Click here to switch to the map view.

The map label for this gene is pdxT

Identifier: 134302416

GI number: 134302416

Start: 1458168

End: 1458707

Strand: Reverse

Name: pdxT

Synonym: FTW_1553

Alternate gene names: 134302416

Gene position: 1458707-1458168 (Counterclockwise)

Preceding gene: 134302417

Following gene: 134302413

Centisome position: 76.84

GC content: 32.41

Gene sequence:

>540_bases
ATGACACAAAAAGTCGGAGTATTAGCAATTCAAGGAGGCTATCAAAAGCATGCTGATATGTTCAAGTCTTTGGGAGTTGA
GGTTAAGCTAGTTAAATTTAACAATGATTTTGATAGTATTGATAGACTTGTTATTCCGGGTGGCGAGAGTACAACTTTAT
TAAATCTACTTAATAAGCATCAAATTTTTGATAAGCTATATAACTTTTGTAGTAGTAAGCCAGTTTTTGGTACATGTGCT
GGTAGTATAATTTTGTCAAAAGGAGAAGGATATCTAAATTTGCTAGATCTAGAGGTTCAGAGAAATGCTTATGGACGTCA
AGTTGATAGTTTTGTTGCTGATATAAGCTTTAACGATAAAAATATTACGGGTGTTTTTATTAGGGCTCCAAAGTTTATAG
TAGTTGGTAATCAGGTTGACATTTTATCAAAATATCAAAATTCTCCAGTATTACTTAGACAAGCAAATATTCTCGTGAGT
AGTTTTCATCCTGAACTAACTCAGGATCCTACTATACATGAATATTTTCTTGCGATGTAA

Upstream 100 bases:

>100_bases
ATGATGCTAAAATATTAGCTGAAGTTTCTGAAGATCTTGGTGAACCTATGACAGGCATTAATTGCGATTTTGAGAAATTT
TCACAAAGAGGTTGGTAATA

Downstream 100 bases:

>100_bases
AGTATAGTTAATCCGAGAGTATTTTGTTAAACACATAAGAGATAGCATCACAAAATTTTTCAAAACTATTATTCACTTTT
CTAAATATTTTTTTAAAGTT

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MTQKVGVLAIQGGYQKHADMFKSLGVEVKLVKFNNDFDSIDRLVIPGGESTTLLNLLNKHQIFDKLYNFCSSKPVFGTCA
GSIILSKGEGYLNLLDLEVQRNAYGRQVDSFVADISFNDKNITGVFIRAPKFIVVGNQVDILSKYQNSPVLLRQANILVS
SFHPELTQDPTIHEYFLAM

Sequences:

>Translated_179_residues
MTQKVGVLAIQGGYQKHADMFKSLGVEVKLVKFNNDFDSIDRLVIPGGESTTLLNLLNKHQIFDKLYNFCSSKPVFGTCA
GSIILSKGEGYLNLLDLEVQRNAYGRQVDSFVADISFNDKNITGVFIRAPKFIVVGNQVDILSKYQNSPVLLRQANILVS
SFHPELTQDPTIHEYFLAM
>Mature_178_residues
TQKVGVLAIQGGYQKHADMFKSLGVEVKLVKFNNDFDSIDRLVIPGGESTTLLNLLNKHQIFDKLYNFCSSKPVFGTCAG
SIILSKGEGYLNLLDLEVQRNAYGRQVDSFVADISFNDKNITGVFIRAPKFIVVGNQVDILSKYQNSPVLLRQANILVSS
FHPELTQDPTIHEYFLAM

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=204, Percent_Identity=34.8039215686275, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323995, Length=212, Percent_Identity=34.9056603773585, Blast_Score=94, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321048, Length=212, Percent_Identity=34.9056603773585, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_FRAT1 (Q14IU7)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666679.2
- ProteinModelPortal:   Q14IU7
- SMR:   Q14IU7
- STRING:   Q14IU7
- GeneID:   4199394
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0512
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- PhylomeDB:   Q14IU7
- ProtClustDB:   PRK13526
- BioCyc:   FTUL393115:FTF0512-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 19975; Mature: 19844

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 79-79 ACT_SITE 163-163 ACT_SITE 165-165 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKVGVLAIQGGYQKHADMFKSLGVEVKLVKFNNDFDSIDRLVIPGGESTTLLNLLNKH
CCCCEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCHHCCCEEEECCCCCHHHHHHHHHH
QIFDKLYNFCSSKPVFGTCAGSIILSKGEGYLNLLDLEVQRNAYGRQVDSFVADISFNDK
HHHHHHHHHHCCCCCEEECCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHHEEEEECCC
NITGVFIRAPKFIVVGNQVDILSKYQNSPVLLRQANILVSSFHPELTQDPTIHEYFLAM
CEEEEEEECCEEEEECCCCHHHHHCCCCCEEEEECCEEEECCCCCCCCCCCHHHHEECC
>Mature Secondary Structure 
TQKVGVLAIQGGYQKHADMFKSLGVEVKLVKFNNDFDSIDRLVIPGGESTTLLNLLNKH
CCCEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCHHCCCEEEECCCCCHHHHHHHHHH
QIFDKLYNFCSSKPVFGTCAGSIILSKGEGYLNLLDLEVQRNAYGRQVDSFVADISFNDK
HHHHHHHHHHCCCCCEEECCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHHEEEEECCC
NITGVFIRAPKFIVVGNQVDILSKYQNSPVLLRQANILVSSFHPELTQDPTIHEYFLAM
CEEEEEEECCEEEEECCCCHHHHHCCCCCEEEEECCEEEECCCCCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA