIYAQALPLADRLH
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCHHHHH
VTHVLAAVDGDAHFPPIDAETWRMVSSQEVPAGEKDSHATRYSVYERRRQIL
HHHHEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]