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The map label for this gene is wcaJ [H]

Identifier: 134301647

GI number: 134301647

Start: 578699

End: 580069

Strand: Direct

Name: wcaJ [H]

Synonym: FTW_0587

Alternate gene names: 134301647

Gene position: 578699-580069 (Clockwise)

Preceding gene: 134301646

Following gene: 134301648

Centisome position: 30.48

GC content: 32.46

Gene sequence:

>1371_bases
GTGATCATTCTCAAAACAATAGAGCTAGTAATATCTGTAATACTTGTATTACTAAGTTACTCTATACCTTTGCATCTACT
AAATTATGATACTAGTAATTTTAGTAGTTTTGATTTTATGGCTACTTTTATAGTACTAACATCTTTAACAACTTTTCTTA
TCGCTGAATATACTAATGGTGAAAAAGTATCTAAATCAAAAAATGTTCTAAAAGTTTTATTATGTGGACTAGCTATTGCA
ATTATCATAACATCGCTAGCATTCTTTCTAAGAGGCTTTTCATTTCCACGTAGCTTGATTATTTTGGGTTTTTTGCTGCA
GCTTGTAATGCTATCTGTATCTCGTAATATTTTTAGGTGGCTTATAAGAAATACTAGCTATAGCAGAATCCTAATTGTTG
GTTTAGATCAGGAAAGAGAGTGGCTTTTTGCTAAAGCTGAGGCTGCTAAGTTACCTAGGGAAATAATTGCTGGATACTTG
AGTATAAATATCGATGGTTTTAGTCTAGCTGATGTGGCATATAGTTATAAAAAGGCATTTATCTCAGATAAAGCGCTGAA
GCTATTAGCTGATAATGATTTATCAACTTTAAGTAAATATAATCTTGAAGTGGTTCTTATCCCAAGAAAGTATGAAATAT
CAATTTGGGGGGCGGCGCTAGTGCCTCTTGGTGATAGCTTAGCTATGAGTGTTAAAAATTTTGGTATATCATATGAAGCA
AAAATTATCAAAAGAATTTTTGATGTGCTGTTTAGTTTAATTATCATTGTTCTAACAGCTCCAATTATGCTAATAGTTGC
TTTGGCGATTTACCTTGAAGATAGAGAATCACCATTTTTTATCCAAGAGCGTGTTACAAGAAATGCAAAAAGATTTAATC
TAATCAAGTTTCGCAGTATGAAAGTAAATGCTGAAGTACAAACAGGCGCGGTTTGGGCTGTTGATGGTGATACTAGAATT
ACAAAAGTTGGTAAAATAATTCGCCCAATATGGTTAGATGAGTTGCCGCAGTTTTTTAATGTTTTAAAAGGTGATATGTC
AATAGTAGGGCCGCGGCCAGAGCGACCAGAGCTGATAGACAAATTTAGTCAACAAATTCCTGAGTTTTCTTATAGGACAA
AAGTTAAGGCGGGTATTACTGGTTATGCGCAAGTTTTGACAAGTTATGCAACTCTACCAGAAAATAAGTTAAAACTTGAT
CTTGTCTATATCAGAAGATGGAGTTTTGTTTTTGATTTGCTTATCATTATAGAAACTGTGAGAGTTATTGCTATGAAAAT
TTTGCGACTATTTATAAAAACTAAAGAGCAATCTCAAGCAAGTTTTGTAGAGAAAAAACACAAAAACTATACAGAGTATA
TTTATGAATAA

Upstream 100 bases:

>100_bases
GGAGTAAATGCCTTTGTTGCTGGTTCAGCTATTTTTAATTCTGATAGCTATAAGCAAACTATTGATAAGATGAGAGATGA
GCTCAATAAAGTCTAATTTA

Downstream 100 bases:

>100_bases
AAAAATCTTAGTAACAGGTGGTGTAGGCTATATAGGTAGTCATACAGTGGTAGAACTTCTTGATAGAGATTATCAAGTTG
TGGTGGTAGATAATCTTTCA

Product: sugar transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MIILKTIELVISVILVLLSYSIPLHLLNYDTSNFSSFDFMATFIVLTSLTTFLIAEYTNGEKVSKSKNVLKVLLCGLAIA
IIITSLAFFLRGFSFPRSLIILGFLLQLVMLSVSRNIFRWLIRNTSYSRILIVGLDQEREWLFAKAEAAKLPREIIAGYL
SINIDGFSLADVAYSYKKAFISDKALKLLADNDLSTLSKYNLEVVLIPRKYEISIWGAALVPLGDSLAMSVKNFGISYEA
KIIKRIFDVLFSLIIIVLTAPIMLIVALAIYLEDRESPFFIQERVTRNAKRFNLIKFRSMKVNAEVQTGAVWAVDGDTRI
TKVGKIIRPIWLDELPQFFNVLKGDMSIVGPRPERPELIDKFSQQIPEFSYRTKVKAGITGYAQVLTSYATLPENKLKLD
LVYIRRWSFVFDLLIIIETVRVIAMKILRLFIKTKEQSQASFVEKKHKNYTEYIYE

Sequences:

>Translated_456_residues
MIILKTIELVISVILVLLSYSIPLHLLNYDTSNFSSFDFMATFIVLTSLTTFLIAEYTNGEKVSKSKNVLKVLLCGLAIA
IIITSLAFFLRGFSFPRSLIILGFLLQLVMLSVSRNIFRWLIRNTSYSRILIVGLDQEREWLFAKAEAAKLPREIIAGYL
SINIDGFSLADVAYSYKKAFISDKALKLLADNDLSTLSKYNLEVVLIPRKYEISIWGAALVPLGDSLAMSVKNFGISYEA
KIIKRIFDVLFSLIIIVLTAPIMLIVALAIYLEDRESPFFIQERVTRNAKRFNLIKFRSMKVNAEVQTGAVWAVDGDTRI
TKVGKIIRPIWLDELPQFFNVLKGDMSIVGPRPERPELIDKFSQQIPEFSYRTKVKAGITGYAQVLTSYATLPENKLKLD
LVYIRRWSFVFDLLIIIETVRVIAMKILRLFIKTKEQSQASFVEKKHKNYTEYIYE
>Mature_456_residues
MIILKTIELVISVILVLLSYSIPLHLLNYDTSNFSSFDFMATFIVLTSLTTFLIAEYTNGEKVSKSKNVLKVLLCGLAIA
IIITSLAFFLRGFSFPRSLIILGFLLQLVMLSVSRNIFRWLIRNTSYSRILIVGLDQEREWLFAKAEAAKLPREIIAGYL
SINIDGFSLADVAYSYKKAFISDKALKLLADNDLSTLSKYNLEVVLIPRKYEISIWGAALVPLGDSLAMSVKNFGISYEA
KIIKRIFDVLFSLIIIVLTAPIMLIVALAIYLEDRESPFFIQERVTRNAKRFNLIKFRSMKVNAEVQTGAVWAVDGDTRI
TKVGKIIRPIWLDELPQFFNVLKGDMSIVGPRPERPELIDKFSQQIPEFSYRTKVKAGITGYAQVLTSYATLPENKLKLD
LVYIRRWSFVFDLLIIIETVRVIAMKILRLFIKTKEQSQASFVEKKHKNYTEYIYE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=417, Percent_Identity=29.2565947242206, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52087; Mature: 52087

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILKTIELVISVILVLLSYSIPLHLLNYDTSNFSSFDFMATFIVLTSLTTFLIAEYTNG
CEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
EKVSKSKNVLKVLLCGLAIAIIITSLAFFLRGFSFPRSLIILGFLLQLVMLSVSRNIFRW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIRNTSYSRILIVGLDQEREWLFAKAEAAKLPREIIAGYLSINIDGFSLADVAYSYKKAF
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHEEEECCCHHHHHHHHHHHHH
ISDKALKLLADNDLSTLSKYNLEVVLIPRKYEISIWGAALVPLGDSLAMSVKNFGISYEA
HHHHHHHHHCCCCHHHHHHCCEEEEEECCEEEEEEECEEEECCCCHHHHHHHHCCCCHHH
KIIKRIFDVLFSLIIIVLTAPIMLIVALAIYLEDRESPFFIQERVTRNAKRFNLIKFRSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHEEEEEEEEE
KVNAEVQTGAVWAVDGDTRITKVGKIIRPIWLDELPQFFNVLKGDMSIVGPRPERPELID
EECCEEEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
KFSQQIPEFSYRTKVKAGITGYAQVLTSYATLPENKLKLDLVYIRRWSFVFDLLIIIETV
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RVIAMKILRLFIKTKEQSQASFVEKKHKNYTEYIYE
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MIILKTIELVISVILVLLSYSIPLHLLNYDTSNFSSFDFMATFIVLTSLTTFLIAEYTNG
CEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
EKVSKSKNVLKVLLCGLAIAIIITSLAFFLRGFSFPRSLIILGFLLQLVMLSVSRNIFRW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIRNTSYSRILIVGLDQEREWLFAKAEAAKLPREIIAGYLSINIDGFSLADVAYSYKKAF
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHEEEECCCHHHHHHHHHHHHH
ISDKALKLLADNDLSTLSKYNLEVVLIPRKYEISIWGAALVPLGDSLAMSVKNFGISYEA
HHHHHHHHHCCCCHHHHHHCCEEEEEECCEEEEEEECEEEECCCCHHHHHHHHCCCCHHH
KIIKRIFDVLFSLIIIVLTAPIMLIVALAIYLEDRESPFFIQERVTRNAKRFNLIKFRSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHEEEEEEEEE
KVNAEVQTGAVWAVDGDTRITKVGKIIRPIWLDELPQFFNVLKGDMSIVGPRPERPELID
EECCEEEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
KFSQQIPEFSYRTKVKAGITGYAQVLTSYATLPENKLKLDLVYIRRWSFVFDLLIIIETV
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RVIAMKILRLFIKTKEQSQASFVEKKHKNYTEYIYE
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]