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The map label for this gene is purB [H]

Identifier: 13470385

GI number: 13470385

Start: 54935

End: 56242

Strand: Reverse

Name: purB [H]

Synonym: mll0079

Alternate gene names: 13470385

Gene position: 56242-54935 (Counterclockwise)

Preceding gene: 13470386

Following gene: 13470383

Centisome position: 0.8

GC content: 63.0

Gene sequence:

>1308_bases
ATGATCCCTCGCTATTCCCGGCCGGAAATGGTCGCCATCTGGTCGCCCGAAACCCGGTTTCGCATCTGGTTCGAAATCGA
GGCCCATGCCTGCGACGCGCTGGCCGAACTCGGCGTCATCCCGAAGGAAGCGGCAAAGACCATCTGGGAAAAGGGCGGGG
CCGCGAAATTCGACGTCGAGAAGATCGACGAGATCGAACGCGTCACCAAGCATGACGTCATCGCGTTCCTCACACATCTT
GCCGAATTCGTCGGACCGGATGCGCGTTTCATCCACCAGGGCATGACGTCTTCGGATGTGCTGGACACCTGCTTTGCCGT
CCAGCTTACCCGCGCCAGCGACATTCTGCTCGCAGACATTGACGGCCTGCTTGCCGCGCTCAAGCGCCGCGCCTTCGAAC
ACAAGGACACCGTTACCATCGGCCGCAGCCACGGCATCCATGCCGAGCCGACAACCTTCGGCATCAAGCTGGCGCAGGCC
TATGCCGAATTCTCGCGCTGCCGCGAGCGGCTGGTGCACGCACGCGAAGACATCGCCACCTGCGCCATATCAGGGGCGGT
CGGCACCTTCGCCAACATCGAACCCTATGTCGAGGAGCATGTCGCGGCAAAGCTCGGGCTGAAGCCGGAGCCGGTGTCGA
CGCAAGTGATCCCGCGCGACCGCCACGCCATGTTCTTTGCCACGCTCGGCGTCATCGCCTCGTCGATGGAGCGGCTGGCG
ACCGAGATCCGGCACCTGCAGCGCACCGAGGTGCTGGAAGCGGAAGAGTATTTTTCGCCGGGCCAGAAAGGCTCGTCGGC
AATGCCGCACAAGCGCAATCCGGTGCTGACGGAAAACCTCACCGGCCTTGCCCGCATGGTGCGCTCCATGGCGCTGCCGG
CGATGGAGGATGTGGCGCTCTGGCACGAGCGCGACATCTCGCACTCCTCCGTCGAGCGCATGATTGGTCCGGACGCCACG
GTGACGCTGGATTTCGCGCTGTCACGCCTGACCGGCGTCGTCGACAAGCTGGTCGTCTATCCCGAGAACATGCTGAGGAA
CATGAACAAGTTCCGCGGCCTGGTGCATTCGCAGCGCGTCATGCTGGCGCTGACGCAGGCCGGCCTGTCGCGCGAGGACT
CCTACCGGCTGGTGCAGCGCAACGCCATGAAGGTGTGGGAACAAGGCGCTGATTTTCTTGAGGAACTTTTGGCGGACAAG
GAAGTCACCGCCGCCTTGCCGGAGGCGGAAATCCGTGAGAAATTCGACCTGGGCTACCACACCAAGCATGTCGACACGAT
CTTCAGGCGGGTGTTCGGGGAGGCTTGA

Upstream 100 bases:

>100_bases
GAACCGCCGACCCGCTCTAACTCCTTCTTTTTCGCAATTCCGGACGGAAAACTGCTGCACACTTTTCCTGGAATTGTTCT
AGAGACATCAGGACTGACCG

Downstream 100 bases:

>100_bases
GAACCTAAACGCGCCAAAGAAAAAGGCCCGCCTTTCCGGCGGGCCTTTTCGTATCCGAACTCGCGAAGGACAAAGCCGAT
CACGCCTTTCCCGGAACGGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYSRPEMVAIWSPETRFRIWFEIEAHACDALAELGVIPKEAAKTIWEKGGAAKFDVEKIDEIERVTKHDVIAFLTHL
AEFVGPDARFIHQGMTSSDVLDTCFAVQLTRASDILLADIDGLLAALKRRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQA
YAEFSRCRERLVHAREDIATCAISGAVGTFANIEPYVEEHVAAKLGLKPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSMALPAMEDVALWHERDISHSSVERMIGPDAT
VTLDFALSRLTGVVDKLVVYPENMLRNMNKFRGLVHSQRVMLALTQAGLSREDSYRLVQRNAMKVWEQGADFLEELLADK
EVTAALPEAEIREKFDLGYHTKHVDTIFRRVFGEA

Sequences:

>Translated_435_residues
MIPRYSRPEMVAIWSPETRFRIWFEIEAHACDALAELGVIPKEAAKTIWEKGGAAKFDVEKIDEIERVTKHDVIAFLTHL
AEFVGPDARFIHQGMTSSDVLDTCFAVQLTRASDILLADIDGLLAALKRRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQA
YAEFSRCRERLVHAREDIATCAISGAVGTFANIEPYVEEHVAAKLGLKPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSMALPAMEDVALWHERDISHSSVERMIGPDAT
VTLDFALSRLTGVVDKLVVYPENMLRNMNKFRGLVHSQRVMLALTQAGLSREDSYRLVQRNAMKVWEQGADFLEELLADK
EVTAALPEAEIREKFDLGYHTKHVDTIFRRVFGEA
>Mature_435_residues
MIPRYSRPEMVAIWSPETRFRIWFEIEAHACDALAELGVIPKEAAKTIWEKGGAAKFDVEKIDEIERVTKHDVIAFLTHL
AEFVGPDARFIHQGMTSSDVLDTCFAVQLTRASDILLADIDGLLAALKRRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQA
YAEFSRCRERLVHAREDIATCAISGAVGTFANIEPYVEEHVAAKLGLKPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSMALPAMEDVALWHERDISHSSVERMIGPDAT
VTLDFALSRLTGVVDKLVVYPENMLRNMNKFRGLVHSQRVMLALTQAGLSREDSYRLVQRNAMKVWEQGADFLEELLADK
EVTAALPEAEIREKFDLGYHTKHVDTIFRRVFGEA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=404, Percent_Identity=27.7227722772277, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=27.5590551181102, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1787376, Length=348, Percent_Identity=28.735632183908, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=399, Percent_Identity=26.5664160401003, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=364, Percent_Identity=28.021978021978, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=417, Percent_Identity=28.7769784172662, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24647570, Length=407, Percent_Identity=26.7813267813268, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24640177, Length=214, Percent_Identity=30.8411214953271, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640179, Length=214, Percent_Identity=30.8411214953271, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48829; Mature: 48829

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMVAIWSPETRFRIWFEIEAHACDALAELGVIPKEAAKTIWEKGGAAKFDVE
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
KIDEIERVTKHDVIAFLTHLAEFVGPDARFIHQGMTSSDVLDTCFAVQLTRASDILLADI
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHH
DGLLAALKRRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQAYAEFSRCRERLVHAREDIAT
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIEPYVEEHVAAKLGLKPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
HHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSMALPAMEDVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALSRLTGVVDKLVVYPENMLRNMNKFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MLALTQAGLSREDSYRLVQRNAMKVWEQGADFLEELLADKEVTAALPEAEIREKFDLGYH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCC
TKHVDTIFRRVFGEA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPEMVAIWSPETRFRIWFEIEAHACDALAELGVIPKEAAKTIWEKGGAAKFDVE
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
KIDEIERVTKHDVIAFLTHLAEFVGPDARFIHQGMTSSDVLDTCFAVQLTRASDILLADI
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHH
DGLLAALKRRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQAYAEFSRCRERLVHAREDIAT
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIEPYVEEHVAAKLGLKPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
HHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSMALPAMEDVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALSRLTGVVDKLVVYPENMLRNMNKFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MLALTQAGLSREDSYRLVQRNAMKVWEQGADFLEELLADKEVTAALPEAEIREKFDLGYH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCC
TKHVDTIFRRVFGEA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]