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The map label for this gene is proP [H]

Identifier: 134302785

GI number: 134302785

Start: 1878691

End: 1879923

Strand: Direct

Name: proP [H]

Synonym: FTW_2006

Alternate gene names: 134302785

Gene position: 1878691-1879923 (Clockwise)

Preceding gene: 134302782

Following gene: 134302787

Centisome position: 98.96

GC content: 30.09

Gene sequence:

>1233_bases
ATGAAAGACTTACAATTAAAAACTATTTTTCTTGGTATGGTTCTAGAATATTTTGACTTTATAATATATCTGAGCTTTTC
TGAAGTAATAGGAACTCATTTACTTCATAGTTCTAACTCCCAACTTATTGTACTTTTGATATTTGCATTAGGTTTTATTA
CAAGACCTATTGGCAGTATTATCTTTGGTCAGTTTGGTGATAGAGTTGGACGCAAAAAAGTATTAATAGCTGCTGTACTT
ATGATGACAATTGGTACGTTAGGTATTGGTCTTATTCCTGATGCTTCCGTTATTGGGATATATGCTGGTTTACTATTACT
TACTTTTCGTATTATGCAAGGTCTTTCTGCTGGTGGAGAGGTCAATAGTGCATTTACATATTTAAATGAAACTATCATTA
CTAACAAAATATATCCTATCGCAGCAATAATGATCTCGGCAGCTATAGGTGTAATGCTTGCTAAACTACTAACAATAATA
CTTAATTTTATTTTCCCTGAGAATTATGCTTGGAGAATTGCTTTTATACTCGGATCTACGCTAGGGATTATTACTTTTTA
TATAAGAAAAGAGCTACATGAGAGTCCTCTTTTTACACAATTATTACAAACTAATCAACTGACAAAAAATCCAATTGTTG
AGCTATTTAAACATTACAAAATGCCGTTATTAGGCTGTTTTTTAATGACTGCTAATGTTATGTCTTGTATTACATTGTTC
TATTATGCTTTTCCGAGCTATTTAGCAAATCTATCTAGTTATAGCTCTAACAGTATGCTCAAGATTAGTATTATAGGCTT
ACTTATGATGTGTATATTTGGCTTACTATCAGCATTACTAATAAAGAAAGATAGCATTCCAAAATTGGATAATTGGTTCT
TACTTTTATCATCAATATTATCTATCTGGTTATGCTATGCTTTTATTCATAACTCACACTATATGGTTGCGTATTTAGTC
TATAGTATTTTATTTAGTGCTTATATCATGATTGCTCAATATAGATGTATTAATGCTCTGCCTGTCAAAGTAAGATTAGT
TGGTATGGGAATTGGTTATGGTTTAGGTTCTGCTGTTTTCGTTGGATTTATGCCTCTTATAACACAGTCTCTTATTTTAG
AGTATCATTCACTCTATATCCATGCTTTTATTGCTATTATTAAAGCAATATTATCTTTAGTAGGTTCTATTATTCTAAAT
ATAACTTCCCATACTAAAAAAATAAATTTATGA

Upstream 100 bases:

>100_bases
ATAGCTTCATTATTTTCATGATATTATCTTACAAATTATCTAACCTAATTATTATGTGATTAGGTACTTTTATTCAAACT
TTGAAATTAGAGATAAAAAA

Downstream 100 bases:

>100_bases
AAAAAAGTATCAGCAGCAAAAGTACAGTAATGAATGTTATGCAAAAGCTTTTTAATGTAAATGTAGGTCTTTTACTTGAA
ATATAACTAGGACAGTGTAT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKDLQLKTIFLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQLIVLLIFALGFITRPIGSIIFGQFGDRVGRKKVLIAAVL
MMTIGTLGIGLIPDASVIGIYAGLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAAIMISAAIGVMLAKLLTII
LNFIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMPLLGCFLMTANVMSCITLF
YYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIKKDSIPKLDNWFLLLSSILSIWLCYAFIHNSHYMVAYLV
YSILFSAYIMIAQYRCINALPVKVRLVGMGIGYGLGSAVFVGFMPLITQSLILEYHSLYIHAFIAIIKAILSLVGSIILN
ITSHTKKINL

Sequences:

>Translated_410_residues
MKDLQLKTIFLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQLIVLLIFALGFITRPIGSIIFGQFGDRVGRKKVLIAAVL
MMTIGTLGIGLIPDASVIGIYAGLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAAIMISAAIGVMLAKLLTII
LNFIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMPLLGCFLMTANVMSCITLF
YYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIKKDSIPKLDNWFLLLSSILSIWLCYAFIHNSHYMVAYLV
YSILFSAYIMIAQYRCINALPVKVRLVGMGIGYGLGSAVFVGFMPLITQSLILEYHSLYIHAFIAIIKAILSLVGSIILN
ITSHTKKINL
>Mature_410_residues
MKDLQLKTIFLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQLIVLLIFALGFITRPIGSIIFGQFGDRVGRKKVLIAAVL
MMTIGTLGIGLIPDASVIGIYAGLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAAIMISAAIGVMLAKLLTII
LNFIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMPLLGCFLMTANVMSCITLF
YYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIKKDSIPKLDNWFLLLSSILSIWLCYAFIHNSHYMVAYLV
YSILFSAYIMIAQYRCINALPVKVRLVGMGIGYGLGSAVFVGFMPLITQSLILEYHSLYIHAFIAIIKAILSLVGSIILN
ITSHTKKINL

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=432, Percent_Identity=28.0092592592593, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1789941, Length=297, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788292, Length=398, Percent_Identity=27.1356783919598, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788942, Length=409, Percent_Identity=27.8728606356968, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082404, Length=399, Percent_Identity=25.062656641604, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082231, Length=268, Percent_Identity=22.7611940298507, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45682; Mature: 45682

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]