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The map label for this gene is nadB [H]

Identifier: 134301690

GI number: 134301690

Start: 622898

End: 624385

Strand: Direct

Name: nadB [H]

Synonym: FTW_0631

Alternate gene names: 134301690

Gene position: 622898-624385 (Clockwise)

Preceding gene: 134301689

Following gene: 134301692

Centisome position: 32.81

GC content: 34.14

Gene sequence:

>1488_bases
ATGATTATAAAAAAGCATGATGTAGCTATAATAGGTGGTGGTTTAGCAGGTGTAGTTGCTGCTATTGAGTTAGCTGAGAA
TAATTTAGATGTTGCAATCATCTATGACCAAAAAATCAATCATTGTGCTAGTGCATATGCTCAAGGTGGCATTGCGGCTA
TAGTTTCTAGTGATGATAGCATTGAGGCTCATATTAATGATACATATATTGCTAGTGGTAAGCTTGCAAAGCTTGAGAGT
ATTACTCAAGTTGTTACAAATTCTAATGCTGCTATAGCTTGGCTTGAGAAACATGGTGTTGAGTTTGATAAAAAAGAAAA
TGGTCAGTATAGCCTACATCTTGAAGGCGGACACTCGCAAGCAAGGATTCTGCATATCAAAGACTATACTGGTAGAGCAG
TTATTACAAGTTTATATAAAAATCTTGATAGTTTTAAAAATATCAGTACTTATCCAGAACATAATGTCATAGAACTAATA
AAAAAAGATTATAAATGTGTTGGTCTATATAGTCATGATAATAAATATCAGGTTACTAAATTTATAACCAAGAAAGTCAT
ACTTGCTGCAGGTGGGGCATCAGGATTATATAAGTATGTGACTAATGCTACAGCAGGTAATGGCAGTGCGATGATTATGG
CTTATGATATTGGTTGCCAACTTGAGAATTTAGAGTTTACGCAATTTCATCCAACTTGTTTTTTTGCCCGCAATGGAGCG
CCTCTTTTAATCTCAGAAGCTATCAGGGGTAGTGGGGCTGTGCTTGAGACTGTAAAAGGTGTCAGGATAATGAAATCAAT
CCACGAGAAACAAGATTTAGCCCCTAGAGATATAGTTGCTCGAGAGATATATATAAATATTCAAGCTGGTAGAGATGTTT
ACCTTAATGCGACACATCTAAACACAAGTCAGTGGCAGCAAAAATTTCCATATATTTATGAGAAATTATTAGAAAATAAT
ATCAATCCAGTTAATGATAGAATTCCCGTATCTCCAGCAGCACACTATAGTTGCGGCGGTATTAGTGTAGCTAATAATTC
TCAAACTAATATTGCTAATCTCTACGCTGTAGGTGAGGTTGCTTGTACTGGTTTACATGGTGCTAATAGGTTAGCAAGTA
ACTCATTACTAGAATGTGTTGTGTATGCTTTAGCAGCTAGTCAGGATATTTTAGCTAATATAAATGATAGCTTTACTGAA
TGTGATCAAAAATTAGAGTTATTTAACTCAAATAATAATTATATACAACAGATTAATCAGATACGCCAATTAATGTGGGA
TAATGTTGGTTTGGTTAGAAGACAAGCAGAACTTAGTAAAGCATATCAGCAATTACAACTATTAGCTCAATCAATAAGTA
CTGATACAAGACTAGCAAACTATGATTATAAACTTGAAGCTTATAGGAAAATACTTAAGCTAGCTACGTTAATACTTGAA
CAAGCTATCAAACGCAAAGACAGTGTTGGTAGTCATTTTTTAATTTAA

Upstream 100 bases:

>100_bases
TAGTTGCTATAGCTAAAACTGGAGTTGATTTTATTTCTGTTGGGGCGATTACAAAACATATCAAAGCAATAGATTTATCA
TTGCAGGTTCAGTTATAAAT

Downstream 100 bases:

>100_bases
AGGCAGATAGATATTAATATGAGAGATAAGATTTTTTCGATACCAAAAGTTGTTTTGCATGATCATCTTGATGGTGGTTT
GCGTGTTGATACTATTATAG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MIIKKHDVAIIGGGLAGVVAAIELAENNLDVAIIYDQKINHCASAYAQGGIAAIVSSDDSIEAHINDTYIASGKLAKLES
ITQVVTNSNAAIAWLEKHGVEFDKKENGQYSLHLEGGHSQARILHIKDYTGRAVITSLYKNLDSFKNISTYPEHNVIELI
KKDYKCVGLYSHDNKYQVTKFITKKVILAAGGASGLYKYVTNATAGNGSAMIMAYDIGCQLENLEFTQFHPTCFFARNGA
PLLISEAIRGSGAVLETVKGVRIMKSIHEKQDLAPRDIVAREIYINIQAGRDVYLNATHLNTSQWQQKFPYIYEKLLENN
INPVNDRIPVSPAAHYSCGGISVANNSQTNIANLYAVGEVACTGLHGANRLASNSLLECVVYALAASQDILANINDSFTE
CDQKLELFNSNNNYIQQINQIRQLMWDNVGLVRRQAELSKAYQQLQLLAQSISTDTRLANYDYKLEAYRKILKLATLILE
QAIKRKDSVGSHFLI

Sequences:

>Translated_495_residues
MIIKKHDVAIIGGGLAGVVAAIELAENNLDVAIIYDQKINHCASAYAQGGIAAIVSSDDSIEAHINDTYIASGKLAKLES
ITQVVTNSNAAIAWLEKHGVEFDKKENGQYSLHLEGGHSQARILHIKDYTGRAVITSLYKNLDSFKNISTYPEHNVIELI
KKDYKCVGLYSHDNKYQVTKFITKKVILAAGGASGLYKYVTNATAGNGSAMIMAYDIGCQLENLEFTQFHPTCFFARNGA
PLLISEAIRGSGAVLETVKGVRIMKSIHEKQDLAPRDIVAREIYINIQAGRDVYLNATHLNTSQWQQKFPYIYEKLLENN
INPVNDRIPVSPAAHYSCGGISVANNSQTNIANLYAVGEVACTGLHGANRLASNSLLECVVYALAASQDILANINDSFTE
CDQKLELFNSNNNYIQQINQIRQLMWDNVGLVRRQAELSKAYQQLQLLAQSISTDTRLANYDYKLEAYRKILKLATLILE
QAIKRKDSVGSHFLI
>Mature_495_residues
MIIKKHDVAIIGGGLAGVVAAIELAENNLDVAIIYDQKINHCASAYAQGGIAAIVSSDDSIEAHINDTYIASGKLAKLES
ITQVVTNSNAAIAWLEKHGVEFDKKENGQYSLHLEGGHSQARILHIKDYTGRAVITSLYKNLDSFKNISTYPEHNVIELI
KKDYKCVGLYSHDNKYQVTKFITKKVILAAGGASGLYKYVTNATAGNGSAMIMAYDIGCQLENLEFTQFHPTCFFARNGA
PLLISEAIRGSGAVLETVKGVRIMKSIHEKQDLAPRDIVAREIYINIQAGRDVYLNATHLNTSQWQQKFPYIYEKLLENN
INPVNDRIPVSPAAHYSCGGISVANNSQTNIANLYAVGEVACTGLHGANRLASNSLLECVVYALAASQDILANINDSFTE
CDQKLELFNSNNNYIQQINQIRQLMWDNVGLVRRQAELSKAYQQLQLLAQSISTDTRLANYDYKLEAYRKILKLATLILE
QAIKRKDSVGSHFLI

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=400, Percent_Identity=31.25, Blast_Score=169, Evalue=7e-42,
Organism=Escherichia coli, GI1788928, Length=513, Percent_Identity=38.7914230019493, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI1790597, Length=489, Percent_Identity=29.038854805726, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1786942, Length=524, Percent_Identity=28.4351145038168, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17550100, Length=402, Percent_Identity=30.8457711442786, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17505833, Length=417, Percent_Identity=30.2158273381295, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71986328, Length=433, Percent_Identity=25.635103926097, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322701, Length=543, Percent_Identity=27.8084714548803, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322416, Length=547, Percent_Identity=27.7879341864717, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24663005, Length=428, Percent_Identity=29.9065420560748, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17137288, Length=374, Percent_Identity=31.0160427807487, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24655642, Length=374, Percent_Identity=31.0160427807487, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24655647, Length=374, Percent_Identity=31.0160427807487, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 54649; Mature: 54649

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKKHDVAIIGGGLAGVVAAIELAENNLDVAIIYDQKINHCASAYAQGGIAAIVSSDDS
CEEEECCEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCC
IEAHINDTYIASGKLAKLESITQVVTNSNAAIAWLEKHGVEFDKKENGQYSLHLEGGHSQ
EEEEECCEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCEEEEEEECCCCC
ARILHIKDYTGRAVITSLYKNLDSFKNISTYPEHNVIELIKKDYKCVGLYSHDNKYQVTK
EEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHH
FITKKVILAAGGASGLYKYVTNATAGNGSAMIMAYDIGCQLENLEFTQFHPTCFFARNGA
HHHHHHEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCEEECCEEEEECCEEEEECCCC
PLLISEAIRGSGAVLETVKGVRIMKSIHEKQDLAPRDIVAREIYINIQAGRDVYLNATHL
CEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEEECCCEEEEEEEEC
NTSQWQQKFPYIYEKLLENNINPVNDRIPVSPAAHYSCGGISVANNSQTNIANLYAVGEV
CHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHEEEEHHH
ACTGLHGANRLASNSLLECVVYALAASQDILANINDSFTECDQKLELFNSNNNYIQQINQ
HEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
IRQLMWDNVGLVRRQAELSKAYQQLQLLAQSISTDTRLANYDYKLEAYRKILKLATLILE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
QAIKRKDSVGSHFLI
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MIIKKHDVAIIGGGLAGVVAAIELAENNLDVAIIYDQKINHCASAYAQGGIAAIVSSDDS
CEEEECCEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCC
IEAHINDTYIASGKLAKLESITQVVTNSNAAIAWLEKHGVEFDKKENGQYSLHLEGGHSQ
EEEEECCEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCEEEEEEECCCCC
ARILHIKDYTGRAVITSLYKNLDSFKNISTYPEHNVIELIKKDYKCVGLYSHDNKYQVTK
EEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHH
FITKKVILAAGGASGLYKYVTNATAGNGSAMIMAYDIGCQLENLEFTQFHPTCFFARNGA
HHHHHHEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCEEECCEEEEECCEEEEECCCC
PLLISEAIRGSGAVLETVKGVRIMKSIHEKQDLAPRDIVAREIYINIQAGRDVYLNATHL
CEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEEECCCEEEEEEEEC
NTSQWQQKFPYIYEKLLENNINPVNDRIPVSPAAHYSCGGISVANNSQTNIANLYAVGEV
CHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHEEEEHHH
ACTGLHGANRLASNSLLECVVYALAASQDILANINDSFTECDQKLELFNSNNNYIQQINQ
HEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
IRQLMWDNVGLVRRQAELSKAYQQLQLLAQSISTDTRLANYDYKLEAYRKILKLATLILE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
QAIKRKDSVGSHFLI
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]