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The map label for this gene is cpsA1 [H]

Identifier: 134302173

GI number: 134302173

Start: 1168417

End: 1169577

Strand: Direct

Name: cpsA1 [H]

Synonym: FTW_1240

Alternate gene names: 134302173

Gene position: 1168417-1169577 (Clockwise)

Preceding gene: 134302169

Following gene: 134302182

Centisome position: 61.54

GC content: 34.88

Gene sequence:

>1161_bases
ATGATTAGTATATGTGCAAACACAATAAAAGAACTACAAAGGATTCGCCAACACATACACCAATACCCAGAACTTGGTTT
TGATGTATATAATACTGCTGACTTTATCGCTAATGAGTTAGAAAAAATAGGGCTAGAAATTAAAAGAGGAATCGGTAAGA
CAGGTTTAATAGCTGATTTAAAAGTTACAGCTGCCAAAAAAACTATTGCTCTACGAGCAGATATGGATGCGCTACCAATT
CACGAGCAAAATAATTGTGAATATAAATCTAAAATCGCTGGCAAAGCACATATGTGTGGTCATGATGCTCACTGTGCTAT
GGTTATAGTAGCAGCACAGCAATTTGCTGCAAATAAAGACAAACTAAATGTAAATATTAGATTTATATTTCAACCATATG
AAGAAGTTCTGCCAGGAGATGCTCCAGCAATGATTGCAGATGGAGCCATAGATGGAGTTGATGAAATATATGGTATCCAT
GTATTACCAACAATTAATGAAGGTACAATGCAAATATCTACACCTGTTGCTTTAGCTGGTGTTGATTTATTTGAAATAAC
TTTCTATGGTAGAGGCGGACATGCGAGCACACCAATGAAAGCTAATGATCCAATTATTATGGCTTGTCAATTTGTTAACC
AAGTACAAACAATTGTTTCTAGAAATGCAAATAGCTTTGACCCTTTAGTTATATCTGTAACAGCGATAGAAGCAGGTTCA
GCATTTAATGTAATTCCAGATCAAGCCAAACTAAAAGGTGCAATTCGCTACCTAAGCTCTTCTGGGGAAGATATTGCTAA
AAAACGCCTACATGAAATAGCAAATGGTATAGCTACAACCTACCAAGGAGAAGTAGAGATTAACTATTTCCATGGCTATC
CAGAGACTAGAAATGATTCTAATTCTAGTGCTAGAGCAATCAATAGTGCGAAAAAAGCCCTAGGTGATAACAATGTTATT
GAATCTAAATATCCATGGATGGCATCAGAAGATTTCTCATATTTTGCTAAAAAGGTTCCGGCTTGCTATGCATTTTTAGG
AGTTAAAAATGAAGCTAAAGGCTTTACATCTATGGTACATGAACCAAATTTTGATTTAAGTAATGAAGCTATGCTAAATG
GTGTTAAGTATTATGTTAGTCTTATCTCATCTTTTGAATAA

Upstream 100 bases:

>100_bases
TATATACAAGTCTAATCAATATTTGCCAAAAATTCATATACTTAAATTTAAAGATTATGTAGTACCAAATAACTACTTAT
TATGTTTTTAAGGCTAAGTA

Downstream 100 bases:

>100_bases
AAATAAAAAATAGCTAAATCTGACTTCACCAATAATAGATTTACTTTGATTATTCAGTTATATCAAAAACTTTAACATCT
ATAGATAAAGTCTCGCTACC

Product: aminoacylase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MISICANTIKELQRIRQHIHQYPELGFDVYNTADFIANELEKIGLEIKRGIGKTGLIADLKVTAAKKTIALRADMDALPI
HEQNNCEYKSKIAGKAHMCGHDAHCAMVIVAAQQFAANKDKLNVNIRFIFQPYEEVLPGDAPAMIADGAIDGVDEIYGIH
VLPTINEGTMQISTPVALAGVDLFEITFYGRGGHASTPMKANDPIIMACQFVNQVQTIVSRNANSFDPLVISVTAIEAGS
AFNVIPDQAKLKGAIRYLSSSGEDIAKKRLHEIANGIATTYQGEVEINYFHGYPETRNDSNSSARAINSAKKALGDNNVI
ESKYPWMASEDFSYFAKKVPACYAFLGVKNEAKGFTSMVHEPNFDLSNEAMLNGVKYYVSLISSFE

Sequences:

>Translated_386_residues
MISICANTIKELQRIRQHIHQYPELGFDVYNTADFIANELEKIGLEIKRGIGKTGLIADLKVTAAKKTIALRADMDALPI
HEQNNCEYKSKIAGKAHMCGHDAHCAMVIVAAQQFAANKDKLNVNIRFIFQPYEEVLPGDAPAMIADGAIDGVDEIYGIH
VLPTINEGTMQISTPVALAGVDLFEITFYGRGGHASTPMKANDPIIMACQFVNQVQTIVSRNANSFDPLVISVTAIEAGS
AFNVIPDQAKLKGAIRYLSSSGEDIAKKRLHEIANGIATTYQGEVEINYFHGYPETRNDSNSSARAINSAKKALGDNNVI
ESKYPWMASEDFSYFAKKVPACYAFLGVKNEAKGFTSMVHEPNFDLSNEAMLNGVKYYVSLISSFE
>Mature_386_residues
MISICANTIKELQRIRQHIHQYPELGFDVYNTADFIANELEKIGLEIKRGIGKTGLIADLKVTAAKKTIALRADMDALPI
HEQNNCEYKSKIAGKAHMCGHDAHCAMVIVAAQQFAANKDKLNVNIRFIFQPYEEVLPGDAPAMIADGAIDGVDEIYGIH
VLPTINEGTMQISTPVALAGVDLFEITFYGRGGHASTPMKANDPIIMACQFVNQVQTIVSRNANSFDPLVISVTAIEAGS
AFNVIPDQAKLKGAIRYLSSSGEDIAKKRLHEIANGIATTYQGEVEINYFHGYPETRNDSNSSARAINSAKKALGDNNVI
ESKYPWMASEDFSYFAKKVPACYAFLGVKNEAKGFTSMVHEPNFDLSNEAMLNGVKYYVSLISSFE

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=413, Percent_Identity=24.455205811138, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 42282; Mature: 42282

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISICANTIKELQRIRQHIHQYPELGFDVYNTADFIANELEKIGLEIKRGIGKTGLIADL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KVTAAKKTIALRADMDALPIHEQNNCEYKSKIAGKAHMCGHDAHCAMVIVAAQQFAANKD
EEEECCEEEEEEECCCCCCCCCCCCCCHHHHHCCCHHCCCCCCCHHHHHEEHHHHHCCCC
KLNVNIRFIFQPYEEVLPGDAPAMIADGAIDGVDEIYGIHVLPTINEGTMQISTPVALAG
EEEEEEEEEECCHHHHCCCCCCCEEECCCCCCHHHHCCEEEEEECCCCCEEEECCEEEEC
VDLFEITFYGRGGHASTPMKANDPIIMACQFVNQVQTIVSRNANSFDPLVISVTAIEAGS
CEEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCC
AFNVIPDQAKLKGAIRYLSSSGEDIAKKRLHEIANGIATTYQGEVEINYFHGYPETRNDS
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEECCCCCCCCC
NSSARAINSAKKALGDNNVIESKYPWMASEDFSYFAKKVPACYAFLGVKNEAKGFTSMVH
CCHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
EPNFDLSNEAMLNGVKYYVSLISSFE
CCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISICANTIKELQRIRQHIHQYPELGFDVYNTADFIANELEKIGLEIKRGIGKTGLIADL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KVTAAKKTIALRADMDALPIHEQNNCEYKSKIAGKAHMCGHDAHCAMVIVAAQQFAANKD
EEEECCEEEEEEECCCCCCCCCCCCCCHHHHHCCCHHCCCCCCCHHHHHEEHHHHHCCCC
KLNVNIRFIFQPYEEVLPGDAPAMIADGAIDGVDEIYGIHVLPTINEGTMQISTPVALAG
EEEEEEEEEECCHHHHCCCCCCCEEECCCCCCHHHHCCEEEEEECCCCCEEEECCEEEEC
VDLFEITFYGRGGHASTPMKANDPIIMACQFVNQVQTIVSRNANSFDPLVISVTAIEAGS
CEEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCC
AFNVIPDQAKLKGAIRYLSSSGEDIAKKRLHEIANGIATTYQGEVEINYFHGYPETRNDS
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEECCCCCCCCC
NSSARAINSAKKALGDNNVIESKYPWMASEDFSYFAKKVPACYAFLGVKNEAKGFTSMVH
CCHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
EPNFDLSNEAMLNGVKYYVSLISSFE
CCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]