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The map label for this gene is estA [H]

Identifier: 134302636

GI number: 134302636

Start: 1709254

End: 1709907

Strand: Direct

Name: estA [H]

Synonym: FTW_1817

Alternate gene names: 134302636

Gene position: 1709254-1709907 (Clockwise)

Preceding gene: 134302635

Following gene: 134302637

Centisome position: 90.03

GC content: 33.49

Gene sequence:

>654_bases
ATGGAGCCAGCAAAGCAGGCTAGGTTTTGTGTTATATGGCTGCATGGCCTTGGTGCTGATGGACATGATTTTGTAGATAT
TGTTAACTATTTTGATGTTTCATTGGATGAAATCAGATTTATTTTTCCTCATGCTGATATTATACCTGTGACTATAAACA
TGGGGATGCAGATGCGTGCTTGGTATGATATCAAGTCACTTGATGCAAATAGCCTAAATAGAGTAGTTGATGTTGAAGGT
ATAAATAGTTCAATAGCAAAAGTTAATAAATTAATAGATAGTCAAGTCAATCAAGGTATTGCTAGTGAAAATATTATCTT
AGCGGGTTTTTCACAAGGTGGTATAATTGCTACCTATACGGCTATTACATCTCAAAGGAAGCTTGGTGGTATTATGGCTT
TATCTACATATTTGCCAGCATGGGATAATTTTAAAGGTAAAATTACATCTATAAATAAAGGATTACCTATACTAGTTTGC
CATGGTACGGATGATCAGGTATTACCTGAAGTTCTTGGTCATGATTTATCAGATAAACTAAAAGTTAGCGGTTTTGCTAA
TGAATATAAACACTACGTAGGTATGCAGCATTCTGTATGTATGGAAGAAATAAAAGATATCTCAAACTTTATTGCAAAGA
CATTTAAAATATGA

Upstream 100 bases:

>100_bases
TCGGAGGTGTTTAAATTTTTAGATATTAATAAATTTCTTAAACTAATGTATCATTCTACCTAATATTAGTAGATTTAGAA
ATTTTATGAATTACGAACTT

Downstream 100 bases:

>100_bases
AACAAATAACTATAGCTAGTCGAGAAAGTAAACTAGCATTATGGCAGACAAATTTTATCAAAAATCGTATCCAATCAGAG
CTAAACATTCCTTGTGAAAT

Product: phospholipase/carboxylesterase family protein

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MEPAKQARFCVIWLHGLGADGHDFVDIVNYFDVSLDEIRFIFPHADIIPVTINMGMQMRAWYDIKSLDANSLNRVVDVEG
INSSIAKVNKLIDSQVNQGIASENIILAGFSQGGIIATYTAITSQRKLGGIMALSTYLPAWDNFKGKITSINKGLPILVC
HGTDDQVLPEVLGHDLSDKLKVSGFANEYKHYVGMQHSVCMEEIKDISNFIAKTFKI

Sequences:

>Translated_217_residues
MEPAKQARFCVIWLHGLGADGHDFVDIVNYFDVSLDEIRFIFPHADIIPVTINMGMQMRAWYDIKSLDANSLNRVVDVEG
INSSIAKVNKLIDSQVNQGIASENIILAGFSQGGIIATYTAITSQRKLGGIMALSTYLPAWDNFKGKITSINKGLPILVC
HGTDDQVLPEVLGHDLSDKLKVSGFANEYKHYVGMQHSVCMEEIKDISNFIAKTFKI
>Mature_217_residues
MEPAKQARFCVIWLHGLGADGHDFVDIVNYFDVSLDEIRFIFPHADIIPVTINMGMQMRAWYDIKSLDANSLNRVVDVEG
INSSIAKVNKLIDSQVNQGIASENIILAGFSQGGIIATYTAITSQRKLGGIMALSTYLPAWDNFKGKITSINKGLPILVC
HGTDDQVLPEVLGHDLSDKLKVSGFANEYKHYVGMQHSVCMEEIKDISNFIAKTFKI

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=213, Percent_Identity=40.8450704225352, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI9966764, Length=212, Percent_Identity=36.3207547169811, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI20270341, Length=208, Percent_Identity=25.4807692307692, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508057, Length=212, Percent_Identity=39.1509433962264, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6323147, Length=223, Percent_Identity=34.0807174887892, Blast_Score=118, Evalue=8e-28,
Organism=Drosophila melanogaster, GI45553055, Length=206, Percent_Identity=35.4368932038835, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45553057, Length=206, Percent_Identity=35.4368932038835, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21357257, Length=206, Percent_Identity=35.4368932038835, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078179, Length=211, Percent_Identity=26.5402843601896, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24019; Mature: 24019

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPAKQARFCVIWLHGLGADGHDFVDIVNYFDVSLDEIRFIFPHADIIPVTINMGMQMRA
CCCCHHCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHEEEEECCCCEEEEEECCCCEEEE
WYDIKSLDANSLNRVVDVEGINSSIAKVNKLIDSQVNQGIASENIILAGFSQGGIIATYT
EECHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEE
AITSQRKLGGIMALSTYLPAWDNFKGKITSINKGLPILVCHGTDDQVLPEVLGHDLSDKL
HHHCCHHHCHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCE
KVSGFANEYKHYVGMQHSVCMEEIKDISNFIAKTFKI
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEPAKQARFCVIWLHGLGADGHDFVDIVNYFDVSLDEIRFIFPHADIIPVTINMGMQMRA
CCCCHHCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHEEEEECCCCEEEEEECCCCEEEE
WYDIKSLDANSLNRVVDVEGINSSIAKVNKLIDSQVNQGIASENIILAGFSQGGIIATYT
EECHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEE
AITSQRKLGGIMALSTYLPAWDNFKGKITSINKGLPILVCHGTDDQVLPEVLGHDLSDKL
HHHCCHHHCHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCE
KVSGFANEYKHYVGMQHSVCMEEIKDISNFIAKTFKI
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA