Click here to switch to the map view.

The map label for this gene is nfnB [H]

Identifier: 134302603

GI number: 134302603

Start: 1672526

End: 1673212

Strand: Direct

Name: nfnB [H]

Synonym: FTW_1781

Alternate gene names: 134302603

Gene position: 1672526-1673212 (Clockwise)

Preceding gene: 134302598

Following gene: 134302604

Centisome position: 88.1

GC content: 32.61

Gene sequence:

>687_bases
ATGAATATAGTTGACTACGCTAAAAGCCGTTATACAACAAAAGCTTATGATCCAACAAAAAAACTGTCAAATGAACAAAT
ACAGCAAATAAAAGATATCCTTAGATTTACACCATCAAGTGTAAACTCTCAACCTTGGCACTTTATCTTAGCAACTTCTG
ACCAAGCTAAAGCTAAAATTGCTAAAGCAGCTGAAAATATTCATCCCAAAAATGTAACAAACATTAAAGATTGTGCTTTA
GTCATCGTATTTTGCCTAAAAACTGATATTGATGATGAATATCTAGAGCTACTTCTAAATCAAGAGCGTAAAGATGGTAG
ATTCCCTACTTCAGAATTTGAAAATATGCAAAGAGATGTGCGTAGAAAATTTGCTCTAGCATACAAAGATAAACCCCAAG
AGTTACAAATTTGGACAGAAAAACAACTTTATATTGCTCTTGGTAATATTCTATTAGGACTATCGGCCTTAGAGATAAAT
GCTACACCTATGGAAGGTATAGAGCCACATATAATAGATACTGAATTTAATTTAGCAGAAAAGGGACTTAAAACCTCCGT
AATTGTCACTGCTGGCTATAGCTCTGAAGATGATTTTAATGCTAAGCTACCTAAATCAAGACTTCCTGAGGAAAAAATCT
TTACTGAAGTTTATTATATCTATCGGAAATATCTCAATGAAATCTAA

Upstream 100 bases:

>100_bases
ATAATTACAAAAAAATAAAAATATCTCTAGCCTAAAAAACATTGTTGAAAAATTTTTTATAAAATATACTGATTGAAACC
TTTAAGATTTTATTTTTATT

Downstream 100 bases:

>100_bases
TTTAACTAAAATCACATCGCTTGATGATATGCGTAAAGTTTATCATCGTAGAGTTCCTAAAATGTTCGTAGACTATTGTG
AAGCTGGTTCATGGCAACAG

Product: dihydropteridine reductase

Products: 6,7-dihydropteridine; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKIAKAAENIHPKNVTNIKDCAL
VIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDVRRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEIN
ATPMEGIEPHIIDTEFNLAEKGLKTSVIVTAGYSSEDDFNAKLPKSRLPEEKIFTEVYYIYRKYLNEI

Sequences:

>Translated_228_residues
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKIAKAAENIHPKNVTNIKDCAL
VIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDVRRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEIN
ATPMEGIEPHIIDTEFNLAEKGLKTSVIVTAGYSSEDDFNAKLPKSRLPEEKIFTEVYYIYRKYLNEI
>Mature_228_residues
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKIAKAAENIHPKNVTNIKDCAL
VIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDVRRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEIN
ATPMEGIEPHIIDTEFNLAEKGLKTSVIVTAGYSSEDDFNAKLPKSRLPEEKIFTEVYYIYRKYLNEI

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=217, Percent_Identity=45.1612903225806, Blast_Score=206, Evalue=7e-55,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.5.1.34

Molecular weight: Translated: 26316; Mature: 26316

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKI
CCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
AKAAENIHPKNVTNIKDCALVIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDV
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEINATPMEGIEPHIIDTEFNLAE
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCCHHH
KGLKTSVIVTAGYSSEDDFNAKLPKSRLPEEKIFTEVYYIYRKYLNEI
CCCCEEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIVDYAKSRYTTKAYDPTKKLSNEQIQQIKDILRFTPSSVNSQPWHFILATSDQAKAKI
CCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
AKAAENIHPKNVTNIKDCALVIVFCLKTDIDDEYLELLLNQERKDGRFPTSEFENMQRDV
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RRKFALAYKDKPQELQIWTEKQLYIALGNILLGLSALEINATPMEGIEPHIIDTEFNLAE
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCCHHH
KGLKTSVIVTAGYSSEDDFNAKLPKSRLPEEKIFTEVYYIYRKYLNEI
CCCCEEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6,7,8-tetrahydropteridine; NAD+; NADP+

Specific reaction: a 5,6,7,8-tetrahydropteridine + NAD(P)+ = a 6,7-dihydropteridine + NAD(P)H + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609 [H]