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The map label for this gene is ispH [H]

Identifier: 134302265

GI number: 134302265

Start: 1270447

End: 1271403

Strand: Reverse

Name: ispH [H]

Synonym: FTW_1353

Alternate gene names: 134302265

Gene position: 1271403-1270447 (Counterclockwise)

Preceding gene: 134302266

Following gene: 134302264

Centisome position: 66.97

GC content: 36.89

Gene sequence:

>957_bases
GTGAAGATATTACTAGCTAATCCCAGGGGCTTTTGTGCCGGTGTAAGTCGTGCTGTTGAGACTGTAGAGAAAGTTTTAGA
AGTTGAGAAGTCGCCTGTATATGTGCGTCATGAGGTCGTACATAATAAGGTTGTTGTAGATTCTCTTAAGAAAAAGGGTG
TGGTTTTTGTCAAAGAAGTTGATGAAGTACCAGATGACGCGGTATGTATCTTTAGTGCTCATGGAGTTTCTCTAAAGGTT
GAAGAAGCTGCTGCCAAGAAAAATCTAGTACTTTATGATGCAACATGTCCTTTAGTTACTAAGGTACACAGAGGTGTACG
TTTGGCAAGTAATAACGATGCTGAATGTATCTTGATTGGTCATAAAGGCCATCCAGAAGTTCAAGGTACTATGGGGCAGT
ATCGTAGCAAAAAAGGCGCGATTTATCTTATAGAAAGTGAAGAAGATCTTAATAAATTAACAATAAAAGATCCAGATAAT
CTATACTACGCCACTCAGACTACTTTATCAGTAGATGAGACACAGGGGATAATACAGGCTTTAAAAGACAAATATCCAAA
TATTAAGGGACCTAAAAAAGAAGATATTTGTTATGCAACCCAAAACCGTCAAACAGCTATAAAAGCCATGCTTAAACATA
TTGATGTTTTAGTTGTTGTAGGCTCACAAAATAGCTCTAACTCTAATAGACTAAAAGAGCTAGCAACCTTAGAAGGCATA
GATGCTTATCTTGTTGATAATCCTAAAGACGTTGATAAGTTATGGTTTGATAATAAAAAAGTCTGTGGTGTCAGTGCCGG
AGCTTCTGCACCAGAATACTTGGTTCAGCAAATAATTAGTCAAATATCTAAAGTTTGTTCTACAGAGGTTGAGGAGTTTG
AGGGTATTAAAGAAGAAGTCTATTTCCCACTACCAAGACTTTTAAAGCAAAAGATTGGTACAGGTAAGGTAGAATAA

Upstream 100 bases:

>100_bases
GAGCAAGATGTTACCGTAGATTTTAATCATCCATTATCAGGACAGATAATCGTATTTGAAGCTAAAATTTTAGATGTGGC
TGAGAAAGAGGAGGATTTAG

Downstream 100 bases:

>100_bases
TATGCAAAGATCAATTAGAGGCGCTACTACTGTAGATAAAGATAGTAAAGAAAATGTCATAAGTGCAACAAAAGAGCTTT
TGCAACAAATTATCAAGCAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKILLANPRGFCAGVSRAVETVEKVLEVEKSPVYVRHEVVHNKVVVDSLKKKGVVFVKEVDEVPDDAVCIFSAHGVSLKV
EEAAAKKNLVLYDATCPLVTKVHRGVRLASNNDAECILIGHKGHPEVQGTMGQYRSKKGAIYLIESEEDLNKLTIKDPDN
LYYATQTTLSVDETQGIIQALKDKYPNIKGPKKEDICYATQNRQTAIKAMLKHIDVLVVVGSQNSSNSNRLKELATLEGI
DAYLVDNPKDVDKLWFDNKKVCGVSAGASAPEYLVQQIISQISKVCSTEVEEFEGIKEEVYFPLPRLLKQKIGTGKVE

Sequences:

>Translated_318_residues
MKILLANPRGFCAGVSRAVETVEKVLEVEKSPVYVRHEVVHNKVVVDSLKKKGVVFVKEVDEVPDDAVCIFSAHGVSLKV
EEAAAKKNLVLYDATCPLVTKVHRGVRLASNNDAECILIGHKGHPEVQGTMGQYRSKKGAIYLIESEEDLNKLTIKDPDN
LYYATQTTLSVDETQGIIQALKDKYPNIKGPKKEDICYATQNRQTAIKAMLKHIDVLVVVGSQNSSNSNRLKELATLEGI
DAYLVDNPKDVDKLWFDNKKVCGVSAGASAPEYLVQQIISQISKVCSTEVEEFEGIKEEVYFPLPRLLKQKIGTGKVE
>Mature_318_residues
MKILLANPRGFCAGVSRAVETVEKVLEVEKSPVYVRHEVVHNKVVVDSLKKKGVVFVKEVDEVPDDAVCIFSAHGVSLKV
EEAAAKKNLVLYDATCPLVTKVHRGVRLASNNDAECILIGHKGHPEVQGTMGQYRSKKGAIYLIESEEDLNKLTIKDPDN
LYYATQTTLSVDETQGIIQALKDKYPNIKGPKKEDICYATQNRQTAIKAMLKHIDVLVVVGSQNSSNSNRLKELATLEGI
DAYLVDNPKDVDKLWFDNKKVCGVSAGASAPEYLVQQIISQISKVCSTEVEEFEGIKEEVYFPLPRLLKQKIGTGKVE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=312, Percent_Identity=52.8846153846154, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35153; Mature: 35153

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLANPRGFCAGVSRAVETVEKVLEVEKSPVYVRHEVVHNKVVVDSLKKKGVVFVKEV
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCEEEEEEC
DEVPDDAVCIFSAHGVSLKVEEAAAKKNLVLYDATCPLVTKVHRGVRLASNNDAECILIG
CCCCCCEEEEEEECCCEEEEHHHHHHCCEEEEECCCHHHHHHHCCEEEECCCCCCEEEEC
HKGHPEVQGTMGQYRSKKGAIYLIESEEDLNKLTIKDPDNLYYATQTTLSVDETQGIIQA
CCCCCCCCCCHHHHCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEEEEEHHHHHHHHHH
LKDKYPNIKGPKKEDICYATQNRQTAIKAMLKHIDVLVVVGSQNSSNSNRLKELATLEGI
HHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCC
DAYLVDNPKDVDKLWFDNKKVCGVSAGASAPEYLVQQIISQISKVCSTEVEEFEGIKEEV
CEEEECCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YFPLPRLLKQKIGTGKVE
CCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKILLANPRGFCAGVSRAVETVEKVLEVEKSPVYVRHEVVHNKVVVDSLKKKGVVFVKEV
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCEEEEEEC
DEVPDDAVCIFSAHGVSLKVEEAAAKKNLVLYDATCPLVTKVHRGVRLASNNDAECILIG
CCCCCCEEEEEEECCCEEEEHHHHHHCCEEEEECCCHHHHHHHCCEEEECCCCCCEEEEC
HKGHPEVQGTMGQYRSKKGAIYLIESEEDLNKLTIKDPDNLYYATQTTLSVDETQGIIQA
CCCCCCCCCCHHHHCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEEEEEHHHHHHHHHH
LKDKYPNIKGPKKEDICYATQNRQTAIKAMLKHIDVLVVVGSQNSSNSNRLKELATLEGI
HHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCC
DAYLVDNPKDVDKLWFDNKKVCGVSAGASAPEYLVQQIISQISKVCSTEVEEFEGIKEEV
CEEEECCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YFPLPRLLKQKIGTGKVE
CCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA