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The map label for this gene is rpfC [H]

Identifier: 13471220

GI number: 13471220

Start: 948061

End: 949401

Strand: Direct

Name: rpfC [H]

Synonym: mlr1132

Alternate gene names: 13471220

Gene position: 948061-949401 (Clockwise)

Preceding gene: 13471219

Following gene: 13471222

Centisome position: 13.47

GC content: 66.67

Gene sequence:

>1341_bases
ATGCCGCTCAGGGACATAAACGATCTCGAAAAACTGAAGAAGATCAACGCCGCGCTGGTCGACCGCGTCGAGCGGTCGAT
GGACCAGCAGGGCAACGCCTTCTCGCTGTTCCAGACAGCCATCTCGCTGGAAAATCGGGTGCGCACGCGCACCGAGGAGC
TGCACTCGACCTTGAGGCGGCTGGAACAATCCAACATCGATCTCAGCGCCGCCAAGGAAAACGCCGAGTTCGCGAACCTG
TCCAAGACGAGGTTCCTCGCCGCCGCCAGCCACGACGTGCTGCAGCCGCTGAATGCGGCTCACCTATCCGTCTCGGCGCT
CGCCGAGTTGCAGACCAGCGACGAAGGCAGGAAGCTGGTGCGGCAGGTCGAGCGCTCGCTGGAGACGATGGAGGACCTGC
TGCGCACCTTGCTCGACATTTCCAAGCTCGATGCCGGCGTGGTGCACCCCGACATCGGCGACGTCAGCCTGGAGGCGCTG
TTCTCGTCGCTGCGCTCGGATTTCCAGCCGGTCGCGGAAATGAAGCGGCTGTCCTTGAAATTTCGGCCGGTCAACGCCGT
GGTCCGCTCCGACCGGACGCTGCTGCGCCGCATCCTGCAGAACATCCTCTCCAACGCGCTGGGCTATACCCGCTCGGGCG
GCGTCCTGGTCGGCACCAGGCATCGCGGCGACACGATCCGCATCGATGTCGCCGATACCGGCTGCGGCATTCCCGAGGAC
CAGCGCGAGGCGGTGTTCGAGGAATTCCATCGCGGCACCTTGCCCGCCAATGCCGGGCTCGACGGCGGCGGCCTCGGGCT
CGGGCTTGCCATCGTGCGCCGCATGGCCGGCGCGCTCGGCCACCCCGTGACCTTCTCCTCGAAGGTCGGGCGCGGCACGA
TCTTTCATATCGATGTCCCCGTCGGCATCGGCGCCCCCGCCGACGCCATCGCCAGCGCCACCAGCCTGGAGCGGTCGCGC
GGCTACGGCCTGTTCGGCACCAAGGTGTTGCTGGTCGAGAACGACGCCGAGGTGCTGGAGGCCATGACCTTCCTGCTCGA
GCGCTGGCAGTGCCTGGTGCGGGCCGCGACCTCGACCGACGACGCGCTGGACCTGCTCGGCGACACCGACTGGGTGCCCG
ACATCATCATTGCCGACCAGCATCTCGACGGCGGCGACCTCGGCACCACCACCATATCGGAAGTCCGCGACTATCTCGGC
CGCGCCGTGCCTGCGCTGATAGTTACCGCCGACAGTTCCGAAGCCGTCGCCAAGGCCGCGCGCGCCGGCGGCATCGAACT
GATGCGCAAACCGCTGAAACCGGCGCAACTAAGGGCGCTGCTGGCGCATTTGCTGGCTTAG

Upstream 100 bases:

>100_bases
GCACCTATGGCGAACAGTATCGGTCGATGCACCTCAACCAGACTTTCACCGGCATCGCCTTCGGCGAACGGCAAGCGGCA
GAATAAGGCGGCGCTATAAG

Downstream 100 bases:

>100_bases
AGTGAGCCCTTCTCCCCGTCTCTATACGGGGAGAAGGTGGCGGCAGCCGGATGAGGGGCGGCGCCGGCGCTTGCGATCTT
TCTTCGGCGGTGGGAAAGAA

Product: two-component sensor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPLRDINDLEKLKKINAALVDRVERSMDQQGNAFSLFQTAISLENRVRTRTEELHSTLRRLEQSNIDLSAAKENAEFANL
SKTRFLAAASHDVLQPLNAAHLSVSALAELQTSDEGRKLVRQVERSLETMEDLLRTLLDISKLDAGVVHPDIGDVSLEAL
FSSLRSDFQPVAEMKRLSLKFRPVNAVVRSDRTLLRRILQNILSNALGYTRSGGVLVGTRHRGDTIRIDVADTGCGIPED
QREAVFEEFHRGTLPANAGLDGGGLGLGLAIVRRMAGALGHPVTFSSKVGRGTIFHIDVPVGIGAPADAIASATSLERSR
GYGLFGTKVLLVENDAEVLEAMTFLLERWQCLVRAATSTDDALDLLGDTDWVPDIIIADQHLDGGDLGTTTISEVRDYLG
RAVPALIVTADSSEAVAKAARAGGIELMRKPLKPAQLRALLAHLLA

Sequences:

>Translated_446_residues
MPLRDINDLEKLKKINAALVDRVERSMDQQGNAFSLFQTAISLENRVRTRTEELHSTLRRLEQSNIDLSAAKENAEFANL
SKTRFLAAASHDVLQPLNAAHLSVSALAELQTSDEGRKLVRQVERSLETMEDLLRTLLDISKLDAGVVHPDIGDVSLEAL
FSSLRSDFQPVAEMKRLSLKFRPVNAVVRSDRTLLRRILQNILSNALGYTRSGGVLVGTRHRGDTIRIDVADTGCGIPED
QREAVFEEFHRGTLPANAGLDGGGLGLGLAIVRRMAGALGHPVTFSSKVGRGTIFHIDVPVGIGAPADAIASATSLERSR
GYGLFGTKVLLVENDAEVLEAMTFLLERWQCLVRAATSTDDALDLLGDTDWVPDIIIADQHLDGGDLGTTTISEVRDYLG
RAVPALIVTADSSEAVAKAARAGGIELMRKPLKPAQLRALLAHLLA
>Mature_445_residues
PLRDINDLEKLKKINAALVDRVERSMDQQGNAFSLFQTAISLENRVRTRTEELHSTLRRLEQSNIDLSAAKENAEFANLS
KTRFLAAASHDVLQPLNAAHLSVSALAELQTSDEGRKLVRQVERSLETMEDLLRTLLDISKLDAGVVHPDIGDVSLEALF
SSLRSDFQPVAEMKRLSLKFRPVNAVVRSDRTLLRRILQNILSNALGYTRSGGVLVGTRHRGDTIRIDVADTGCGIPEDQ
REAVFEEFHRGTLPANAGLDGGGLGLGLAIVRRMAGALGHPVTFSSKVGRGTIFHIDVPVGIGAPADAIASATSLERSRG
YGLFGTKVLLVENDAEVLEAMTFLLERWQCLVRAATSTDDALDLLGDTDWVPDIIIADQHLDGGDLGTTTISEVRDYLGR
AVPALIVTADSSEAVAKAARAGGIELMRKPLKPAQLRALLAHLLA

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=377, Percent_Identity=27.0557029177719, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789149, Length=286, Percent_Identity=29.020979020979, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87081816, Length=442, Percent_Identity=24.6606334841629, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693157, Length=348, Percent_Identity=24.1379310344828, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=27.0161290322581, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082128, Length=251, Percent_Identity=26.6932270916335, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=27.5720164609054, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788393, Length=193, Percent_Identity=30.0518134715026, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788549, Length=284, Percent_Identity=27.112676056338, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=227, Percent_Identity=29.0748898678414, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786783, Length=242, Percent_Identity=26.4462809917355, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48338; Mature: 48207

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRDINDLEKLKKINAALVDRVERSMDQQGNAFSLFQTAISLENRVRTRTEELHSTLRR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQSNIDLSAAKENAEFANLSKTRFLAAASHDVLQPLNAAHLSVSALAELQTSDEGRKLV
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RQVERSLETMEDLLRTLLDISKLDAGVVHPDIGDVSLEALFSSLRSDFQPVAEMKRLSLK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
FRPVNAVVRSDRTLLRRILQNILSNALGYTRSGGVLVGTRHRGDTIRIDVADTGCGIPED
ECHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCH
QREAVFEEFHRGTLPANAGLDGGGLGLGLAIVRRMAGALGHPVTFSSKVGRGTIFHIDVP
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEC
VGIGAPADAIASATSLERSRGYGLFGTKVLLVENDAEVLEAMTFLLERWQCLVRAATSTD
CCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
DALDLLGDTDWVPDIIIADQHLDGGDLGTTTISEVRDYLGRAVPALIVTADSSEAVAKAA
HHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHH
RAGGIELMRKPLKPAQLRALLAHLLA
HHCCHHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
PLRDINDLEKLKKINAALVDRVERSMDQQGNAFSLFQTAISLENRVRTRTEELHSTLRR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQSNIDLSAAKENAEFANLSKTRFLAAASHDVLQPLNAAHLSVSALAELQTSDEGRKLV
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RQVERSLETMEDLLRTLLDISKLDAGVVHPDIGDVSLEALFSSLRSDFQPVAEMKRLSLK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
FRPVNAVVRSDRTLLRRILQNILSNALGYTRSGGVLVGTRHRGDTIRIDVADTGCGIPED
ECHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCH
QREAVFEEFHRGTLPANAGLDGGGLGLGLAIVRRMAGALGHPVTFSSKVGRGTIFHIDVP
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEC
VGIGAPADAIASATSLERSRGYGLFGTKVLLVENDAEVLEAMTFLLERWQCLVRAATSTD
CCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
DALDLLGDTDWVPDIIIADQHLDGGDLGTTTISEVRDYLGRAVPALIVTADSSEAVAKAA
HHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHH
RAGGIELMRKPLKPAQLRALLAHLLA
HHCCHHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]