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The map label for this gene is metH [H]

Identifier: 13471306

GI number: 13471306

Start: 1043183

End: 1044157

Strand: Direct

Name: metH [H]

Synonym: mlr1243

Alternate gene names: 13471306

Gene position: 1043183-1044157 (Clockwise)

Preceding gene: 13471305

Following gene: 13471308

Centisome position: 14.83

GC content: 65.23

Gene sequence:

>975_bases
ATGACCCGGACCATCGTTGCCTCGGCGACACGAGAAATCATCATCGGCTTCGACCAGCCCTTCTGCGTGATTGGCGAGCG
CATCAACCCGACCGGCCGCAAAAAGCTCGCCGCCGAGATGGTGGCCGGCAATTTCGAGACCGTCATCAAGGACGCGCTGG
AACAGGCCGCCTGCGGCGCCACCATGCTCGACGTCAATGCCGGCGTCACCGCCGTCGACCCCAACGCCACCGAGCCGGCG
CTGCTGGTGCAGACGCTGGAGATCGTCCAGGGCCTCGTCGACCTGCCGCTGTCGATCGATAGTTCGGTCACCGCCGCGAT
CGAGGCGGCGCTCAAGGTCGCCAAGGGCCGCCCGTTGGTCAATTCCGTCACTGGCGAGGAAGAAAAACTCGAGGCCATCC
TCCCGCTGATCAAGAAATACAACGTCCCGGTCGTCGCCATCTCCAATGACGAGACCGGCATTTCGATGGATCCGGACGTG
CGCTTCGCCGTCGCCAAGAAGATCGTCCAGCGCTGCGCCGATTTCGGCATTCCAGCGCACGACGTCGTCGTCGACCCGCT
GGTCATGCCGATCGGCGCGCTGGGTGACGCTGGCCGCCAGGTGTTCGCGTTGCTGCGTCGGCTCCGCGAAGAGCTCAAGG
TCAACACCACCTGCGGCCTGTCCAACATCTCGTTCGGCCTGCCGCATCGCCATGGCATCAACGCCGCCTTCATCCCGATG
GTGATCGGCGCCGGCATGACATCAGCCATCATGAACCCGGTGCGTCCGCAGGAAATGGAAGCCGTGCGGGGCGCCAATGT
GCTCAACGGCACCGACGAGAATTGCACCAACTGGATCAGGACCTACAAGGACTACAAGCCGGCCGAAGGCGGCCATGCTG
TCGCGGCGCCGACGCAGGTCAATGCGCCTGGCGATGGCGCGGCCAACGGTGGCCGCCGCCGCGGCGGCCGTGAAGCCCGT
ATGGGTCGGGGGTAA

Upstream 100 bases:

>100_bases
GGTGAAGACCGCGCATTGATCCGCGCGGAGGACCACCATTCGCTTGATCGAAAAGAGATAACGATATAAAGAACTCTTTA
TATCACGACGGAGAGATTTC

Downstream 100 bases:

>100_bases
GCGCGCAATCGTGAACCGGACTGCAAACCACGCTGGACCGATCGCATCATCGATGCGGTCGGAGGCGTCTGCGTTGCGGT
CAGGCGATACCCAATATGGC

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTRTIVASATREIIIGFDQPFCVIGERINPTGRKKLAAEMVAGNFETVIKDALEQAACGATMLDVNAGVTAVDPNATEPA
LLVQTLEIVQGLVDLPLSIDSSVTAAIEAALKVAKGRPLVNSVTGEEEKLEAILPLIKKYNVPVVAISNDETGISMDPDV
RFAVAKKIVQRCADFGIPAHDVVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAAFIPM
VIGAGMTSAIMNPVRPQEMEAVRGANVLNGTDENCTNWIRTYKDYKPAEGGHAVAAPTQVNAPGDGAANGGRRRGGREAR
MGRG

Sequences:

>Translated_324_residues
MTRTIVASATREIIIGFDQPFCVIGERINPTGRKKLAAEMVAGNFETVIKDALEQAACGATMLDVNAGVTAVDPNATEPA
LLVQTLEIVQGLVDLPLSIDSSVTAAIEAALKVAKGRPLVNSVTGEEEKLEAILPLIKKYNVPVVAISNDETGISMDPDV
RFAVAKKIVQRCADFGIPAHDVVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAAFIPM
VIGAGMTSAIMNPVRPQEMEAVRGANVLNGTDENCTNWIRTYKDYKPAEGGHAVAAPTQVNAPGDGAANGGRRRGGREAR
MGRG
>Mature_323_residues
TRTIVASATREIIIGFDQPFCVIGERINPTGRKKLAAEMVAGNFETVIKDALEQAACGATMLDVNAGVTAVDPNATEPAL
LVQTLEIVQGLVDLPLSIDSSVTAAIEAALKVAKGRPLVNSVTGEEEKLEAILPLIKKYNVPVVAISNDETGISMDPDVR
FAVAKKIVQRCADFGIPAHDVVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAAFIPMV
IGAGMTSAIMNPVRPQEMEAVRGANVLNGTDENCTNWIRTYKDYKPAEGGHAVAAPTQVNAPGDGAANGGRRRGGREARM
GRG

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=249, Percent_Identity=30.9236947791165, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790450, Length=254, Percent_Identity=26.3779527559055, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=254, Percent_Identity=28.3464566929134, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 34280; Mature: 34149

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTIVASATREIIIGFDQPFCVIGERINPTGRKKLAAEMVAGNFETVIKDALEQAACGA
CCCEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
TMLDVNAGVTAVDPNATEPALLVQTLEIVQGLVDLPLSIDSSVTAAIEAALKVAKGRPLV
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCH
NSVTGEEEKLEAILPLIKKYNVPVVAISNDETGISMDPDVRFAVAKKIVQRCADFGIPAH
HCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
DVVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAAFIPM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VIGAGMTSAIMNPVRPQEMEAVRGANVLNGTDENCTNWIRTYKDYKPAEGGHAVAAPTQV
HHHCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
NAPGDGAANGGRRRGGREARMGRG
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRTIVASATREIIIGFDQPFCVIGERINPTGRKKLAAEMVAGNFETVIKDALEQAACGA
CCEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
TMLDVNAGVTAVDPNATEPALLVQTLEIVQGLVDLPLSIDSSVTAAIEAALKVAKGRPLV
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCH
NSVTGEEEKLEAILPLIKKYNVPVVAISNDETGISMDPDVRFAVAKKIVQRCADFGIPAH
HCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
DVVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAAFIPM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VIGAGMTSAIMNPVRPQEMEAVRGANVLNGTDENCTNWIRTYKDYKPAEGGHAVAAPTQV
HHHCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
NAPGDGAANGGRRRGGREARMGRG
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]