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The map label for this gene is dapE [H]

Identifier: 13471265

GI number: 13471265

Start: 996669

End: 997946

Strand: Direct

Name: dapE [H]

Synonym: mlr1185

Alternate gene names: 13471265

Gene position: 996669-997946 (Clockwise)

Preceding gene: 13471261

Following gene: 13471266

Centisome position: 14.17

GC content: 63.85

Gene sequence:

>1278_bases
ATGAACGAGCGACTCCTTCAAGCAATCGATGACAGGCGCGACGATGTTGTCGCGCTGACCGCTGACCTGATCCGTTTCCC
GACCATCAATCCGCCTGGCGAAGCCTACCGGCCATGTGCGGAATTTGTCGGCGCGCGGCTGAGGAAACGTGGTTTTGAAA
CCGAATTCATCCGTGCCGAAGGCACGCCCGGCGACACCGACCGCTACCCGCGCGTCAATGTCGTGGCCCGTTTCGACGGC
CGATCGCCCGGCGCCTGCGTCCACTTCAACTCGCATATCGACGTGGTCGAGGCCGGCGACGGCTGGACCGTCGATCCCTT
TGCCGGCATCGTCAGGGACGGCAGGGTTTATGGACGCGGCGCCTGCGACATGAAGGGCGGCCTGGCCGCGTCGATCATCG
CCGCCGAGGCGTTCATGGAGGTCTATCCCGACTTTCCCGGCACCATCGAGATATCCGGCACCGTCGACGAGGAGTCCGGC
GGCTTTGGCGGTGTCGCGCATCTGGCCGGGCTCGGCTATTTCTCCAAACCCAGGGTCGACCACGTCATCATCCCCGAACC
GCTGAACAAGGACCGCATCTGCCTTGGCCATCGCGGCGTCTGGTGGGCCGAGATCGAAACCAAGGGCGAAATCGCGCACG
GCTCGATGCCGTTTCTCGGCGACAATGCGGTGCGCCATATGGGCGCCGTGCTGCAAGCTTTCGAGGACGAACTGTTCCCG
GCACTCGACCGCAAGATGACGCGCATGCCGGTGGTGCCCGAAGGCGCCAGGCGCTCGACCATGAACATCAATTCCATCCA
TGGCGGCCAGACCGAAGATTTCCGACCCGGCCTGCCCTCGCCCAACGTGCCCGATTCCTGCCGGCTGACCATCGACCGCC
GCTTCCTGCTCGAGGAAGACCTCGCCACGGTCAAAGGCGAAGTGACCGGCATTCTCGAGCGGCTGAAGCGCGAGCGCAAA
AAGTTCGATTACGAGATCCGCGACCTGATGGAAGTGCTGCCGCTGATGACCGAGCGCGACGCGCCGGTGGTCAAGGCCGT
GGCGCAAGGCATTCATGCGATCTTCGACCGCGAGCCCGACTACGTCATTTCGCCCGGCACCTACGACCAGAAGCACATTG
CCCGCATCGGCCACATCTACGACTGCATCGCCTATGGGCCCGGCATTCTCGACCTCGCCCACCGGCCAGACGAATGGGTT
GGCATAGAGGACATGGTGGAATCGGCCAAGGTGATGGCGATCGGCCTCAACGTGCTGCTGCGCGGCACGACCGGATGA

Upstream 100 bases:

>100_bases
TTTGAACGATTTCCCGCGATAGTTGCCGCAACGACACTCCGCACCGGCTTGATTGCCGGCGGGCTTGCAATGCAACGCCG
TTCGTTGCTCAACTCCCCCG

Downstream 100 bases:

>100_bases
CCGGCCCGCGGCGCAAAAACATTTCTCCTGGCGCGGCGCGACAGCACGAAACGCAATTTACTCCCGAGCGAAATCACGCT
TCTATCCCCGGGCTTGAGCA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNERLLQAIDDRRDDVVALTADLIRFPTINPPGEAYRPCAEFVGARLRKRGFETEFIRAEGTPGDTDRYPRVNVVARFDG
RSPGACVHFNSHIDVVEAGDGWTVDPFAGIVRDGRVYGRGACDMKGGLAASIIAAEAFMEVYPDFPGTIEISGTVDEESG
GFGGVAHLAGLGYFSKPRVDHVIIPEPLNKDRICLGHRGVWWAEIETKGEIAHGSMPFLGDNAVRHMGAVLQAFEDELFP
ALDRKMTRMPVVPEGARRSTMNINSIHGGQTEDFRPGLPSPNVPDSCRLTIDRRFLLEEDLATVKGEVTGILERLKRERK
KFDYEIRDLMEVLPLMTERDAPVVKAVAQGIHAIFDREPDYVISPGTYDQKHIARIGHIYDCIAYGPGILDLAHRPDEWV
GIEDMVESAKVMAIGLNVLLRGTTG

Sequences:

>Translated_425_residues
MNERLLQAIDDRRDDVVALTADLIRFPTINPPGEAYRPCAEFVGARLRKRGFETEFIRAEGTPGDTDRYPRVNVVARFDG
RSPGACVHFNSHIDVVEAGDGWTVDPFAGIVRDGRVYGRGACDMKGGLAASIIAAEAFMEVYPDFPGTIEISGTVDEESG
GFGGVAHLAGLGYFSKPRVDHVIIPEPLNKDRICLGHRGVWWAEIETKGEIAHGSMPFLGDNAVRHMGAVLQAFEDELFP
ALDRKMTRMPVVPEGARRSTMNINSIHGGQTEDFRPGLPSPNVPDSCRLTIDRRFLLEEDLATVKGEVTGILERLKRERK
KFDYEIRDLMEVLPLMTERDAPVVKAVAQGIHAIFDREPDYVISPGTYDQKHIARIGHIYDCIAYGPGILDLAHRPDEWV
GIEDMVESAKVMAIGLNVLLRGTTG
>Mature_425_residues
MNERLLQAIDDRRDDVVALTADLIRFPTINPPGEAYRPCAEFVGARLRKRGFETEFIRAEGTPGDTDRYPRVNVVARFDG
RSPGACVHFNSHIDVVEAGDGWTVDPFAGIVRDGRVYGRGACDMKGGLAASIIAAEAFMEVYPDFPGTIEISGTVDEESG
GFGGVAHLAGLGYFSKPRVDHVIIPEPLNKDRICLGHRGVWWAEIETKGEIAHGSMPFLGDNAVRHMGAVLQAFEDELFP
ALDRKMTRMPVVPEGARRSTMNINSIHGGQTEDFRPGLPSPNVPDSCRLTIDRRFLLEEDLATVKGEVTGILERLKRERK
KFDYEIRDLMEVLPLMTERDAPVVKAVAQGIHAIFDREPDYVISPGTYDQKHIARIGHIYDCIAYGPGILDLAHRPDEWV
GIEDMVESAKVMAIGLNVLLRGTTG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=403, Percent_Identity=26.7990074441687, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789236, Length=343, Percent_Identity=24.4897959183673, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790395, Length=362, Percent_Identity=26.2430939226519, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356353, Length=319, Percent_Identity=26.6457680250784, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24649206, Length=307, Percent_Identity=22.1498371335505, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 46946; Mature: 46946

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERLLQAIDDRRDDVVALTADLIRFPTINPPGEAYRPCAEFVGARLRKRGFETEFIRAE
CCHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
GTPGDTDRYPRVNVVARFDGRSPGACVHFNSHIDVVEAGDGWTVDPFAGIVRDGRVYGRG
CCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEECCCCEEECHHHHHHHCCEEECCC
ACDMKGGLAASIIAAEAFMEVYPDFPGTIEISGTVDEESGGFGGVAHLAGLGYFSKPRVD
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCC
HVIIPEPLNKDRICLGHRGVWWAEIETKGEIAHGSMPFLGDNAVRHMGAVLQAFEDELFP
EEECCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALDRKMTRMPVVPEGARRSTMNINSIHGGQTEDFRPGLPSPNVPDSCRLTIDRRFLLEED
HHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
LATVKGEVTGILERLKRERKKFDYEIRDLMEVLPLMTERDAPVVKAVAQGIHAIFDREPD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
YVISPGTYDQKHIARIGHIYDCIAYGPGILDLAHRPDEWVGIEDMVESAKVMAIGLNVLL
EEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHEEEEEEEEEE
RGTTG
ECCCC
>Mature Secondary Structure
MNERLLQAIDDRRDDVVALTADLIRFPTINPPGEAYRPCAEFVGARLRKRGFETEFIRAE
CCHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
GTPGDTDRYPRVNVVARFDGRSPGACVHFNSHIDVVEAGDGWTVDPFAGIVRDGRVYGRG
CCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEECCCCEEECHHHHHHHCCEEECCC
ACDMKGGLAASIIAAEAFMEVYPDFPGTIEISGTVDEESGGFGGVAHLAGLGYFSKPRVD
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCC
HVIIPEPLNKDRICLGHRGVWWAEIETKGEIAHGSMPFLGDNAVRHMGAVLQAFEDELFP
EEECCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALDRKMTRMPVVPEGARRSTMNINSIHGGQTEDFRPGLPSPNVPDSCRLTIDRRFLLEED
HHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
LATVKGEVTGILERLKRERKKFDYEIRDLMEVLPLMTERDAPVVKAVAQGIHAIFDREPD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
YVISPGTYDQKHIARIGHIYDCIAYGPGILDLAHRPDEWVGIEDMVESAKVMAIGLNVLL
EEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHEEEEEEEEEE
RGTTG
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]