Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 134302730

GI number: 134302730

Start: 1812657

End: 1813808

Strand: Direct

Name: bcr [H]

Synonym: FTW_1932

Alternate gene names: 134302730

Gene position: 1812657-1813808 (Clockwise)

Preceding gene: 134302727

Following gene: 134302731

Centisome position: 95.48

GC content: 30.38

Gene sequence:

>1152_bases
ATGAAAACTTACAAAATTATAATTTTACTAACTTACATAAGTATAGCTAGTGCTTCTGCTGTAATTATAAACCCAGCTTT
ACCTAATATTGCTACAGAGCTTGGTTTGTCGAGTGGTACGGTTGAATGGTTGGTAAGTATATTCTTATTAGGCTATGTGC
TAGGTCAGATAATATATGGACCAATTGCAAAGAAATATGGAGATGTCATAACCCTAAGGGCAGGGATGACTATAAATGTT
ATTGGTATACTAGTTTGTATACTAGCTTCTTCTATGACAATGTTGCTTATAGGAAGATTTATAACGGCTATAGGAGCATC
GGCAGGGTTGGTGTGTACTTTTATTATATTAAATAATTCGGTTGCTGCTGAGAAAGCTAAATTAGCTTTATCTTTTGCGA
CTTTATCTTTTGCTATATCGCTAACGCTAGCTACCTTAATAGGTGGAATAATTAGTGCTTATACTCATTGGTATTATTGT
TTTTATGTACTATTGATACATGCTGTAATAATGTTAGGTGCTAGTTTCCTATATGAAAATAAAAAAGACTTTGAATTTAA
TTTAAAGGTATCAGCTATTATTGATGGTTATAAAAATGCTTTTAGTAGTTTTAAATTAGTAGTATTTGCATTGACGCTAG
GTGTTATGACTGTTTTTAGTTATTGTTATTCTGCAGCTGGACCTTTTATAGCTCATAGTATGTTTAATCTAAACAGTACT
CAGTATAGTTTGTGGATTTCGATGACTATTATAGGCATTGTATTAGGTTCAATGTTAGTTGCTAAAATAATAAATAAACA
TGACACTCATAAGATACTTATTGTGGCATTAATTTGTTTTGTTGTGTTAGTTGTAGTTATGGCATGGTTAAAAATAGCTA
ATTATATGACACCGTTAATATTTTTTGTATTAATAACTTTGATGTATTTTGTATGTAATTTTATATACCCTACAGCATCA
TACCTTGCATCAACTGCAATTGAATGTAAGTCAAACGCATCTGCAGCGATGAACTTTATAAATATGCTAACAGCTGTTGT
AGCAGTTTCTATAATGGGTTATATACCGTTTGAATACATTTGGAACTTTTTGTTGATGTGTACAATACTACCAATTATTT
GTTTTGTACTAATAATCTATGATAAATTTTAA

Upstream 100 bases:

>100_bases
TCAAATAAATCTAAACTTATGTCAATATATGGTGATATTGTAAAATATATTTTTTAATTTACCATATCTCATAGTTAAAT
TTTTATGAAGAGGATTAATT

Downstream 100 bases:

>100_bases
AAAATAATAATCTTTACAAATATTTTGTTAAATTGATTCTTAAGAAACTAAGTTTAGTATTTGTACTTATTCAAAGCTAG
TGGCAGTAAAACGTTGAACT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYGPIAKKYGDVITLRAGMTINV
IGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNSVAAEKAKLALSFATLSFAISLTLATLIGGIISAYTHWYYC
FYVLLIHAVIMLGASFLYENKKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNST
QYSLWISMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLITLMYFVCNFIYPTAS
YLASTAIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLLMCTILPIICFVLIIYDKF

Sequences:

>Translated_383_residues
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYGPIAKKYGDVITLRAGMTINV
IGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNSVAAEKAKLALSFATLSFAISLTLATLIGGIISAYTHWYYC
FYVLLIHAVIMLGASFLYENKKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNST
QYSLWISMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLITLMYFVCNFIYPTAS
YLASTAIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLLMCTILPIICFVLIIYDKF
>Mature_383_residues
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYGPIAKKYGDVITLRAGMTINV
IGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNSVAAEKAKLALSFATLSFAISLTLATLIGGIISAYTHWYYC
FYVLLIHAVIMLGASFLYENKKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNST
QYSLWISMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLITLMYFVCNFIYPTAS
YLASTAIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLLMCTILPIICFVLIIYDKF

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=249, Percent_Identity=21.285140562249, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082312, Length=311, Percent_Identity=22.8295819935691, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42067; Mature: 42067

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYG
CCCEEEEHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
PIAKKYGDVITLRAGMTINVIGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNS
HHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
VAAEKAKLALSFATLSFAISLTLATLIGGIISAYTHWYYCFYVLLIHAVIMLGASFLYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNST
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
QYSLWISMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFVLITLMYFVCNFIYPTASYLASTAIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WNFLLMCTILPIICFVLIIYDKF
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYG
CCCEEEEHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
PIAKKYGDVITLRAGMTINVIGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNS
HHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
VAAEKAKLALSFATLSFAISLTLATLIGGIISAYTHWYYCFYVLLIHAVIMLGASFLYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNST
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
QYSLWISMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFVLITLMYFVCNFIYPTASYLASTAIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WNFLLMCTILPIICFVLIIYDKF
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]