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The map label for this gene is hsdM [H]

Identifier: 134302158

GI number: 134302158

Start: 1146374

End: 1147822

Strand: Reverse

Name: hsdM [H]

Synonym: FTW_1214

Alternate gene names: 134302158

Gene position: 1147822-1146374 (Counterclockwise)

Preceding gene: 134302159

Following gene: 134302155

Centisome position: 60.46

GC content: 33.95

Gene sequence:

>1449_bases
ATGCAGTCAAAGATAAATAGAATAACGGATATATTAAGAAGAGATGATGGAATCAGTGGGGCGATGCACTATACTGAGCA
AGTCAGCTGGATTCTTTTTTTGAAATTCTTGAACGACTATGAAAATGAAAAATCTCTTGAGGCTGAGCTAATAGGTGAAG
ATTATATATTTGTCCTAGATGAAAAATATCGCTGGAATATTTGGGCAGCTCCAAAAGGTGCAGATGGCAAACTTGATGTT
ATCAATGCTGATACAGGCGATGACTTATTAGAGATTGTCAACAAAGAGCTTTTTCCATATCTGAAATCTTTTAAATCTAT
AGATGAAGATGTCAAAAGTCTAAAGTATAAAATTGGTGCGATTTTTGAATTTTTGGATAACCGTATTGCCAGTGGTCATA
CTCTACGTGATGTTATCAATGAGATTGATGAGCTAAATTTTAATAAAAAAGAAGACCTATATCAGCTATCACAAATCTAT
GAAAACTTACTTAAAGAGATGGGTTCTGATGGTGGTAATAGTGGTGAGTTCTATACCCCAAGACCATTAGTTAAGGCTAT
CGTCGATGTGGTTAATCCTCAAGCTGGGCAAACTGTCTATGATCCAGCAGCTGGAACTTGCGGCTTTTTGATAGATGCTT
ATGAGCATATGTATAGCAAAGAGCTATCAACTACACAGTTGAAATTTCTTAATGAAGAGACTTTTTTTGGCAAAGAAAAA
ACACCTCTATCATATGTCATGGGTGTGATGAATATGATCCTACATGGTATAACATCACCAAATATTAACAAGGCAAATAC
ACTTGTCAAAGATATTCGTAGTCTTGAGGAGAAAGATCGTTATGATATCATCCTTGCTAATCCACCATTTGGCGGTAAAG
AAAAAGCAACTATTCAGACTAACTTTCCAATCAAATCAAATGCTACTGAGTTACTATTTTTACAACATATTTACAAAAGC
CTAAAATTAGGTGGTAGATGTGGAGTAGTGGTGCCAGAAGGTGTGCTTTTTCAAACTAATAATGCTTTTAAAAATGTCAA
AAAAGAATTACTAGAAAATTATAATGTTCATACTATAGTTTCACTACCTGCTGGAGTATTTTTGCCATATAGTGGTGTTA
AGACAAATGTGATTTTCTTTGATAGAGAGGGTAGCACTACGGATATTTTCTATTATGAAGTAAACCCACCGTACAAGCTA
ACCAAAAATAAACCAATTCAGTTTGAGCATTTTGCAGAGTTTTTAGAAATTTGGCAATCGCGTAAACTTACTTATAATAG
CTGGATAGTAAATGTTGCAGATATCAAAGATTATGATATATCAGCTAAAAATCCTAACAAAGTTGAAACTATCGAGCATA
AATCACCAACACAGCTAGTCAATGAGATTAAGCAAAATACCGCTGAGATAAATAAGCTAATGAGTGAGATTGAGAGTATT
ATTCAATAA

Upstream 100 bases:

>100_bases
CTTATTAAAAGTTTTGATAATTTTACAATACTTCCTTTTTTAACTATAGGAATAATAGTAATAATAATTTTAATATTTGT
ATTTGGTGGAAAATAGATCT

Downstream 100 bases:

>100_bases
GCATCAATATATGCTGAGCATTTCTGACTCCTAGTTCTGCGGCTTTCTTATAATAGTACATTGCCTTTTCTTTATTATAT
GGTACGCCATTTTGCTCTTC

Product: N-6 DNA methylase family

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MQSKINRITDILRRDDGISGAMHYTEQVSWILFLKFLNDYENEKSLEAELIGEDYIFVLDEKYRWNIWAAPKGADGKLDV
INADTGDDLLEIVNKELFPYLKSFKSIDEDVKSLKYKIGAIFEFLDNRIASGHTLRDVINEIDELNFNKKEDLYQLSQIY
ENLLKEMGSDGGNSGEFYTPRPLVKAIVDVVNPQAGQTVYDPAAGTCGFLIDAYEHMYSKELSTTQLKFLNEETFFGKEK
TPLSYVMGVMNMILHGITSPNINKANTLVKDIRSLEEKDRYDIILANPPFGGKEKATIQTNFPIKSNATELLFLQHIYKS
LKLGGRCGVVVPEGVLFQTNNAFKNVKKELLENYNVHTIVSLPAGVFLPYSGVKTNVIFFDREGSTTDIFYYEVNPPYKL
TKNKPIQFEHFAEFLEIWQSRKLTYNSWIVNVADIKDYDISAKNPNKVETIEHKSPTQLVNEIKQNTAEINKLMSEIESI
IQ

Sequences:

>Translated_482_residues
MQSKINRITDILRRDDGISGAMHYTEQVSWILFLKFLNDYENEKSLEAELIGEDYIFVLDEKYRWNIWAAPKGADGKLDV
INADTGDDLLEIVNKELFPYLKSFKSIDEDVKSLKYKIGAIFEFLDNRIASGHTLRDVINEIDELNFNKKEDLYQLSQIY
ENLLKEMGSDGGNSGEFYTPRPLVKAIVDVVNPQAGQTVYDPAAGTCGFLIDAYEHMYSKELSTTQLKFLNEETFFGKEK
TPLSYVMGVMNMILHGITSPNINKANTLVKDIRSLEEKDRYDIILANPPFGGKEKATIQTNFPIKSNATELLFLQHIYKS
LKLGGRCGVVVPEGVLFQTNNAFKNVKKELLENYNVHTIVSLPAGVFLPYSGVKTNVIFFDREGSTTDIFYYEVNPPYKL
TKNKPIQFEHFAEFLEIWQSRKLTYNSWIVNVADIKDYDISAKNPNKVETIEHKSPTQLVNEIKQNTAEINKLMSEIESI
IQ
>Mature_482_residues
MQSKINRITDILRRDDGISGAMHYTEQVSWILFLKFLNDYENEKSLEAELIGEDYIFVLDEKYRWNIWAAPKGADGKLDV
INADTGDDLLEIVNKELFPYLKSFKSIDEDVKSLKYKIGAIFEFLDNRIASGHTLRDVINEIDELNFNKKEDLYQLSQIY
ENLLKEMGSDGGNSGEFYTPRPLVKAIVDVVNPQAGQTVYDPAAGTCGFLIDAYEHMYSKELSTTQLKFLNEETFFGKEK
TPLSYVMGVMNMILHGITSPNINKANTLVKDIRSLEEKDRYDIILANPPFGGKEKATIQTNFPIKSNATELLFLQHIYKS
LKLGGRCGVVVPEGVLFQTNNAFKNVKKELLENYNVHTIVSLPAGVFLPYSGVKTNVIFFDREGSTTDIFYYEVNPPYKL
TKNKPIQFEHFAEFLEIWQSRKLTYNSWIVNVADIKDYDISAKNPNKVETIEHKSPTQLVNEIKQNTAEINKLMSEIESI
IQ

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=444, Percent_Identity=29.7297297297297, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55081; Mature: 55081

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKINRITDILRRDDGISGAMHYTEQVSWILFLKFLNDYENEKSLEAELIGEDYIFVLD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEC
EKYRWNIWAAPKGADGKLDVINADTGDDLLEIVNKELFPYLKSFKSIDEDVKSLKYKIGA
CCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEFLDNRIASGHTLRDVINEIDELNFNKKEDLYQLSQIYENLLKEMGSDGGNSGEFYTP
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPLVKAIVDVVNPQAGQTVYDPAAGTCGFLIDAYEHMYSKELSTTQLKFLNEETFFGKEK
HHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCCCC
TPLSYVMGVMNMILHGITSPNINKANTLVKDIRSLEEKDRYDIILANPPFGGKEKATIQT
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEE
NFPIKSNATELLFLQHIYKSLKLGGRCGVVVPEGVLFQTNNAFKNVKKELLENYNVHTIV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCHHHHHHHHHHHHCCCEEEEE
SLPAGVFLPYSGVKTNVIFFDREGSTTDIFYYEVNPPYKLTKNKPIQFEHFAEFLEIWQS
ECCCCEEECCCCCEEEEEEEECCCCEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHC
RKLTYNSWIVNVADIKDYDISAKNPNKVETIEHKSPTQLVNEIKQNTAEINKLMSEIESI
CCEEECCEEEEEECCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQ
HC
>Mature Secondary Structure
MQSKINRITDILRRDDGISGAMHYTEQVSWILFLKFLNDYENEKSLEAELIGEDYIFVLD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEC
EKYRWNIWAAPKGADGKLDVINADTGDDLLEIVNKELFPYLKSFKSIDEDVKSLKYKIGA
CCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEFLDNRIASGHTLRDVINEIDELNFNKKEDLYQLSQIYENLLKEMGSDGGNSGEFYTP
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPLVKAIVDVVNPQAGQTVYDPAAGTCGFLIDAYEHMYSKELSTTQLKFLNEETFFGKEK
HHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCCCC
TPLSYVMGVMNMILHGITSPNINKANTLVKDIRSLEEKDRYDIILANPPFGGKEKATIQT
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEE
NFPIKSNATELLFLQHIYKSLKLGGRCGVVVPEGVLFQTNNAFKNVKKELLENYNVHTIV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCHHHHHHHHHHHHCCCEEEEE
SLPAGVFLPYSGVKTNVIFFDREGSTTDIFYYEVNPPYKLTKNKPIQFEHFAEFLEIWQS
ECCCCEEECCCCCEEEEEEEECCCCEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHC
RKLTYNSWIVNVADIKDYDISAKNPNKVETIEHKSPTQLVNEIKQNTAEINKLMSEIESI
CCEEECCEEEEEECCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQ
HC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]