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The map label for this gene is rlmB [H]

Identifier: 13470525

GI number: 13470525

Start: 181281

End: 181988

Strand: Reverse

Name: rlmB [H]

Synonym: mll0255

Alternate gene names: 13470525

Gene position: 181988-181281 (Counterclockwise)

Preceding gene: 13470526

Following gene: 13470521

Centisome position: 2.59

GC content: 64.55

Gene sequence:

>708_bases
GTGCGACTTTATGGACTTCACACGGTGCGGGCGGCACTCGACAATCCGCGCCGCAAGATTCGCAAAATGCTGGTGACCCG
CAACGCGGCCGAGCGCCTCGAGATAGAAGATCTTGCCGCCCTTACCTTCAAGGCGGAACTGGTCGAACCCAGGGATATCG
ACAAGATCACCGGATCGGATGCCGTGCACCAGGGCGTGCTGATCGAGGCGGAGCCGCTGAAGCCCAAGCGCCTCGACGCG
CTTGGCGACACGAGGCTGGTGCTGGTGCTCGACCAGATCACCGATCCGCACAATGTCGGCGCCATCCTGCGCTCGGCGGT
CGCCTTCGGCGCCGGCGCGCTGATTACCACAGCGCGCCATAGCCCTCAGGAATCGGGCGTTCTAGCGAAATCCGCCTCCG
GTGCGCTGGAACATATCGACCAGATCGAGGTGAAGAACCTGGCCGACGCGCTCGGGCAGTTGCACGAAGCCGGCTTCCAG
ACCATCGGGCTTGATTCGGACGGGCCGGCGGAACTCGAGACAAGCTTTGCCGGCGACAGGATCGCTTTGGTACTCGGCGC
CGAAGGCAAGGGCTTGCGCCAGAAGACGCGCGAGACGGTGACGACGCTGGCGCGGCTGGACATGCCCGGCGCCATCCGCT
CGCTCAATGTATCGAACGCCGCGGCGGTGAGCCTTTATGCTGCACGGGCGTTCTTGAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CAAGCTGCGCCGCGCGCACCGCGACGAGAAGAGCGGTGGCGCGCCGGCATTCCGGCCACGCCAGCCCGTGTCGCCCGGCG
AGAACGCCGCCGACGGCCTG

Downstream 100 bases:

>100_bases
GAGCCGGAAGCGCTCTACAAAAAGACGAGAAACGGGTTCCGGCGACAGCGCGGGAACCCGTTCTTGTGCAGGACAAGAGG
TGGCGGCTTGGGAACGTTGC

Product: tRNA/rRNA metyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRLYGLHTVRAALDNPRRKIRKMLVTRNAAERLEIEDLAALTFKAELVEPRDIDKITGSDAVHQGVLIEAEPLKPKRLDA
LGDTRLVLVLDQITDPHNVGAILRSAVAFGAGALITTARHSPQESGVLAKSASGALEHIDQIEVKNLADALGQLHEAGFQ
TIGLDSDGPAELETSFAGDRIALVLGAEGKGLRQKTRETVTTLARLDMPGAIRSLNVSNAAAVSLYAARAFLKRG

Sequences:

>Translated_235_residues
MRLYGLHTVRAALDNPRRKIRKMLVTRNAAERLEIEDLAALTFKAELVEPRDIDKITGSDAVHQGVLIEAEPLKPKRLDA
LGDTRLVLVLDQITDPHNVGAILRSAVAFGAGALITTARHSPQESGVLAKSASGALEHIDQIEVKNLADALGQLHEAGFQ
TIGLDSDGPAELETSFAGDRIALVLGAEGKGLRQKTRETVTTLARLDMPGAIRSLNVSNAAAVSLYAARAFLKRG
>Mature_235_residues
MRLYGLHTVRAALDNPRRKIRKMLVTRNAAERLEIEDLAALTFKAELVEPRDIDKITGSDAVHQGVLIEAEPLKPKRLDA
LGDTRLVLVLDQITDPHNVGAILRSAVAFGAGALITTARHSPQESGVLAKSASGALEHIDQIEVKNLADALGQLHEAGFQ
TIGLDSDGPAELETSFAGDRIALVLGAEGKGLRQKTRETVTTLARLDMPGAIRSLNVSNAAAVSLYAARAFLKRG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=253, Percent_Identity=32.4110671936759, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1790623, Length=234, Percent_Identity=32.4786324786325, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788935, Length=236, Percent_Identity=26.6949152542373, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=245, Percent_Identity=24.8979591836735, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25149; Mature: 25149

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYGLHTVRAALDNPRRKIRKMLVTRNAAERLEIEDLAALTFKAELVEPRDIDKITGSD
CEEECHHHHHHHHCCHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCC
AVHQGVLIEAEPLKPKRLDALGDTRLVLVLDQITDPHNVGAILRSAVAFGAGALITTARH
HHHCCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHEECCCC
SPQESGVLAKSASGALEHIDQIEVKNLADALGQLHEAGFQTIGLDSDGPAELETSFAGDR
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHCCCCCE
IALVLGAEGKGLRQKTRETVTTLARLDMPGAIRSLNVSNAAAVSLYAARAFLKRG
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLYGLHTVRAALDNPRRKIRKMLVTRNAAERLEIEDLAALTFKAELVEPRDIDKITGSD
CEEECHHHHHHHHCCHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCC
AVHQGVLIEAEPLKPKRLDALGDTRLVLVLDQITDPHNVGAILRSAVAFGAGALITTARH
HHHCCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHEECCCC
SPQESGVLAKSASGALEHIDQIEVKNLADALGQLHEAGFQTIGLDSDGPAELETSFAGDR
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHCCCCCE
IALVLGAEGKGLRQKTRETVTTLARLDMPGAIRSLNVSNAAAVSLYAARAFLKRG
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]