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The map label for this gene is gatA

Identifier: 13470863

GI number: 13470863

Start: 529288

End: 530853

Strand: Reverse

Name: gatA

Synonym: mll0675

Alternate gene names: 13470863

Gene position: 530853-529288 (Counterclockwise)

Preceding gene: 13470864

Following gene: 13470862

Centisome position: 7.54

GC content: 65.77

Gene sequence:

>1566_bases
ATGAGCGACCTGACCAGACTGACGATTTCACAGGCTCGCGCCAAGCTGCGCGGCAAGGAAATCACCGCGACAGAGATCAC
CGAGGCCTATCTCTCGGCGATCGATCGCGCCAATCCGGCGCTCAATGCCTATGTCGCGGTGACTGGCGACAGAGCGCGTG
ACATGGCCAAGGCGTCCGACGCCAGGCTGGTCAAGGGCGAGGGCGGGGCGCTGGAGGGCATTCCGCTGGGCATCAAGGAC
CTGTTCGGCACCGAAGGTGTCCACACCCAGGCCTGCAGCCACGTGCTCGACGGCTTCAAGCCGCTCTACGAATCGACTGT
CACCGCCAATCTGTGGGCGGATGGCGCGGTCATGCTCGGCAAGCTCAACATGGACGAGTTCGCCATGGGCTCGTCCAACG
AGACCTCCTATTACGGCCCGGTCATCAACCCGTGGCGGCGTTCGCGCCTCGACACGGTGGTGATGCCGACGACGCATCAG
GGCGACGGCGGTTTTGTCTCGGCCGGCGGCACGAAAACCCAGCGCAGCCTGGACAACGCGCAGCTGGTTCCGGGTGGCTC
TTCCGGCGGCTCGGCGACGGCCGTCTCGGCGTTCCTGTGCGCCGGCGCCACGGCGACCGATACGGGCGGCTCGATCCGCC
AGCCGGCGGCCTTCACCGGCACCGTCGGCATCAAGCCGACCTATGGCCGCTGCTCGCGCTGGGGCATCGTCGCCTTCGCC
TCGTCGCTCGACCAGGCCGGACCGATCGCGCGCGATGTTCGCGACGCCGCGATCCTGCTCAAATCCATGGCCTCGGTCGA
TCCGAAGGACACCACTTCCGTCGACCGGCCGGTGCCGGACTATGAGGCGGCGATCGGCAAGCCGATCAAGGGCATGAAGG
TCGGCATTCCCAAGGAATACCGCGTTGACGGCATGCCCGAGGAGATCGAGGCGCTGTGGCAGAAGGGCATTGCCTGGCTC
AGGGATGCCGGCGCCGAGATCGTCGACATTTCGCTGCCGCATACGAAATACGCGCTGCCGGCCTATTACATCGTGGCGCC
CGCCGAGGCTTCGTCCAACCTGGCGCGCTATGACGGCGTCCGCTACGGCTTGCGCGTGCCGGGCAAGGACATTGTCGAGA
TGTATGAGAAGACCCGCGCCGCCGGTTTCGGCCGCGAGGTCAAGCGCCGCATCATGATCGGCACCTATGTGCTGTCGGCC
GGCTATTACGACGCCTATTATCTGCAGGCACAGAAGGTGCGCAATTTGATCAAGCGCGACTTCGAAAATGTCTTCGCCGC
CGGCGTCGACGTCATCCTGACCCCGGCGACGCCGTCCGCCGCCTTCGGCATCGCCGATGAGGACATGGCCGCCGACCCGG
TGAAAATGTACCTCAACGACATTTTCACGGTCACCGTGAACATGGCGGGCCTGCCCGGCATCGCCGTGCCCGCCGGCCTC
GATCCCAAGGGGCTGCCGCTCGGCCTGCAGCTGATCGGCCGGCCGTTCGAGGAAGAGACGCTGTTCCAGACCGCCGCCGT
CATCGAGCAGGCGGCCGGCACATTTCAGCCTGAGAAGTGGTGGTAA

Upstream 100 bases:

>100_bases
CCGCCTGGACCGTCTACGAACGTGCCGGGTTTTCGCGCTGCGGCCCGGTGCTGGATTATCCCGATTCCAAGTGGTCGGTG
TTTTACGAAAAGAGCCTTTG

Downstream 100 bases:

>100_bases
GGATATCGATCATCTGAAGGGGTGAGCGATGTTCTTCCTTCTTTCCAAGGTCTTCTGGTTCTTCGTCCAGCCGCTCAACC
TGACGATCTTCCTGCTTCTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSDLTRLTISQARAKLRGKEITATEITEAYLSAIDRANPALNAYVAVTGDRARDMAKASDARLVKGEGGALEGIPLGIKD
LFGTEGVHTQACSHVLDGFKPLYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVINPWRRSRLDTVVMPTTHQ
GDGGFVSAGGTKTQRSLDNAQLVPGGSSGGSATAVSAFLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFA
SSLDQAGPIARDVRDAAILLKSMASVDPKDTTSVDRPVPDYEAAIGKPIKGMKVGIPKEYRVDGMPEEIEALWQKGIAWL
RDAGAEIVDISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIVEMYEKTRAAGFGREVKRRIMIGTYVLSA
GYYDAYYLQAQKVRNLIKRDFENVFAAGVDVILTPATPSAAFGIADEDMAADPVKMYLNDIFTVTVNMAGLPGIAVPAGL
DPKGLPLGLQLIGRPFEEETLFQTAAVIEQAAGTFQPEKWW

Sequences:

>Translated_521_residues
MSDLTRLTISQARAKLRGKEITATEITEAYLSAIDRANPALNAYVAVTGDRARDMAKASDARLVKGEGGALEGIPLGIKD
LFGTEGVHTQACSHVLDGFKPLYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVINPWRRSRLDTVVMPTTHQ
GDGGFVSAGGTKTQRSLDNAQLVPGGSSGGSATAVSAFLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFA
SSLDQAGPIARDVRDAAILLKSMASVDPKDTTSVDRPVPDYEAAIGKPIKGMKVGIPKEYRVDGMPEEIEALWQKGIAWL
RDAGAEIVDISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIVEMYEKTRAAGFGREVKRRIMIGTYVLSA
GYYDAYYLQAQKVRNLIKRDFENVFAAGVDVILTPATPSAAFGIADEDMAADPVKMYLNDIFTVTVNMAGLPGIAVPAGL
DPKGLPLGLQLIGRPFEEETLFQTAAVIEQAAGTFQPEKWW
>Mature_520_residues
SDLTRLTISQARAKLRGKEITATEITEAYLSAIDRANPALNAYVAVTGDRARDMAKASDARLVKGEGGALEGIPLGIKDL
FGTEGVHTQACSHVLDGFKPLYESTVTANLWADGAVMLGKLNMDEFAMGSSNETSYYGPVINPWRRSRLDTVVMPTTHQG
DGGFVSAGGTKTQRSLDNAQLVPGGSSGGSATAVSAFLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFAS
SLDQAGPIARDVRDAAILLKSMASVDPKDTTSVDRPVPDYEAAIGKPIKGMKVGIPKEYRVDGMPEEIEALWQKGIAWLR
DAGAEIVDISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIVEMYEKTRAAGFGREVKRRIMIGTYVLSAG
YYDAYYLQAQKVRNLIKRDFENVFAAGVDVILTPATPSAAFGIADEDMAADPVKMYLNDIFTVTVNMAGLPGIAVPAGLD
PKGLPLGLQLIGRPFEEETLFQTAAVIEQAAGTFQPEKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=516, Percent_Identity=39.7286821705426, Blast_Score=350, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17543272, Length=478, Percent_Identity=33.2635983263598, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17537465, Length=543, Percent_Identity=23.572744014733, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=460, Percent_Identity=32.8260869565217, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6319685, Length=493, Percent_Identity=25.7606490872211, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648113, Length=511, Percent_Identity=35.812133072407, Blast_Score=276, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24644968, Length=537, Percent_Identity=22.9050279329609, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161078093, Length=551, Percent_Identity=22.8675136116152, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_RHILO (Q98M95)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102432.1
- ProteinModelPortal:   Q98M95
- SMR:   Q98M95
- GeneID:   1225095
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0675
- NMPDR:   fig|266835.1.peg.537
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 55666; Mature: 55535

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 187-187 ACT_SITE 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLTRLTISQARAKLRGKEITATEITEAYLSAIDRANPALNAYVAVTGDRARDMAKASD
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCC
ARLVKGEGGALEGIPLGIKDLFGTEGVHTQACSHVLDGFKPLYESTVTANLWADGAVMLG
CEEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEE
KLNMDEFAMGSSNETSYYGPVINPWRRSRLDTVVMPTTHQGDGGFVSAGGTKTQRSLDNA
ECCHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCEEECCCCHHHHCCCCC
QLVPGGSSGGSATAVSAFLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFA
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHH
SSLDQAGPIARDVRDAAILLKSMASVDPKDTTSVDRPVPDYEAAIGKPIKGMKVGIPKEY
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCEECCCCCC
RVDGMPEEIEALWQKGIAWLRDAGAEIVDISLPHTKYALPAYYIVAPAEASSNLARYDGV
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHCCC
RYGLRVPGKDIVEMYEKTRAAGFGREVKRRIMIGTYVLSAGYYDAYYLQAQKVRNLIKRD
EEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FENVFAAGVDVILTPATPSAAFGIADEDMAADPVKMYLNDIFTVTVNMAGLPGIAVPAGL
HHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCC
DPKGLPLGLQLIGRPFEEETLFQTAAVIEQAAGTFQPEKWW
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDLTRLTISQARAKLRGKEITATEITEAYLSAIDRANPALNAYVAVTGDRARDMAKASD
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCC
ARLVKGEGGALEGIPLGIKDLFGTEGVHTQACSHVLDGFKPLYESTVTANLWADGAVMLG
CEEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEE
KLNMDEFAMGSSNETSYYGPVINPWRRSRLDTVVMPTTHQGDGGFVSAGGTKTQRSLDNA
ECCHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCEEECCCCHHHHCCCCC
QLVPGGSSGGSATAVSAFLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFA
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHH
SSLDQAGPIARDVRDAAILLKSMASVDPKDTTSVDRPVPDYEAAIGKPIKGMKVGIPKEY
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCEECCCCCC
RVDGMPEEIEALWQKGIAWLRDAGAEIVDISLPHTKYALPAYYIVAPAEASSNLARYDGV
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHCCC
RYGLRVPGKDIVEMYEKTRAAGFGREVKRRIMIGTYVLSAGYYDAYYLQAQKVRNLIKRD
EEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FENVFAAGVDVILTPATPSAAFGIADEDMAADPVKMYLNDIFTVTVNMAGLPGIAVPAGL
HHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCC
DPKGLPLGLQLIGRPFEEETLFQTAAVIEQAAGTFQPEKWW
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968