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The map label for this gene is dus1 [H]

Identifier: 134302001

GI number: 134302001

Start: 971027

End: 972010

Strand: Reverse

Name: dus1 [H]

Synonym: FTW_1025

Alternate gene names: 134302001

Gene position: 972010-971027 (Counterclockwise)

Preceding gene: 134302002

Following gene: 134302000

Centisome position: 51.2

GC content: 37.5

Gene sequence:

>984_bases
ATGGACTTTAAAATTGCAGATATAGAAATCGAAAATAATGTTGTACTTGCACCAATGGCGGGTTTCTGTGATAGTGCATT
TCGTACTATATGCAAAGAGCATGGGGCTGGTCTGATATATACGGAGATGGTTAGTAACAAAGCCGTAGTAGAGCATAATT
GGGAAACCATGGAAATGCTCTATATGGAAAATAGTGAAAAGCCACTTGGTATACAGATATTTGGTACAGATATTAATAGT
TTTGTTGGTGCGACAAAATATATCGCAGAGAATACAGAGTGTGATTTTTTAGATATAAATATGGGTTGTCCTATGCCAAA
AGTTGCTAAGAAGTTACAAGCTGGAGCGGCACTTCTAAAAGATGTTAATAGAATCCATGAGATACTAACAGCTGTAGTAA
AAGCAGTACATAAACCAGTAACCGTAAAGATGCGTATTGGTTGGGATGAACAAAATATCAATGCTATAGAGGTTGCTAAA
GCATGTCAAGATGCAGGTGTAAGTGCTATTGCGATACATGGGCGCACGAGAGAGCAAATGTATACTGGTAAGGCAAATTG
GGATATTATCCGCGATGTCAGAAAAGCAGTAGACACAGTAGTAATTGGTAATGGTGATGTGTTTTGTCCACATAGTGCTA
AGGCTATGATGGAGCATACTGGTGTAGACGCTGTGATGGTTGGGCGAGCTTCTCGTGGTAACCCTTGGATATTTAGACAA
ATTGCAGAATATCTAAATACAGGTGAGCTAATACCGCCACCAACCCCAGTTGAGCGTGTTGATGTACTAGAAGAGCATCT
AAGAAGACTTATTAAACTAAAAACTGCCAAAGTAGCAGTTAAAGAAATCCGTACTCATGCAAGCTTTTACCTTCAAGATT
TACCTAATTCAAAAGAGTTTCGTATGAAGTTAAATCAGTTGGAGCATGAACAAGAGATTTTTAAAGTTCTGGAAGAGTAT
AAAAAATCATTATATAAATTTTAA

Upstream 100 bases:

>100_bases
AATCAAGTTGATGAAGTTCAACAAGAGTTTAAAAAATACGCTATAACTTTTAATCAACCAAAGCAGATGGGGCAATGGTT
TTTATTAGATGGGATAAAAA

Downstream 100 bases:

>100_bases
AAATTCTTTTTAGTTTACAGATACGGTAAAATAGATTTTAAGTTTAATTTTCTAAAATCAATCTATATTTATGAGTCTCA
ATTTAAAAGTTGAATTAAAA

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDFKIADIEIENNVVLAPMAGFCDSAFRTICKEHGAGLIYTEMVSNKAVVEHNWETMEMLYMENSEKPLGIQIFGTDINS
FVGATKYIAENTECDFLDINMGCPMPKVAKKLQAGAALLKDVNRIHEILTAVVKAVHKPVTVKMRIGWDEQNINAIEVAK
ACQDAGVSAIAIHGRTREQMYTGKANWDIIRDVRKAVDTVVIGNGDVFCPHSAKAMMEHTGVDAVMVGRASRGNPWIFRQ
IAEYLNTGELIPPPTPVERVDVLEEHLRRLIKLKTAKVAVKEIRTHASFYLQDLPNSKEFRMKLNQLEHEQEIFKVLEEY
KKSLYKF

Sequences:

>Translated_327_residues
MDFKIADIEIENNVVLAPMAGFCDSAFRTICKEHGAGLIYTEMVSNKAVVEHNWETMEMLYMENSEKPLGIQIFGTDINS
FVGATKYIAENTECDFLDINMGCPMPKVAKKLQAGAALLKDVNRIHEILTAVVKAVHKPVTVKMRIGWDEQNINAIEVAK
ACQDAGVSAIAIHGRTREQMYTGKANWDIIRDVRKAVDTVVIGNGDVFCPHSAKAMMEHTGVDAVMVGRASRGNPWIFRQ
IAEYLNTGELIPPPTPVERVDVLEEHLRRLIKLKTAKVAVKEIRTHASFYLQDLPNSKEFRMKLNQLEHEQEIFKVLEEY
KKSLYKF
>Mature_327_residues
MDFKIADIEIENNVVLAPMAGFCDSAFRTICKEHGAGLIYTEMVSNKAVVEHNWETMEMLYMENSEKPLGIQIFGTDINS
FVGATKYIAENTECDFLDINMGCPMPKVAKKLQAGAALLKDVNRIHEILTAVVKAVHKPVTVKMRIGWDEQNINAIEVAK
ACQDAGVSAIAIHGRTREQMYTGKANWDIIRDVRKAVDTVVIGNGDVFCPHSAKAMMEHTGVDAVMVGRASRGNPWIFRQ
IAEYLNTGELIPPPTPVERVDVLEEHLRRLIKLKTAKVAVKEIRTHASFYLQDLPNSKEFRMKLNQLEHEQEIFKVLEEY
KKSLYKF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=30.9623430962343, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=31.2236286919831, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=32.4561403508772, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=29.7959183673469, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=27.7533039647577, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=40.809968847352, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1788462, Length=317, Percent_Identity=29.6529968454259, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI145693211, Length=322, Percent_Identity=24.223602484472, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=317, Percent_Identity=29.9684542586751, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=30.4166666666667, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17510279, Length=251, Percent_Identity=31.8725099601594, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=332, Percent_Identity=28.9156626506024, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=25.9911894273128, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=289, Percent_Identity=24.9134948096886, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585320, Length=243, Percent_Identity=33.7448559670782, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=31.4741035856574, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=31.4741035856574, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45549423, Length=328, Percent_Identity=26.5243902439024, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.3842794759825, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36893; Mature: 36893

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKIADIEIENNVVLAPMAGFCDSAFRTICKEHGAGLIYTEMVSNKAVVEHNWETMEML
CCCEEEEEEEECCEEEECCHHHHHHHHHHHHHHHCCCEEEEHHHCCCEEEECCCCCEEEE
YMENSEKPLGIQIFGTDINSFVGATKYIAENTECDFLDINMGCPMPKVAKKLQAGAALLK
EECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
DVNRIHEILTAVVKAVHKPVTVKMRIGWDEQNINAIEVAKACQDAGVSAIAIHGRTREQM
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHH
YTGKANWDIIRDVRKAVDTVVIGNGDVFCPHSAKAMMEHTGVDAVMVGRASRGNPWIFRQ
CCCCCCHHHHHHHHHHHCEEEECCCCEECCCHHHHHHHHCCCCEEEECCCCCCCCHHHHH
IAEYLNTGELIPPPTPVERVDVLEEHLRRLIKLKTAKVAVKEIRTHASFYLQDLPNSKEF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RMKLNQLEHEQEIFKVLEEYKKSLYKF
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFKIADIEIENNVVLAPMAGFCDSAFRTICKEHGAGLIYTEMVSNKAVVEHNWETMEML
CCCEEEEEEEECCEEEECCHHHHHHHHHHHHHHHCCCEEEEHHHCCCEEEECCCCCEEEE
YMENSEKPLGIQIFGTDINSFVGATKYIAENTECDFLDINMGCPMPKVAKKLQAGAALLK
EECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
DVNRIHEILTAVVKAVHKPVTVKMRIGWDEQNINAIEVAKACQDAGVSAIAIHGRTREQM
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHH
YTGKANWDIIRDVRKAVDTVVIGNGDVFCPHSAKAMMEHTGVDAVMVGRASRGNPWIFRQ
CCCCCCHHHHHHHHHHHCEEEECCCCEECCCHHHHHHHHCCCCEEEECCCCCCCCHHHHH
IAEYLNTGELIPPPTPVERVDVLEEHLRRLIKLKTAKVAVKEIRTHASFYLQDLPNSKEF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RMKLNQLEHEQEIFKVLEEYKKSLYKF
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]