Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 13470491

GI number: 13470491

Start: 148273

End: 149535

Strand: Direct

Name: amiC [H]

Synonym: mlr0213

Alternate gene names: 13470491

Gene position: 148273-149535 (Clockwise)

Preceding gene: 13470489

Following gene: 13470492

Centisome position: 2.11

GC content: 62.0

Gene sequence:

>1263_bases
ATGGGACTGGCGGACTTCACAGACAAGGGATGTCGTGTTGGTGGCCGGATTCTTGGTGCTCTATTTCTCCTGCTGCTGGT
GGCGATTTCCCAGGGCTTTTTCTCTATTGCCAATGCCGCCGATGCGCCGCTGGTGGCAAAAGGCTACAAGATGGCGGGCG
ATGCCACCAAGATGCGCATCGTCATGGATTTCGATCGCGAGCCTGACATCAAATGGTTCCTGCTGCGCGGACCCAATCGT
CTGGTCATCGATCTGGCGAATACCAGGCTGGCCATCGACGCCAAGGATTTGAAGCCACGCGGCCTGGTCAAGGGCGTGCG
CCTGGGCGCGCTCGGCGACGGCGTTTCGCGGCTGATCCTGACCGGCAAGGGACCGTTTGCCGTCGACAAGCTGGATGTGC
TCAAGAATGAGGACGGAACCGGCTACCGCATTGCCATCGACATGTCGGCCGCCTCCGAACGGGAGTTCGACGCCGCCCTT
GCCAACCAGGCGCTGACCACCGGCTCGACGGTTTCGACCGACAAGGGTGGGCGGGTTGGCACCGGACCGGTCTCCAATCC
GGGCCACCGCTTCACCGTCGTCATCGATCCCGGCCATGGCGGCGTCGACGGCGGCGCCGAGGGCCTGAACGGCACCATCG
AGAAGAACGTCACGCTGGCCTTCGCCACGGAACTGCGCGACAAGCTCGCAGCCATCGGCAAATACGATGTCTTCATGACC
CGCGACACCGATGAATATCTGCGCCTGGACGACCGTGTGCGCATCGCCCGCCAGCATGAGGCCGACCTGCTGATTTCGAT
CCATGCCGACACGATCAGCGTCAAGGGCATTCGCGGCGCCACCGTCTACACCGTCTCCGAAAAGGCATCGGATCCGCAGG
CGCAGGCGCTCGCCGACCGCGAAAATCTCTCCGATCAGTTCGCTGGTATGGTCATCAAGGACGACAACAAGGAAGTGACC
GATATCCTGATCGACCTGATCCGCCGCGAGACGCACACCTTTTCGATGAGTTTCGCCCACACCTTGGTCGGCCAGCTGTC
GACCAGCGTCGGGCTCATCAACAATCCGCAGCGTTCCGCGGGCTTCAAGGTGCTGAAGGCACCGGACGTACCATCCGTGC
TGGTCGAACTCGGCTATCTCTCGAATGCCAAGGACGAAGCGCAGCTGCTTAACGCCGAATGGCGCGGCAAGGCCGCACAA
AGCATCACCAATGCCGTTGCGCTGTTCGCGTCCGCCAAGGCAGGGGCTAGAACCGGAGGTTGA

Upstream 100 bases:

>100_bases
CTTCTCTGGATACCAATCGTTAATCGAGTTCGCGGCCTAACCGGCACTTCGACGAAACGAGCGGGCGCCTGGCGCCAAAC
CGGGAGCGACTGTAAGAGAG

Downstream 100 bases:

>100_bases
CATGCCTTGCGGTGCCTGCAACCAGAGGCGGCGTTGCTGGATGACAACACCCGGTGCCTTTATTTCCTGCGCGCGGTCAC
GAAATCAGGCCTTGCCGTAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MGLADFTDKGCRVGGRILGALFLLLLVAISQGFFSIANAADAPLVAKGYKMAGDATKMRIVMDFDREPDIKWFLLRGPNR
LVIDLANTRLAIDAKDLKPRGLVKGVRLGALGDGVSRLILTGKGPFAVDKLDVLKNEDGTGYRIAIDMSAASEREFDAAL
ANQALTTGSTVSTDKGGRVGTGPVSNPGHRFTVVIDPGHGGVDGGAEGLNGTIEKNVTLAFATELRDKLAAIGKYDVFMT
RDTDEYLRLDDRVRIARQHEADLLISIHADTISVKGIRGATVYTVSEKASDPQAQALADRENLSDQFAGMVIKDDNKEVT
DILIDLIRRETHTFSMSFAHTLVGQLSTSVGLINNPQRSAGFKVLKAPDVPSVLVELGYLSNAKDEAQLLNAEWRGKAAQ
SITNAVALFASAKAGARTGG

Sequences:

>Translated_420_residues
MGLADFTDKGCRVGGRILGALFLLLLVAISQGFFSIANAADAPLVAKGYKMAGDATKMRIVMDFDREPDIKWFLLRGPNR
LVIDLANTRLAIDAKDLKPRGLVKGVRLGALGDGVSRLILTGKGPFAVDKLDVLKNEDGTGYRIAIDMSAASEREFDAAL
ANQALTTGSTVSTDKGGRVGTGPVSNPGHRFTVVIDPGHGGVDGGAEGLNGTIEKNVTLAFATELRDKLAAIGKYDVFMT
RDTDEYLRLDDRVRIARQHEADLLISIHADTISVKGIRGATVYTVSEKASDPQAQALADRENLSDQFAGMVIKDDNKEVT
DILIDLIRRETHTFSMSFAHTLVGQLSTSVGLINNPQRSAGFKVLKAPDVPSVLVELGYLSNAKDEAQLLNAEWRGKAAQ
SITNAVALFASAKAGARTGG
>Mature_419_residues
GLADFTDKGCRVGGRILGALFLLLLVAISQGFFSIANAADAPLVAKGYKMAGDATKMRIVMDFDREPDIKWFLLRGPNRL
VIDLANTRLAIDAKDLKPRGLVKGVRLGALGDGVSRLILTGKGPFAVDKLDVLKNEDGTGYRIAIDMSAASEREFDAALA
NQALTTGSTVSTDKGGRVGTGPVSNPGHRFTVVIDPGHGGVDGGAEGLNGTIEKNVTLAFATELRDKLAAIGKYDVFMTR
DTDEYLRLDDRVRIARQHEADLLISIHADTISVKGIRGATVYTVSEKASDPQAQALADRENLSDQFAGMVIKDDNKEVTD
ILIDLIRRETHTFSMSFAHTLVGQLSTSVGLINNPQRSAGFKVLKAPDVPSVLVELGYLSNAKDEAQLLNAEWRGKAAQS
ITNAVALFASAKAGARTGG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=414, Percent_Identity=30.6763285024155, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=39.0909090909091, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790611, Length=382, Percent_Identity=28.7958115183246, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 44794; Mature: 44662

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLADFTDKGCRVGGRILGALFLLLLVAISQGFFSIANAADAPLVAKGYKMAGDATKMRI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCEECCCCEEEEE
VMDFDREPDIKWFLLRGPNRLVIDLANTRLAIDAKDLKPRGLVKGVRLGALGDGVSRLIL
EEECCCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCCHHHCCEEECCCCCCCEEEEE
TGKGPFAVDKLDVLKNEDGTGYRIAIDMSAASEREFDAALANQALTTGSTVSTDKGGRVG
ECCCCCEECHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEECCCCCCCCC
TGPVSNPGHRFTVVIDPGHGGVDGGAEGLNGTIEKNVTLAFATELRDKLAAIGKYDVFMT
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCEEEEEHHHHHHHHHHCCCEEEEEE
RDTDEYLRLDDRVRIARQHEADLLISIHADTISVKGIRGATVYTVSEKASDPQAQALADR
CCCHHHHCCCCHHHHHHCCCCEEEEEEECCEEEECCCCCEEEEEECCCCCCCHHHHHHHH
ENLSDQFAGMVIKDDNKEVTDILIDLIRRETHTFSMSFAHTLVGQLSTSVGLINNPQRSA
HCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCCCCCCC
GFKVLKAPDVPSVLVELGYLSNAKDEAQLLNAEWRGKAAQSITNAVALFASAKAGARTGG
CEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GLADFTDKGCRVGGRILGALFLLLLVAISQGFFSIANAADAPLVAKGYKMAGDATKMRI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCEECCCCEEEEE
VMDFDREPDIKWFLLRGPNRLVIDLANTRLAIDAKDLKPRGLVKGVRLGALGDGVSRLIL
EEECCCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCCHHHCCEEECCCCCCCEEEEE
TGKGPFAVDKLDVLKNEDGTGYRIAIDMSAASEREFDAALANQALTTGSTVSTDKGGRVG
ECCCCCEECHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEECCCCCCCCC
TGPVSNPGHRFTVVIDPGHGGVDGGAEGLNGTIEKNVTLAFATELRDKLAAIGKYDVFMT
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCEEEEEHHHHHHHHHHCCCEEEEEE
RDTDEYLRLDDRVRIARQHEADLLISIHADTISVKGIRGATVYTVSEKASDPQAQALADR
CCCHHHHCCCCHHHHHHCCCCEEEEEEECCEEEECCCCCEEEEEECCCCCCCHHHHHHHH
ENLSDQFAGMVIKDDNKEVTDILIDLIRRETHTFSMSFAHTLVGQLSTSVGLINNPQRSA
HCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCCCCCCC
GFKVLKAPDVPSVLVELGYLSNAKDEAQLLNAEWRGKAAQSITNAVALFASAKAGARTGG
CEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]