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The map label for this gene is gsiB [H]

Identifier: 13470802

GI number: 13470802

Start: 465243

End: 466904

Strand: Reverse

Name: gsiB [H]

Synonym: mll0599

Alternate gene names: 13470802

Gene position: 466904-465243 (Counterclockwise)

Preceding gene: 13470803

Following gene: 13470801

Centisome position: 6.64

GC content: 61.43

Gene sequence:

>1662_bases
ATGAAGAAACTGCTTTTGGCAGCATCCGCCGCGGCACTGCTGGCCGGCACGTGGCTCGCTCCGGCACAGGCTGAGTATCT
CAAGGAGCACCGAGGCGGCACCATCCGCCTTCTGGCCCGCTCTGCGGCGGGAACGCTCGATCCGCACATCAACTACACCG
ATCAGGGCTGGCAGATGTACCAGCCGATCTATGACGGCCTGGTGGCCTTCCGCAAGGCCGAGGGCATGGACGGCTTCACC
ATCGTCCCCGATCTGGCCGAGGCGCTGCCGCAGGTCAGCAATGACGGCAAGACCTTCACGTTCAAGCTGCGCAAGGGCAT
CAAGTTCTCCAGTGGCCAGGATATGGGCGTGAAGGACGTCGTCGCCTCCTTCCAGCGCATCTTCAAGGTTTCCGGGCCGA
CTTCCGGCACCTTCTATGCCGGCATCGTCGGCGCCGACAAATGCCTGGCCGACACCAAGAACTGCACGCTCGAAGGCGGC
GTTGTCGGCGACGAGGCGGCCGGCACCATCACGCTCAATCTCACCAAGCCCGACGCCGAGATCCTCTACAAGCTTGCCTT
GCCGCACGCCGTGGTACTGCCGGCGGATACGCCCGCCGAAGACATGGGCTCCAAGCCCATTCCCAGCACCGGTGCCTACA
TGATCTCCGCCTTCGACCCCAACAAGGGCATGACGGTGTCGCGCAACCCGAACTTCAAGCAGTGGAGCGAGGAAGCGCAG
CCGGACGGCTATCCGGATGTCGTCCAGTATGATTTCGGCCTGTCCGAGGAAGCGGCCGTGACGGCGATCCAGAACGGCGA
AGCGGACTGGATGTTCGATGCGCTGCCGAGCGACCGCCTGGGCGAACTTGGCAGCAAGTCGATGGACCAGTTGCACATCT
CGCCGCTTTCGGCGTGGTGGTATGCGCCGCTGAACAACCGTCTGGCACCCTTCGACAACGAAAAGGCGCGCCAGGCCGTT
GCCTATGCGATCGATCGCAACACGCTGGTCAAGCTGTTCGGCGGCAAGGTGCTGGCCTCGCCGGTCTGCCAGGTCCTGCC
GCCGGACTTCCCGGGCCATGAGGACTATTGCCCGTTCACCAAAAACCCCGGCGCCAAATGGTCGGCACCCGATCTCGACA
AGGCAAAGCAGCTTGTCGAGGAATCCGGCACCAAGGGCCAGAAGGTGACCATCATCGCCGAGGACACAGCCATCTCACGC
TCCATCGGCGTCTATCTGCAGAGCGTGCTGACCAGCATCGGCTATGTCGCCGACGTCAAGCCGATCTCGCCCAATATCCA
GTTCACCTACATTCAGAACACCAACAACAAAGTGCAGATGTCCATTACCCAGTGGTACAAGGACTATCCCGCAGCGTCGG
ACTTCCTCAACATCCTGTTCAGCTGCTCCTCCTTCCGTGAAGGTTCGGACGCTTCGATCAACATCGCCGGCTTCTGCGAC
AAGGACATCGACGCCAAGATGCAAAAGGCGCTGGACCTCGGCGTGACCGACCAGAAAGCCGCCGACAAAATGTGGGCCGA
AATCGACAAGCAGGTCACGGACAAGGCGCCGGCAGTCGGCCTCTTTACCCCGAAGCGGCTTGATTTCGTCAGCAAACGGG
TTGGCAACTTCAAGTTCAACCGGCAGTTCAACTGGATGATCACCCAGTCCTGGGTGCAGTAA

Upstream 100 bases:

>100_bases
TTCCAGCCACATGCCGCATGTCGAAGAGCAGGACGCGTGCATGCGGGTGGTGGGCGACTTTCTGGACGACAACGACAACT
GAGAAAAGGGGAACCAAGAC

Downstream 100 bases:

>100_bases
CGACCTTTGGAGGGGCACGCCCGTGTCGAACCAAGCCGTCGCCATGTCGCGCAAGACGCGAGGGCCCTGGGCCGTCGCGT
TCGCGAAGCTGGCAAGGGAC

Product: hypothetical protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MKKLLLAASAAALLAGTWLAPAQAEYLKEHRGGTIRLLARSAAGTLDPHINYTDQGWQMYQPIYDGLVAFRKAEGMDGFT
IVPDLAEALPQVSNDGKTFTFKLRKGIKFSSGQDMGVKDVVASFQRIFKVSGPTSGTFYAGIVGADKCLADTKNCTLEGG
VVGDEAAGTITLNLTKPDAEILYKLALPHAVVLPADTPAEDMGSKPIPSTGAYMISAFDPNKGMTVSRNPNFKQWSEEAQ
PDGYPDVVQYDFGLSEEAAVTAIQNGEADWMFDALPSDRLGELGSKSMDQLHISPLSAWWYAPLNNRLAPFDNEKARQAV
AYAIDRNTLVKLFGGKVLASPVCQVLPPDFPGHEDYCPFTKNPGAKWSAPDLDKAKQLVEESGTKGQKVTIIAEDTAISR
SIGVYLQSVLTSIGYVADVKPISPNIQFTYIQNTNNKVQMSITQWYKDYPAASDFLNILFSCSSFREGSDASINIAGFCD
KDIDAKMQKALDLGVTDQKAADKMWAEIDKQVTDKAPAVGLFTPKRLDFVSKRVGNFKFNRQFNWMITQSWVQ

Sequences:

>Translated_553_residues
MKKLLLAASAAALLAGTWLAPAQAEYLKEHRGGTIRLLARSAAGTLDPHINYTDQGWQMYQPIYDGLVAFRKAEGMDGFT
IVPDLAEALPQVSNDGKTFTFKLRKGIKFSSGQDMGVKDVVASFQRIFKVSGPTSGTFYAGIVGADKCLADTKNCTLEGG
VVGDEAAGTITLNLTKPDAEILYKLALPHAVVLPADTPAEDMGSKPIPSTGAYMISAFDPNKGMTVSRNPNFKQWSEEAQ
PDGYPDVVQYDFGLSEEAAVTAIQNGEADWMFDALPSDRLGELGSKSMDQLHISPLSAWWYAPLNNRLAPFDNEKARQAV
AYAIDRNTLVKLFGGKVLASPVCQVLPPDFPGHEDYCPFTKNPGAKWSAPDLDKAKQLVEESGTKGQKVTIIAEDTAISR
SIGVYLQSVLTSIGYVADVKPISPNIQFTYIQNTNNKVQMSITQWYKDYPAASDFLNILFSCSSFREGSDASINIAGFCD
KDIDAKMQKALDLGVTDQKAADKMWAEIDKQVTDKAPAVGLFTPKRLDFVSKRVGNFKFNRQFNWMITQSWVQ
>Mature_553_residues
MKKLLLAASAAALLAGTWLAPAQAEYLKEHRGGTIRLLARSAAGTLDPHINYTDQGWQMYQPIYDGLVAFRKAEGMDGFT
IVPDLAEALPQVSNDGKTFTFKLRKGIKFSSGQDMGVKDVVASFQRIFKVSGPTSGTFYAGIVGADKCLADTKNCTLEGG
VVGDEAAGTITLNLTKPDAEILYKLALPHAVVLPADTPAEDMGSKPIPSTGAYMISAFDPNKGMTVSRNPNFKQWSEEAQ
PDGYPDVVQYDFGLSEEAAVTAIQNGEADWMFDALPSDRLGELGSKSMDQLHISPLSAWWYAPLNNRLAPFDNEKARQAV
AYAIDRNTLVKLFGGKVLASPVCQVLPPDFPGHEDYCPFTKNPGAKWSAPDLDKAKQLVEESGTKGQKVTIIAEDTAISR
SIGVYLQSVLTSIGYVADVKPISPNIQFTYIQNTNNKVQMSITQWYKDYPAASDFLNILFSCSSFREGSDASINIAGFCD
KDIDAKMQKALDLGVTDQKAADKMWAEIDKQVTDKAPAVGLFTPKRLDFVSKRVGNFKFNRQFNWMITQSWVQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=569, Percent_Identity=23.9015817223199, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=24.703557312253, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789966, Length=512, Percent_Identity=25, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1789397, Length=547, Percent_Identity=23.0347349177331, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787762, Length=509, Percent_Identity=24.1650294695481, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787551, Length=433, Percent_Identity=23.5565819861432, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60430; Mature: 60430

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLAASAAALLAGTWLAPAQAEYLKEHRGGTIRLLARSAAGTLDPHINYTDQGWQMY
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHH
QPIYDGLVAFRKAEGMDGFTIVPDLAEALPQVSNDGKTFTFKLRKGIKFSSGQDMGVKDV
HHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCHHHH
VASFQRIFKVSGPTSGTFYAGIVGADKCLADTKNCTLEGGVVGDEAAGTITLNLTKPDAE
HHHHHHHHCCCCCCCCCEEEEEECCHHHHCCCCCCEEECCEECCCCCCEEEEEEECCHHH
ILYKLALPHAVVLPADTPAEDMGSKPIPSTGAYMISAFDPNKGMTVSRNPNFKQWSEEAQ
HHHHHHCCCEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHCC
PDGYPDVVQYDFGLSEEAAVTAIQNGEADWMFDALPSDRLGELGSKSMDQLHISPLSAWW
CCCCCCCEEECCCCCCCHHEEEEECCCCCEEEECCCCHHHHHHCCCCHHHEECCEEEEEE
YAPLNNRLAPFDNEKARQAVAYAIDRNTLVKLFGGKVLASPVCQVLPPDFPGHEDYCPFT
ECCCCCCCCCCCCHHHHHHHHHHHCCCCEEHHHCCHHHHCCHHHHCCCCCCCCCCCCCCC
KNPGAKWSAPDLDKAKQLVEESGTKGQKVTIIAEDTAISRSIGVYLQSVLTSIGYVADVK
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEEECC
PISPNIQFTYIQNTNNKVQMSITQWYKDYPAASDFLNILFSCSSFREGSDASINIAGFCD
CCCCCEEEEEEECCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
KDIDAKMQKALDLGVTDQKAADKMWAEIDKQVTDKAPAVGLFTPKRLDFVSKRVGNFKFN
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCEEEC
RQFNWMITQSWVQ
CEEEEEEEHHHCC
>Mature Secondary Structure
MKKLLLAASAAALLAGTWLAPAQAEYLKEHRGGTIRLLARSAAGTLDPHINYTDQGWQMY
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHH
QPIYDGLVAFRKAEGMDGFTIVPDLAEALPQVSNDGKTFTFKLRKGIKFSSGQDMGVKDV
HHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCHHHH
VASFQRIFKVSGPTSGTFYAGIVGADKCLADTKNCTLEGGVVGDEAAGTITLNLTKPDAE
HHHHHHHHCCCCCCCCCEEEEEECCHHHHCCCCCCEEECCEECCCCCCEEEEEEECCHHH
ILYKLALPHAVVLPADTPAEDMGSKPIPSTGAYMISAFDPNKGMTVSRNPNFKQWSEEAQ
HHHHHHCCCEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHCC
PDGYPDVVQYDFGLSEEAAVTAIQNGEADWMFDALPSDRLGELGSKSMDQLHISPLSAWW
CCCCCCCEEECCCCCCCHHEEEEECCCCCEEEECCCCHHHHHHCCCCHHHEECCEEEEEE
YAPLNNRLAPFDNEKARQAVAYAIDRNTLVKLFGGKVLASPVCQVLPPDFPGHEDYCPFT
ECCCCCCCCCCCCHHHHHHHHHHHCCCCEEHHHCCHHHHCCHHHHCCCCCCCCCCCCCCC
KNPGAKWSAPDLDKAKQLVEESGTKGQKVTIIAEDTAISRSIGVYLQSVLTSIGYVADVK
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEEECC
PISPNIQFTYIQNTNNKVQMSITQWYKDYPAASDFLNILFSCSSFREGSDASINIAGFCD
CCCCCEEEEEEECCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
KDIDAKMQKALDLGVTDQKAADKMWAEIDKQVTDKAPAVGLFTPKRLDFVSKRVGNFKFN
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCEEEC
RQFNWMITQSWVQ
CEEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA