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The map label for this gene is rsmE [H]

Identifier: 134301732

GI number: 134301732

Start: 672402

End: 673130

Strand: Reverse

Name: rsmE [H]

Synonym: FTW_0692

Alternate gene names: 134301732

Gene position: 673130-672402 (Counterclockwise)

Preceding gene: 134301734

Following gene: 134301731

Centisome position: 35.46

GC content: 27.02

Gene sequence:

>729_bases
ATGAGAACTATTCGAGGTTTTTTCTCTGACATTTCAACAAAGACTACAATTTTTAATATTTCAGGAGAATATTATAACTA
TTTCAAAAATGTGTTACGCTTAAAAAAATCTGATTTGATTGAACTATTCAATAATACTGATAGCTTACAATATAAGACTA
AAATTATCACGATTGATAATAAAAATATAACTCTTGAAGTAATCGATAAAAAACACATCAGTAATGAAAATAACTATATT
ACAAACCTCTATCAGGCTATTATTAAAAGTGAAAATTTTGAGCTTGTGATTCAAAAAGCTACCGAACTAGGGGTAAATAA
CATATATCCTATAATTACTGAATTTACAAATCACAAGTTTGATAAAAATGGCTTTGACAAAAAGCTAGAGCGCTGGCATA
AAATTGCTATTGCTGGCAGTGAACAATGTGGTCGTGTTTTTATTCCAAAAATTCATACTCCTATCGATATTAATGATTTA
CAAATGCCTCAAAATGACCTTAATTTAGTTTTATGTCCATATACTCAAGCAAAACAAGATTTTGTACAAAAGGTTAAATC
TAGTACAAATTTTAATATATTTATAGGACCAGAAGGTGGCTTTAGTGATAAAGAAATGAGCAAATTCCATAGCAATAATT
TTTATACAATTAATTTGGGCAAAAGAATACTTAAAGCAGAAACTGCTCCAATAAATATATTATCAATTATAAACTTTCTT
AAACAGTAA

Upstream 100 bases:

>100_bases
AGTTACAAAACTTTTATTAAAAATACCCATAAACTTTTCCTACTTTTTAAAAATTAAGAATTTATAATAAGAGATATGTA
TTTTACCAAATTTTAATTTT

Downstream 100 bases:

>100_bases
GTTTTACCAAGGGAATTGAGCTAATTAATAAAGCTTGATAAAACAAATTAACTTAGCACAGTATTAGTCTAGCTTCCAAA
ATTGTACAACAAGAGGTTAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRTIRGFFSDISTKTTIFNISGEYYNYFKNVLRLKKSDLIELFNNTDSLQYKTKIITIDNKNITLEVIDKKHISNENNYI
TNLYQAIIKSENFELVIQKATELGVNNIYPIITEFTNHKFDKNGFDKKLERWHKIAIAGSEQCGRVFIPKIHTPIDINDL
QMPQNDLNLVLCPYTQAKQDFVQKVKSSTNFNIFIGPEGGFSDKEMSKFHSNNFYTINLGKRILKAETAPINILSIINFL
KQ

Sequences:

>Translated_242_residues
MRTIRGFFSDISTKTTIFNISGEYYNYFKNVLRLKKSDLIELFNNTDSLQYKTKIITIDNKNITLEVIDKKHISNENNYI
TNLYQAIIKSENFELVIQKATELGVNNIYPIITEFTNHKFDKNGFDKKLERWHKIAIAGSEQCGRVFIPKIHTPIDINDL
QMPQNDLNLVLCPYTQAKQDFVQKVKSSTNFNIFIGPEGGFSDKEMSKFHSNNFYTINLGKRILKAETAPINILSIINFL
KQ
>Mature_242_residues
MRTIRGFFSDISTKTTIFNISGEYYNYFKNVLRLKKSDLIELFNNTDSLQYKTKIITIDNKNITLEVIDKKHISNENNYI
TNLYQAIIKSENFELVIQKATELGVNNIYPIITEFTNHKFDKNGFDKKLERWHKIAIAGSEQCGRVFIPKIHTPIDINDL
QMPQNDLNLVLCPYTQAKQDFVQKVKSSTNFNIFIGPEGGFSDKEMSKFHSNNFYTINLGKRILKAETAPINILSIINFL
KQ

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=239, Percent_Identity=28.0334728033473, Blast_Score=117, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28047; Mature: 28047

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIRGFFSDISTKTTIFNISGEYYNYFKNVLRLKKSDLIELFNNTDSLQYKTKIITIDN
CCCHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEC
KNITLEVIDKKHISNENNYITNLYQAIIKSENFELVIQKATELGVNNIYPIITEFTNHKF
CEEEEEEECCHHCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHCCCCC
DKNGFDKKLERWHKIAIAGSEQCGRVFIPKIHTPIDINDLQMPQNDLNLVLCPYTQAKQD
CCCCHHHHHHHHHEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHH
FVQKVKSSTNFNIFIGPEGGFSDKEMSKFHSNNFYTINLGKRILKAETAPINILSIINFL
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCHHHHCCCCCHHHHHHHHHH
KQ
CC
>Mature Secondary Structure
MRTIRGFFSDISTKTTIFNISGEYYNYFKNVLRLKKSDLIELFNNTDSLQYKTKIITIDN
CCCHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEC
KNITLEVIDKKHISNENNYITNLYQAIIKSENFELVIQKATELGVNNIYPIITEFTNHKF
CEEEEEEECCHHCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHCCCCC
DKNGFDKKLERWHKIAIAGSEQCGRVFIPKIHTPIDINDLQMPQNDLNLVLCPYTQAKQD
CCCCHHHHHHHHHEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHH
FVQKVKSSTNFNIFIGPEGGFSDKEMSKFHSNNFYTINLGKRILKAETAPINILSIINFL
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCHHHHCCCCCHHHHHHHHHH
KQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]