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The map label for this gene is iclR [H]

Identifier: 13470521

GI number: 13470521

Start: 176518

End: 177339

Strand: Reverse

Name: iclR [H]

Synonym: mll0250

Alternate gene names: 13470521

Gene position: 177339-176518 (Counterclockwise)

Preceding gene: 13470525

Following gene: 13470520

Centisome position: 2.52

GC content: 67.27

Gene sequence:

>822_bases
ATGGAAACCACCGAAAAACGCCAGCGCGGCCGTCCGCGCGCTTTCAACGGGCCTGCCGAAGCCAGTTCTGTGCAGTCGCT
GGATCGGGCCTTGCGCATCCTGGCCATTGTGGCGGAGGGCAGCGGCCTGTCGCTGAGCGAGATATCGGCGCAGAGCGGCC
TTGCCGCCTCCACCGCCTACCGCATGCTGACGACGCTCGAAAACCACGGCATGGTCGAGTTCGACACATCGGACCAGCTA
TGGTCGATCGGCGTCGAGACCTATCGTATGGGGGCGGCCTTTCTACGCCGCCGCAAGTTGGTCGACCGCGCCCGCATCGT
TATGCAGGAGTTGATGGAAAGAACCGGCGAGACCGCCAATCTCGGCGTCGCCGAGGATGATTGCGTGGTGTTCGTCAGCC
AGGTCGAGACGCACCAGGCGATCCGCGCTTTCTTCCGCCCGGGCACGCGCAGCCCCTTTCATGCCTCCGGCATCGGCAAG
GCGGTGCTGGCGCATCTGGAACCCGAGCGCGTCGGCACCATTCTGCGGAAGGCCGGCCTGCAGCGCTTCACCGACAGGAC
GCTGTCCGAGATCCCTGCCCTCGCCCACGATCTGGCCGTCATCAAGCAACGCGGCTGGTCGGTCGACGATGAAGAACGGC
ACCCCGGCATGCGCTGCGTGGCGGCCGCCATCTTCAACGAGTTCGGCGAGCCGATCGGCGGCGTCTCCGTATCGGGGCCG
ACCGTGCGGGTGACGCCGGAGCGGCTGGCCGAGATCGGGCCGATGGTGCGCAGTGCCGCGGCCGAGGTGACCCGGATGAT
CGGCGGCGCGCAAGTCGGCTGA

Upstream 100 bases:

>100_bases
GCTGATTTTGTTGAATTTTCGGTTTTCCAGGAATTTGCCACGTCTCCACAGTGAAAAACTTTTTCATTGTAAGTTTGGCG
AAATCGGCGAAACTGGCTTC

Downstream 100 bases:

>100_bases
CCAGTCTGCCCCGGGAAGCGCGCTCCTGGTCGCAATATCGAAGAAATCGAGCCGTATGGCCTTGCCGACACAGCAAGCGC
CGGCGGCATGGCACTCTCTT

Product: transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
METTEKRQRGRPRAFNGPAEASSVQSLDRALRILAIVAEGSGLSLSEISAQSGLAASTAYRMLTTLENHGMVEFDTSDQL
WSIGVETYRMGAAFLRRRKLVDRARIVMQELMERTGETANLGVAEDDCVVFVSQVETHQAIRAFFRPGTRSPFHASGIGK
AVLAHLEPERVGTILRKAGLQRFTDRTLSEIPALAHDLAVIKQRGWSVDDEERHPGMRCVAAAIFNEFGEPIGGVSVSGP
TVRVTPERLAEIGPMVRSAAAEVTRMIGGAQVG

Sequences:

>Translated_273_residues
METTEKRQRGRPRAFNGPAEASSVQSLDRALRILAIVAEGSGLSLSEISAQSGLAASTAYRMLTTLENHGMVEFDTSDQL
WSIGVETYRMGAAFLRRRKLVDRARIVMQELMERTGETANLGVAEDDCVVFVSQVETHQAIRAFFRPGTRSPFHASGIGK
AVLAHLEPERVGTILRKAGLQRFTDRTLSEIPALAHDLAVIKQRGWSVDDEERHPGMRCVAAAIFNEFGEPIGGVSVSGP
TVRVTPERLAEIGPMVRSAAAEVTRMIGGAQVG
>Mature_273_residues
METTEKRQRGRPRAFNGPAEASSVQSLDRALRILAIVAEGSGLSLSEISAQSGLAASTAYRMLTTLENHGMVEFDTSDQL
WSIGVETYRMGAAFLRRRKLVDRARIVMQELMERTGETANLGVAEDDCVVFVSQVETHQAIRAFFRPGTRSPFHASGIGK
AVLAHLEPERVGTILRKAGLQRFTDRTLSEIPALAHDLAVIKQRGWSVDDEERHPGMRCVAAAIFNEFGEPIGGVSVSGP
TVRVTPERLAEIGPMVRSAAAEVTRMIGGAQVG

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=265, Percent_Identity=40, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1786715, Length=266, Percent_Identity=34.9624060150376, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788131, Length=249, Percent_Identity=31.7269076305221, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=30.8943089430894, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788581, Length=251, Percent_Identity=28.2868525896414, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790752, Length=248, Percent_Identity=22.9838709677419, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789999, Length=224, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29654; Mature: 29654

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTEKRQRGRPRAFNGPAEASSVQSLDRALRILAIVAEGSGLSLSEISAQSGLAASTAY
CCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCHHHHHH
RMLTTLENHGMVEFDTSDQLWSIGVETYRMGAAFLRRRKLVDRARIVMQELMERTGETAN
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LGVAEDDCVVFVSQVETHQAIRAFFRPGTRSPFHASGIGKAVLAHLEPERVGTILRKAGL
CCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
QRFTDRTLSEIPALAHDLAVIKQRGWSVDDEERHPGMRCVAAAIFNEFGEPIGGVSVSGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TVRVTPERLAEIGPMVRSAAAEVTRMIGGAQVG
EEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
METTEKRQRGRPRAFNGPAEASSVQSLDRALRILAIVAEGSGLSLSEISAQSGLAASTAY
CCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCHHHHHH
RMLTTLENHGMVEFDTSDQLWSIGVETYRMGAAFLRRRKLVDRARIVMQELMERTGETAN
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LGVAEDDCVVFVSQVETHQAIRAFFRPGTRSPFHASGIGKAVLAHLEPERVGTILRKAGL
CCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
QRFTDRTLSEIPALAHDLAVIKQRGWSVDDEERHPGMRCVAAAIFNEFGEPIGGVSVSGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TVRVTPERLAEIGPMVRSAAAEVTRMIGGAQVG
EEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]