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The map label for this gene is folA [H]

Identifier: 134302598

GI number: 134302598

Start: 1663588

End: 1664112

Strand: Direct

Name: folA [H]

Synonym: FTW_1770

Alternate gene names: 134302598

Gene position: 1663588-1664112 (Clockwise)

Preceding gene: 134302559

Following gene: 134302603

Centisome position: 87.63

GC content: 27.81

Gene sequence:

>525_bases
ATGACTAAAAATAGATTTCTCAAAATGATCTCACTAATCGTTGCTTATGATAAAAACTTTGGTATAGGTAAAGAAAATAC
TCTAGCATGGAAACTATCAGAAGATCTTAAGAACTTTAAAAAAATAACAGAAAACAACTATATTGTTATGGGTAGAAAAA
CCTTTGAATCGATTGGTCGCCCACTACCGAATCGTAAAAATATTATTTTAACTAGAGACAAAGATTACAAACAAGATAAA
TGTTTAATAATAAATAGTACTCAGGATATTTTAAATTTTGCTGAATCAAAACCACACTACGAAATATTTATAATCGGTGG
TGCACAGATCTATAAAGAATTTCTAAAATACGCAGATAGATTGTATATAACCGAAGTTGATGCAGAAATGACTGATCTTG
ATGCTTTCTTTCCACAATGGGATAAATCAAAATATAAACGTATTGGTCACAAACAATTCAAAAAAGATGATAAAAATGAG
TTTGACTTTACATTTAGCGTCTTTGAAAAAAATAAAAATTTGTAA

Upstream 100 bases:

>100_bases
TTCTACTCATATGAAGTATAAGCTGTAAAATTTTACCACAGTTAATTTATAAATTACAAAAGGTATTTTATATTTGTTTT
AAATGCTAAAATACATCAAA

Downstream 100 bases:

>100_bases
TTTAGTCAGACCATTTAAGTAGAATTTTGAGTAATCATAATGTAGATTTAAATTACACAGAATATTAAATTTTTTAATCC
AATGTCAGCTCAAGTCTCAG

Product: dihydrofolate reductase type I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGRPLPNRKNIILTRDKDYKQDK
CLIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFKKDDKNE
FDFTFSVFEKNKNL

Sequences:

>Translated_174_residues
MTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGRPLPNRKNIILTRDKDYKQDK
CLIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFKKDDKNE
FDFTFSVFEKNKNL
>Mature_173_residues
TKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGRPLPNRKNIILTRDKDYKQDKC
LIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFKKDDKNEF
DFTFSVFEKNKNL

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=186, Percent_Identity=31.7204301075269, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI28827793, Length=187, Percent_Identity=31.0160427807487, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=37.6543209876543, Blast_Score=117, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=30.0546448087432, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=140, Percent_Identity=32.8571428571429, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI116008026, Length=178, Percent_Identity=30.8988764044944, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647458, Length=178, Percent_Identity=30.8988764044944, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20708; Mature: 20577

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGR
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECHHHHHHCC
PLPNRKNIILTRDKDYKQDKCLIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADR
CCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHE
LYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFKKDDKNEFDFTFSVFEKNKNL
EEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
TKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGR
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECHHHHHHCC
PLPNRKNIILTRDKDYKQDKCLIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADR
CCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHE
LYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFKKDDKNEFDFTFSVFEKNKNL
EEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]