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The map label for this gene is gcvT [H]

Identifier: 13471318

GI number: 13471318

Start: 1055064

End: 1057625

Strand: Reverse

Name: gcvT [H]

Synonym: mll1258

Alternate gene names: 13471318

Gene position: 1057625-1055064 (Counterclockwise)

Preceding gene: 13471319

Following gene: 13471317

Centisome position: 15.03

GC content: 62.33

Gene sequence:

>2562_bases
ATGGCAGAGTTTCCGAAAAAGGCGAAGGTCGTCATCATTGGCCTCGGCGGTATTGTCGGCGCATCGATCGCCCATCACCT
GATCGAGCGCGGATGGGACGATATTGTCGGCATCGACAAATCAGGTATCCCGACCGATATCGGCTCGACCGCGCACGCTT
CCGACTTCTGCTACACAACCAGCCATGATTTTCTGTCCTGCTGGACGACGCTTTATTCCATCGATTTCTACGAGAAGATG
GGCCATTACGCCCGCATCGGCGGCCTCGAAGTGGCTCGCGTCGGCGACGACAGCCGCATGGACGAGATCAAGCGCAAGAT
CGCCTCGGCAAAGGCCTTTGGCACACGCGCGCGCCTGATCGAACCGGCCGAGATCAAGGAGAAATTCCCGCTGATCGAAG
AGGGGATGGTGCAGGGCGGCCTTTGGGATCCGGACGCAGGTCTTGTCATCCCGCGCTCGCAGACCGTTGCCGGCAAGCTG
GTCGACCAGGCTGAAGCGTCAGGCAAGCTCAAATCCTTCGCCAACACGCCGGCCAGGTCGCTCGTCGTCAAGGACGGCCG
CATCAGCGCCGTGGTGACCGACCGCGGCACCATCGAGGCCGATTATGTCATCGTCTGCGCCGGCATCTGGGGTCGGTTGA
TCGCCGAAATGGTCGGCGAGGACCTGCCGGTTATGCCGATCGACCATCCGCTGACGTTCTTCGGGCCCTACAATGAGTTC
GCCGGCACCGGCAAGGAGATCGGCTGGCCGCTGCTGCGCGACCAGGGCAACTCGGCCTATATGCGTGACACCGGCGACCC
CAAGACCGCCGAGGGCGGGCAGATCGAATGGGGCTATTACGAAGAAAACAACCCGCGCCTTTGCCATCCGCGCGATCTCC
TTGAAAAGCATGAAGCGCGTTTGTCGCCGTCGCAGCGCGACCTCGACATGGAGCAGATCCTGGCGCCGCTGGAACGCGCC
ATGGAGCTGACGCCGATCCTGGGCGAACTCGGCTACAATGAAAGCCATTCCTTCAACGGCCTGCTGCAGGTCACGGCCGA
CGGTGGCCCGTCCATGGGCGAAAGCCAGAAGGTGCGGGGGCTCTGGTATGCCGTCGCCATCTGGGTCAAGGACGGCCCCG
GCATGGGCAAGCTGATCGCCGACTGGATGACGGATGGCCGCACGGCGATCGACCATCACGCCATCGACTATGCGCGCTTC
TATCCGCACCAGACGAAGGAGCAGTTCATCTGGGATCGCTGCACCGAGACGGCGATGAAGGTCTACAATCCCGCGGTGCA
TCCGCGCGAGCCCTTCTCCAAGGGCCGCAACATAAGGCGTTCGCCGTTCTGGGAGCGCGAGAAGGAACTCGGCGGCTATT
TCATGGAACTGGGCGGCTGGGAGCGTGCCCATGGCTATGCCGCCAACGAGCACCTGCTCGAGAAATATGGCAACCGCGTT
CCCGTGCGCGAGAACGAGTGGGACAACCGCCATTTCTGGCGCGTCTCCAATGCCGAGCATCTGGCGATGAGCGAGGATTG
CGGCATCGTCAACCTGTCGCATTTCTCTATGTATGACGTCGAAGGGCCAGATCACGTCGCGCTGCTGGAATGGCTGTGCG
CGGCCAAGATCGGCGGCGACAACAACATCGGCAAGGGCATCTACACCCACTTCCTCGACGAGGAAGGCATGGTGCGCGCC
GACTTCACCGTGATCCGCATGGCCGATCGCTGCCGAGTGATCGACGGCGCCGACGCCGGCCCGCGCGACTTCCGCTATAT
GCAGCGCACCGCGCAGGACAAAGGTTTTGACGCCACCATCACCGATGTGACGGAGAAATACGTCACCATCGGCATCTGGG
GTCCGAATGCCCGGACCACCTTGAAGAAGGTGGTCGAGAATCCCGAGGGCCTTTCGCCGGAGAATTTCCCCTTCGCGGCG
ATCAAGCCGGTCAGGATCGGCGGCAAGGATGTCACCGCTTTCCGCATCTCCTATGTCGGCGAGCAGGGCTGGGAACTGCA
TATGCGCTATGAGGACGGCCTGGCCGTCTGGGACGCGCTGCGCTCGACCGGCGTCATGCCGTTCGGCGTCGAGACCTATG
CCAACACGCGCCGCATGGAAAAGAGCCTGCGCCTGCAGAATGCCGACCTTCTGACCGAGTACAATCTGCTGGAAGCCGAT
CTCGCCCGACCGAAGGTCAAAGAGAACGATTTCTGCGGCAAGGCCAAGCATGTTGAATACCGCGCGCGCGAGCACCAGCC
GGCGACTTTGTGCACGCTGGTGATGACCGAAAACACCGATTCCAAGGGCGTGGCGCGTTATCCCGTTGGCACCATGCCGG
TGCTGGACCCGGCCACCGGGGAAACGCTGGTCGACGAGCTCGGCCGCCGCTCCTTCACCACCTCGGTCGCCTATGGCCCG
ACGATCGGCAAGAACATCGCGCTCGCCTATCTGCCGCATTCGTATGCGCAGGAAGGTCGCAAGCTCAATGTCGAGTATTT
CGGCGAGACCTATCCGGTCGAAGTCGCCGGTGTCGGCTACAAGCCGCTTTATGACCCGGAGAACCTCAAGCCGCGCAGCT
GA

Upstream 100 bases:

>100_bases
TGTCGCAAACAGGCGGTAGCGCCGTGCCTGTCGTCTGCATATTGTGCGCCGATCAAACGGGCGCCGGCGCTGCCCGCAAT
AGCATCTGGAGTCGCGGGCA

Downstream 100 bases:

>100_bases
TCGGTCGGGCGGACCACGGGACTTATTGCAAAAGCCTGGCTTCGAGCCGGGCTTTTGTCTTTTTAAGCAGGTGGTTTTCG
CTTACCCTCGCGGCATGTCT

Product: sarcosine dehydrogenase

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 853; Mature: 852

Protein sequence:

>853_residues
MAEFPKKAKVVIIGLGGIVGASIAHHLIERGWDDIVGIDKSGIPTDIGSTAHASDFCYTTSHDFLSCWTTLYSIDFYEKM
GHYARIGGLEVARVGDDSRMDEIKRKIASAKAFGTRARLIEPAEIKEKFPLIEEGMVQGGLWDPDAGLVIPRSQTVAGKL
VDQAEASGKLKSFANTPARSLVVKDGRISAVVTDRGTIEADYVIVCAGIWGRLIAEMVGEDLPVMPIDHPLTFFGPYNEF
AGTGKEIGWPLLRDQGNSAYMRDTGDPKTAEGGQIEWGYYEENNPRLCHPRDLLEKHEARLSPSQRDLDMEQILAPLERA
MELTPILGELGYNESHSFNGLLQVTADGGPSMGESQKVRGLWYAVAIWVKDGPGMGKLIADWMTDGRTAIDHHAIDYARF
YPHQTKEQFIWDRCTETAMKVYNPAVHPREPFSKGRNIRRSPFWEREKELGGYFMELGGWERAHGYAANEHLLEKYGNRV
PVRENEWDNRHFWRVSNAEHLAMSEDCGIVNLSHFSMYDVEGPDHVALLEWLCAAKIGGDNNIGKGIYTHFLDEEGMVRA
DFTVIRMADRCRVIDGADAGPRDFRYMQRTAQDKGFDATITDVTEKYVTIGIWGPNARTTLKKVVENPEGLSPENFPFAA
IKPVRIGGKDVTAFRISYVGEQGWELHMRYEDGLAVWDALRSTGVMPFGVETYANTRRMEKSLRLQNADLLTEYNLLEAD
LARPKVKENDFCGKAKHVEYRAREHQPATLCTLVMTENTDSKGVARYPVGTMPVLDPATGETLVDELGRRSFTTSVAYGP
TIGKNIALAYLPHSYAQEGRKLNVEYFGETYPVEVAGVGYKPLYDPENLKPRS

Sequences:

>Translated_853_residues
MAEFPKKAKVVIIGLGGIVGASIAHHLIERGWDDIVGIDKSGIPTDIGSTAHASDFCYTTSHDFLSCWTTLYSIDFYEKM
GHYARIGGLEVARVGDDSRMDEIKRKIASAKAFGTRARLIEPAEIKEKFPLIEEGMVQGGLWDPDAGLVIPRSQTVAGKL
VDQAEASGKLKSFANTPARSLVVKDGRISAVVTDRGTIEADYVIVCAGIWGRLIAEMVGEDLPVMPIDHPLTFFGPYNEF
AGTGKEIGWPLLRDQGNSAYMRDTGDPKTAEGGQIEWGYYEENNPRLCHPRDLLEKHEARLSPSQRDLDMEQILAPLERA
MELTPILGELGYNESHSFNGLLQVTADGGPSMGESQKVRGLWYAVAIWVKDGPGMGKLIADWMTDGRTAIDHHAIDYARF
YPHQTKEQFIWDRCTETAMKVYNPAVHPREPFSKGRNIRRSPFWEREKELGGYFMELGGWERAHGYAANEHLLEKYGNRV
PVRENEWDNRHFWRVSNAEHLAMSEDCGIVNLSHFSMYDVEGPDHVALLEWLCAAKIGGDNNIGKGIYTHFLDEEGMVRA
DFTVIRMADRCRVIDGADAGPRDFRYMQRTAQDKGFDATITDVTEKYVTIGIWGPNARTTLKKVVENPEGLSPENFPFAA
IKPVRIGGKDVTAFRISYVGEQGWELHMRYEDGLAVWDALRSTGVMPFGVETYANTRRMEKSLRLQNADLLTEYNLLEAD
LARPKVKENDFCGKAKHVEYRAREHQPATLCTLVMTENTDSKGVARYPVGTMPVLDPATGETLVDELGRRSFTTSVAYGP
TIGKNIALAYLPHSYAQEGRKLNVEYFGETYPVEVAGVGYKPLYDPENLKPRS
>Mature_852_residues
AEFPKKAKVVIIGLGGIVGASIAHHLIERGWDDIVGIDKSGIPTDIGSTAHASDFCYTTSHDFLSCWTTLYSIDFYEKMG
HYARIGGLEVARVGDDSRMDEIKRKIASAKAFGTRARLIEPAEIKEKFPLIEEGMVQGGLWDPDAGLVIPRSQTVAGKLV
DQAEASGKLKSFANTPARSLVVKDGRISAVVTDRGTIEADYVIVCAGIWGRLIAEMVGEDLPVMPIDHPLTFFGPYNEFA
GTGKEIGWPLLRDQGNSAYMRDTGDPKTAEGGQIEWGYYEENNPRLCHPRDLLEKHEARLSPSQRDLDMEQILAPLERAM
ELTPILGELGYNESHSFNGLLQVTADGGPSMGESQKVRGLWYAVAIWVKDGPGMGKLIADWMTDGRTAIDHHAIDYARFY
PHQTKEQFIWDRCTETAMKVYNPAVHPREPFSKGRNIRRSPFWEREKELGGYFMELGGWERAHGYAANEHLLEKYGNRVP
VRENEWDNRHFWRVSNAEHLAMSEDCGIVNLSHFSMYDVEGPDHVALLEWLCAAKIGGDNNIGKGIYTHFLDEEGMVRAD
FTVIRMADRCRVIDGADAGPRDFRYMQRTAQDKGFDATITDVTEKYVTIGIWGPNARTTLKKVVENPEGLSPENFPFAAI
KPVRIGGKDVTAFRISYVGEQGWELHMRYEDGLAVWDALRSTGVMPFGVETYANTRRMEKSLRLQNADLLTEYNLLEADL
ARPKVKENDFCGKAKHVEYRAREHQPATLCTLVMTENTDSKGVARYPVGTMPVLDPATGETLVDELGRRSFTTSVAYGPT
IGKNIALAYLPHSYAQEGRKLNVEYFGETYPVEVAGVGYKPLYDPENLKPRS

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI24797151, Length=855, Percent_Identity=28.187134502924, Blast_Score=273, Evalue=6e-73,
Organism=Homo sapiens, GI197927446, Length=778, Percent_Identity=26.0925449871465, Blast_Score=206, Evalue=7e-53,
Organism=Homo sapiens, GI21361378, Length=778, Percent_Identity=26.0925449871465, Blast_Score=206, Evalue=7e-53,
Organism=Homo sapiens, GI194306651, Length=753, Percent_Identity=25.6308100929615, Blast_Score=177, Evalue=3e-44,
Organism=Escherichia coli, GI1789272, Length=354, Percent_Identity=26.8361581920904, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32563613, Length=831, Percent_Identity=23.5860409145608, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71994045, Length=834, Percent_Identity=22.9016786570743, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71994052, Length=841, Percent_Identity=22.5921521997622, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI28571104, Length=765, Percent_Identity=25.8823529411765, Blast_Score=194, Evalue=3e-49,
Organism=Drosophila melanogaster, GI20130091, Length=897, Percent_Identity=24.1917502787068, Blast_Score=175, Evalue=1e-43,
Organism=Drosophila melanogaster, GI20129441, Length=350, Percent_Identity=24.2857142857143, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 95352; Mature: 95221

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFPKKAKVVIIGLGGIVGASIAHHLIERGWDDIVGIDKSGIPTDIGSTAHASDFCYTT
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEC
SHDFLSCWTTLYSIDFYEKMGHYARIGGLEVARVGDDSRMDEIKRKIASAKAFGTRARLI
CHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHCC
EPAEIKEKFPLIEEGMVQGGLWDPDAGLVIPRSQTVAGKLVDQAEASGKLKSFANTPARS
CCHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHCCCCCE
LVVKDGRISAVVTDRGTIEADYVIVCAGIWGRLIAEMVGEDLPVMPIDHPLTFFGPYNEF
EEEECCCEEEEEECCCCEEECEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHH
AGTGKEIGWPLLRDQGNSAYMRDTGDPKTAEGGQIEWGYYEENNPRLCHPRDLLEKHEAR
CCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCEEEECEEECCCCCEECHHHHHHHHHHH
LSPSQRDLDMEQILAPLERAMELTPILGELGYNESHSFNGLLQVTADGGPSMGESQKVRG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHCE
LWYAVAIWVKDGPGMGKLIADWMTDGRTAIDHHAIDYARFYPHQTKEQFIWDRCTETAMK
EEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VYNPAVHPREPFSKGRNIRRSPFWEREKELGGYFMELGGWERAHGYAANEHLLEKYGNRV
HHCCCCCCCCHHHHCCCCCCCCCHHHHHHHCCEEEECCCCHHHCCCCHHHHHHHHHCCCC
PVRENEWDNRHFWRVSNAEHLAMSEDCGIVNLSHFSMYDVEGPDHVALLEWLCAAKIGGD
CCCCCCCCCCEEEEECCCCEEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHCCCC
NNIGKGIYTHFLDEEGMVRADFTVIRMADRCRVIDGADAGPRDFRYMQRTAQDKGFDATI
CCCCCHHHHHHCCCCCCEEECEEEEEEHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCH
TDVTEKYVTIGIWGPNARTTLKKVVENPEGLSPENFPFAAIKPVRIGGKDVTAFRISYVG
HHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEEEC
EQGWELHMRYEDGLAVWDALRSTGVMPFGVETYANTRRMEKSLRLQNADLLTEYNLLEAD
CCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHH
LARPKVKENDFCGKAKHVEYRAREHQPATLCTLVMTENTDSKGVARYPVGTMPVLDPATG
HCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCC
ETLVDELGRRSFTTSVAYGPTIGKNIALAYLPHSYAQEGRKLNVEYFGETYPVEVAGVGY
HHHHHHHCCCCEECEEEECCCCCCCEEEEECCCHHHHCCCEEEEEECCCCCCEEEECCCC
KPLYDPENLKPRS
CCCCCCCCCCCCC
>Mature Secondary Structure 
AEFPKKAKVVIIGLGGIVGASIAHHLIERGWDDIVGIDKSGIPTDIGSTAHASDFCYTT
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEC
SHDFLSCWTTLYSIDFYEKMGHYARIGGLEVARVGDDSRMDEIKRKIASAKAFGTRARLI
CHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHCC
EPAEIKEKFPLIEEGMVQGGLWDPDAGLVIPRSQTVAGKLVDQAEASGKLKSFANTPARS
CCHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHCCCCCE
LVVKDGRISAVVTDRGTIEADYVIVCAGIWGRLIAEMVGEDLPVMPIDHPLTFFGPYNEF
EEEECCCEEEEEECCCCEEECEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHH
AGTGKEIGWPLLRDQGNSAYMRDTGDPKTAEGGQIEWGYYEENNPRLCHPRDLLEKHEAR
CCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCEEEECEEECCCCCEECHHHHHHHHHHH
LSPSQRDLDMEQILAPLERAMELTPILGELGYNESHSFNGLLQVTADGGPSMGESQKVRG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHCE
LWYAVAIWVKDGPGMGKLIADWMTDGRTAIDHHAIDYARFYPHQTKEQFIWDRCTETAMK
EEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VYNPAVHPREPFSKGRNIRRSPFWEREKELGGYFMELGGWERAHGYAANEHLLEKYGNRV
HHCCCCCCCCHHHHCCCCCCCCCHHHHHHHCCEEEECCCCHHHCCCCHHHHHHHHHCCCC
PVRENEWDNRHFWRVSNAEHLAMSEDCGIVNLSHFSMYDVEGPDHVALLEWLCAAKIGGD
CCCCCCCCCCEEEEECCCCEEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHCCCC
NNIGKGIYTHFLDEEGMVRADFTVIRMADRCRVIDGADAGPRDFRYMQRTAQDKGFDATI
CCCCCHHHHHHCCCCCCEEECEEEEEEHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCH
TDVTEKYVTIGIWGPNARTTLKKVVENPEGLSPENFPFAAIKPVRIGGKDVTAFRISYVG
HHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEEEC
EQGWELHMRYEDGLAVWDALRSTGVMPFGVETYANTRRMEKSLRLQNADLLTEYNLLEAD
CCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHH
LARPKVKENDFCGKAKHVEYRAREHQPATLCTLVMTENTDSKGVARYPVGTMPVLDPATG
HCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCC
ETLVDELGRRSFTTSVAYGPTIGKNIALAYLPHSYAQEGRKLNVEYFGETYPVEVAGVGY
HHHHHHHCCCCEECEEEECCCCCCCEEEEECCCHHHHCCCEEEEEECCCCCCEEEECCCC
KPLYDPENLKPRS
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA