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The map label for this gene is nifS1 [H]

Identifier: 13470342

GI number: 13470342

Start: 12964

End: 14127

Strand: Direct

Name: nifS1 [H]

Synonym: mlr0015

Alternate gene names: 13470342

Gene position: 12964-14127 (Clockwise)

Preceding gene: 13470339

Following gene: 57165208

Centisome position: 0.18

GC content: 66.15

Gene sequence:

>1164_bases
ATGGCCGCAAAACGCGCCTATCTCGACTATAATGCCAGTGCGCCGCTGCTCCCAGCGGCGCGCGAGGCTATGGTCGTGGC
GCTTGACGTGGCCGCCAACCCGTCATCGGTCCATGCCGAGGGCCGCGCCGCGCGCCGCCTGATCGAGACGGCAAGGCGCG
AGGTCGCGGCTCTGGTCAGCGCCAGGCCCGAACATGTCGTTTTCACCTCTGGCGCGACGGAAGCTGCCTCGACGTTGCTC
TCCCCCGAATGGCAGATGGGGCGCGGCAGCGTGCGCATGAGCCGGCTCTACGTCTGCGAGGCCGATCATCCCTGCCTGTT
GAATGGCGGGCGCTTTCCGGCGGCCCAAGTGACGCGCATCGGCGTCGATGCCAACGGCATTGCCGACCTTGCCGCCTTGG
CCGCGGCACTTGGCGATCACGACAAGGCCGATGGCCTACCGCTGGTCGCGATCCACGCCGCCAACAACGAGACCGGCGTC
ATCCAGCCGATCGATCGCATCGCCGAAATCGTCAAGGCCGCTGGCGGCATTCTTGTCGTCGATGCCGTTCAGGCCGCAGG
CCGCGTTTCCATCGACATGTCAGCGGGTTACGCCGATTATTTGATTCAGTCTTCGCACAAGATCGGCGGTCCCAAGGGCG
TCGGCGCCATCGTCGCCGCCGCCGATCTGATGATGCCGAAGCCGCTTATCAATGGCGGCGGCCAGGAAAAGGGCCATCGC
GGTGGCACCGAAAACCTCGCCGCCATATCAGGCTTTGGTGCCGCCGCGCGCGAAGCGCTGGCCGGACTGCTCGCGATTGA
TGCGGTGCGCCAGCGCCGCGACGAGATCGAAGCCATCGTGAAAATGCTGGTGCCGGACGCGGAAATCTTTGGAACCGGTG
CGCCAAGGCTTGCCAACACGACATTCTTCGCTATTGCGGGCATCAAGGCCGAAACCGCGCAGATCGCCTTCGATCTCGCC
GGTGTGGCGCTGTCGGCCGGCTCCGCCTGTTCGTCGGGCAAGGTCGGGCCGAGCCACGTGCTGAAAGCCATGGGCTATGG
CGACAGTCTTGGCGCCTTGCGTGTGTCGATCGGCTCGGCGACGGGCGCCGAGGACATTGAATTGTTCCGCACCGCGCTGG
CGGGAATTGCCCAGCGCCGGGCGGGCAGGGAACAGGCCGCGTGA

Upstream 100 bases:

>100_bases
GCGCCGCAAGCCGCGTAAATAGGACGGCTCGCCTTGGAATTTCAAGGAAATAACGCCATACGACGACCGAATGGCTTCTG
ACGGGATCAACTGGACGAAA

Downstream 100 bases:

>100_bases
CGCGGTGAAAAGGAATTCAGGCTTCGCAGCCTGGTAGAGCCGTGCGTTTGCAAGTGGCCGGGTGAATTTCCGGTCGAAAC
GCACTATATGGAACTTACGC

Product: nitrogenase cofactor synthesis protein nifS

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAAKRAYLDYNASAPLLPAAREAMVVALDVAANPSSVHAEGRAARRLIETARREVAALVSARPEHVVFTSGATEAASTLL
SPEWQMGRGSVRMSRLYVCEADHPCLLNGGRFPAAQVTRIGVDANGIADLAALAAALGDHDKADGLPLVAIHAANNETGV
IQPIDRIAEIVKAAGGILVVDAVQAAGRVSIDMSAGYADYLIQSSHKIGGPKGVGAIVAAADLMMPKPLINGGGQEKGHR
GGTENLAAISGFGAAAREALAGLLAIDAVRQRRDEIEAIVKMLVPDAEIFGTGAPRLANTTFFAIAGIKAETAQIAFDLA
GVALSAGSACSSGKVGPSHVLKAMGYGDSLGALRVSIGSATGAEDIELFRTALAGIAQRRAGREQAA

Sequences:

>Translated_387_residues
MAAKRAYLDYNASAPLLPAAREAMVVALDVAANPSSVHAEGRAARRLIETARREVAALVSARPEHVVFTSGATEAASTLL
SPEWQMGRGSVRMSRLYVCEADHPCLLNGGRFPAAQVTRIGVDANGIADLAALAAALGDHDKADGLPLVAIHAANNETGV
IQPIDRIAEIVKAAGGILVVDAVQAAGRVSIDMSAGYADYLIQSSHKIGGPKGVGAIVAAADLMMPKPLINGGGQEKGHR
GGTENLAAISGFGAAAREALAGLLAIDAVRQRRDEIEAIVKMLVPDAEIFGTGAPRLANTTFFAIAGIKAETAQIAFDLA
GVALSAGSACSSGKVGPSHVLKAMGYGDSLGALRVSIGSATGAEDIELFRTALAGIAQRRAGREQAA
>Mature_386_residues
AAKRAYLDYNASAPLLPAAREAMVVALDVAANPSSVHAEGRAARRLIETARREVAALVSARPEHVVFTSGATEAASTLLS
PEWQMGRGSVRMSRLYVCEADHPCLLNGGRFPAAQVTRIGVDANGIADLAALAAALGDHDKADGLPLVAIHAANNETGVI
QPIDRIAEIVKAAGGILVVDAVQAAGRVSIDMSAGYADYLIQSSHKIGGPKGVGAIVAAADLMMPKPLINGGGQEKGHRG
GTENLAAISGFGAAAREALAGLLAIDAVRQRRDEIEAIVKMLVPDAEIFGTGAPRLANTTFFAIAGIKAETAQIAFDLAG
VALSAGSACSSGKVGPSHVLKAMGYGDSLGALRVSIGSATGAEDIELFRTALAGIAQRRAGREQAA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=373, Percent_Identity=34.5844504021448, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=29.4403892944039, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI48994898, Length=389, Percent_Identity=33.9331619537275, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787970, Length=398, Percent_Identity=24.1206030150754, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=374, Percent_Identity=32.3529411764706, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17533177, Length=314, Percent_Identity=29.9363057324841, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319831, Length=378, Percent_Identity=33.5978835978836, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI20129463, Length=379, Percent_Identity=33.7730870712401, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 39534; Mature: 39402

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKRAYLDYNASAPLLPAAREAMVVALDVAANPSSVHAEGRAARRLIETARREVAALVS
CCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARPEHVVFTSGATEAASTLLSPEWQMGRGSVRMSRLYVCEADHPCLLNGGRFPAAQVTRI
CCCCEEEEECCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCEEEECCCCCHHHEEEE
GVDANGIADLAALAAALGDHDKADGLPLVAIHAANNETGVIQPIDRIAEIVKAAGGILVV
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEE
DAVQAAGRVSIDMSAGYADYLIQSSHKIGGPKGVGAIVAAADLMMPKPLINGGGQEKGHR
EEHHHCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCC
GGTENLAAISGFGAAAREALAGLLAIDAVRQRRDEIEAIVKMLVPDAEIFGTGAPRLANT
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHCC
TFFAIAGIKAETAQIAFDLAGVALSAGSACSSGKVGPSHVLKAMGYGDSLGALRVSIGSA
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCC
TGAEDIELFRTALAGIAQRRAGREQAA
CCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AAKRAYLDYNASAPLLPAAREAMVVALDVAANPSSVHAEGRAARRLIETARREVAALVS
CCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARPEHVVFTSGATEAASTLLSPEWQMGRGSVRMSRLYVCEADHPCLLNGGRFPAAQVTRI
CCCCEEEEECCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCEEEECCCCCHHHEEEE
GVDANGIADLAALAAALGDHDKADGLPLVAIHAANNETGVIQPIDRIAEIVKAAGGILVV
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEE
DAVQAAGRVSIDMSAGYADYLIQSSHKIGGPKGVGAIVAAADLMMPKPLINGGGQEKGHR
EEHHHCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCC
GGTENLAAISGFGAAAREALAGLLAIDAVRQRRDEIEAIVKMLVPDAEIFGTGAPRLANT
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHCC
TFFAIAGIKAETAQIAFDLAGVALSAGSACSSGKVGPSHVLKAMGYGDSLGALRVSIGSA
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCC
TGAEDIELFRTALAGIAQRRAGREQAA
CCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]