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The map label for this gene is iciA [H]

Identifier: 134302789

GI number: 134302789

Start: 1882186

End: 1883106

Strand: Direct

Name: iciA [H]

Synonym: FTW_2010

Alternate gene names: 134302789

Gene position: 1882186-1883106 (Clockwise)

Preceding gene: 134302787

Following gene: 134302790

Centisome position: 99.14

GC content: 28.56

Gene sequence:

>921_bases
ATGATAGATTATAAAGCTATACAGGCATTAAAAGAAGTAATAGAACAACAAAGTTTTGAGCTTGCTGCTGATAAATTATT
TATTAGCCAATCAGCTGTATCACAACGAATAAAATCCCTCGAAACAAAATTTGGGGCACCTGTATTGATAAGGATACTAC
CTTATAAAGTTACAAAATTAGGTCAAGATCTATTAAGCCTCTTAACCAAAGTAGAGTTATTAGAATCTAGCTTACTCGAA
GAAAAAAAGCTTATACAAAATACTAAATTACAAATATCTATAGCTATAAACCAAGATAGTTTGGACACTTGGTTTTTAGA
TGTTTTAAAAGTAATAGATACAACTAACTTAGTAAGGTTTAATTTTAAAACATTTGATCAAGAAATGACTCTTAAATCAT
TACAAAAAGGAGAAGTTTTATCTTGTATCAGTACGATGAAAAAAACTGCTCATGGCTGTTATGTTGAAAAACTTGGTGAA
ATAGAGTATTTATTAGTTTGCTCGCCTTATTTTCATGATAAATATTTCACGAAATTTAAAAGGCGTAATAAAGACTGGGT
AAATACTCTTAAACAAGCACCATCTCTATTATTCAATCAATATGATCAACTAAACTCGAGATTTATGGAGAAATTTTATG
GTTTTCAAGTAGATATAGATAACTGTCATATTGTTCCATCAGTAAAAGGTTTTAAACAGATGTGCTTACAAGGTAAAGGC
TATGCTTTATTACCAAAGTCAGACATTATAGACGAAATAAAAAATGGCGAACTAATTATTTTAGATGATAAATGTATATG
GAATATGGAACTATTTTGGCACTACTGGGACTTGCCAGATAATAACTATCGTAAAATTATGACTACGTTGATTTCAGAAA
GTAAACAAAAGCTTTTAGATATAAAAAATTGTTTATATTAA

Upstream 100 bases:

>100_bases
TTCTCTTATTTTAAAATATTTGATGAAATAAAAATTTAGCAAACAATCAATAATTAGTATAATTAATAATTATAATAATA
CATTAGTTAAATTAATAATT

Downstream 100 bases:

>100_bases
CTCTCAAACCTAACATAGGTTATAAGTATTAATACTATTAGATTATTTGTATCTTTTGGCGCTTTAGCAAACTATTTCCA
AATAAAAATTCTCCAAAATG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIDYKAIQALKEVIEQQSFELAADKLFISQSAVSQRIKSLETKFGAPVLIRILPYKVTKLGQDLLSLLTKVELLESSLLE
EKKLIQNTKLQISIAINQDSLDTWFLDVLKVIDTTNLVRFNFKTFDQEMTLKSLQKGEVLSCISTMKKTAHGCYVEKLGE
IEYLLVCSPYFHDKYFTKFKRRNKDWVNTLKQAPSLLFNQYDQLNSRFMEKFYGFQVDIDNCHIVPSVKGFKQMCLQGKG
YALLPKSDIIDEIKNGELIILDDKCIWNMELFWHYWDLPDNNYRKIMTTLISESKQKLLDIKNCLY

Sequences:

>Translated_306_residues
MIDYKAIQALKEVIEQQSFELAADKLFISQSAVSQRIKSLETKFGAPVLIRILPYKVTKLGQDLLSLLTKVELLESSLLE
EKKLIQNTKLQISIAINQDSLDTWFLDVLKVIDTTNLVRFNFKTFDQEMTLKSLQKGEVLSCISTMKKTAHGCYVEKLGE
IEYLLVCSPYFHDKYFTKFKRRNKDWVNTLKQAPSLLFNQYDQLNSRFMEKFYGFQVDIDNCHIVPSVKGFKQMCLQGKG
YALLPKSDIIDEIKNGELIILDDKCIWNMELFWHYWDLPDNNYRKIMTTLISESKQKLLDIKNCLY
>Mature_306_residues
MIDYKAIQALKEVIEQQSFELAADKLFISQSAVSQRIKSLETKFGAPVLIRILPYKVTKLGQDLLSLLTKVELLESSLLE
EKKLIQNTKLQISIAINQDSLDTWFLDVLKVIDTTNLVRFNFKTFDQEMTLKSLQKGEVLSCISTMKKTAHGCYVEKLGE
IEYLLVCSPYFHDKYFTKFKRRNKDWVNTLKQAPSLLFNQYDQLNSRFMEKFYGFQVDIDNCHIVPSVKGFKQMCLQGKG
YALLPKSDIIDEIKNGELIILDDKCIWNMELFWHYWDLPDNNYRKIMTTLISESKQKLLDIKNCLY

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=289, Percent_Identity=37.3702422145329, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35720; Mature: 35720

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYKAIQALKEVIEQQSFELAADKLFISQSAVSQRIKSLETKFGAPVLIRILPYKVTKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
GQDLLSLLTKVELLESSLLEEKKLIQNTKLQISIAINQDSLDTWFLDVLKVIDTTNLVRF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEE
NFKTFDQEMTLKSLQKGEVLSCISTMKKTAHGCYVEKLGEIEYLLVCSPYFHDKYFTKFK
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCEEEEEEECCHHHHHHHHHHH
RRNKDWVNTLKQAPSLLFNQYDQLNSRFMEKFYGFQVDIDNCHIVPSVKGFKQMCLQGKG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCHHHHHHHHHCCCC
YALLPKSDIIDEIKNGELIILDDKCIWNMELFWHYWDLPDNNYRKIMTTLISESKQKLLD
EEECCCHHHHHHHCCCCEEEEECCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
IKNCLY
HHHHCC
>Mature Secondary Structure
MIDYKAIQALKEVIEQQSFELAADKLFISQSAVSQRIKSLETKFGAPVLIRILPYKVTKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
GQDLLSLLTKVELLESSLLEEKKLIQNTKLQISIAINQDSLDTWFLDVLKVIDTTNLVRF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEE
NFKTFDQEMTLKSLQKGEVLSCISTMKKTAHGCYVEKLGEIEYLLVCSPYFHDKYFTKFK
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCEEEEEEECCHHHHHHHHHHH
RRNKDWVNTLKQAPSLLFNQYDQLNSRFMEKFYGFQVDIDNCHIVPSVKGFKQMCLQGKG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCHHHHHHHHHCCCC
YALLPKSDIIDEIKNGELIILDDKCIWNMELFWHYWDLPDNNYRKIMTTLISESKQKLLD
EEECCCHHHHHHHCCCCEEEEECCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
IKNCLY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA