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The map label for this gene is proS [H]

Identifier: 134301543

GI number: 134301543

Start: 460458

End: 462155

Strand: Reverse

Name: proS [H]

Synonym: FTW_0472

Alternate gene names: 134301543

Gene position: 462155-460458 (Counterclockwise)

Preceding gene: 134301544

Following gene: 134301542

Centisome position: 24.34

GC content: 35.51

Gene sequence:

>1698_bases
ATGAAAGCAACACAAACTCTAATTGCTACGACAAAGGAACTACCAAAAGAAGCAGTTCTAATTAGTCATCAATATATGCT
CAAAGCTGGTCTTATCAAGAAGCTAGCTTCTGGTATCTATACATGGATGCCTCTAGGTCTAAAGGTACTCCAAAAAATCC
AAAATATAGTACGTGATGAGATGAATAAAGCTGGAGCAAGCGAACTACTTTTACCAAGTATACTACCTTCAGAGCTTTTA
CAAGAAACTCATCGCTGGGATAAATTTGGTCCAGAACTTTTAAAATTACATGATCGACATAATAGAGATTTCTGTTATGG
TCCAACACATGAAGAACCAATTGTTGATATGGCTAGAGATACTATCAAAAGCTACAAACAATTGCCTTTAAATCTTTATC
AAATCCAAACTAAATTCAGAGATGAGATTCGCCCTCGTTTTGGTGTAATGCGTGCACGTGAATTTATAATGAAAGATGCT
TATTCATTTCATGAAAATAGTCAATGTCTACGCAATACTTATAATACAATGTACGCAACCTACTGCAATATCCTTGACAA
AATAGGCTTAGCTTATCGCCCTGTTAAAGCTGATACCGGAGCTATAGGTGGTGATAATAGTCATGAGTTTCAGGTATTAG
CAAATGCTGGTGAAGATATTATTTGCTATAGTAATGGTTCTGACTATGCTGCAAATATTGAGTTAGCAACTTATGCAAAA
CCTGATCTAAGCAAAAGAGTAAACTCTCAAAATACCATTGAGAAAATACACACTCCAAACATCAAAACTATCGAAAAACT
CTGTAAAGAGATGAGCTTTGATATTAAAAAGACTATCAAAACTATGGTTATCAAAGATGCCGGAGGAAATTTCTTTGCTT
TAGTTATCCGAGGTGATCATGAATTAAACGAAACTAAGATTAATAAGCTTGATCAAATTATTGCACCATATACATTAGCT
ACTAAAGAAGAGATTTTTTCAATATTTAATGCTAATCCAGGCTCGCTTGGTATATATAACTGCCCTATCAGTATAATCGC
TGACTACAGTGCAATTGCTATTACAGATCTAGTTTGTGGCGCTAATGAAGATGATTATCACTTTACAAATGTAAACTGGG
ATAGAGATGTAACTAACTATCAAATCGCCGACATTAGAAATGTAGTAACTGGCGATATTTCCCCTGATGGTAAAGGCACT
CTAGAACTTACAAATGGTATCGAGGTTGGTCATATTTTTGAACTTGAAGATGTCTACTCAAAACCAATGAATGCAAATAT
AATCGGTCAAGATGGTAAATCTAAACCTATGCTTATGGGTTGTTATGGTTTTGGAGTGTCGCGTGTGATGGCAGCAGCAA
TTGAGCAATCTCATGATGAAAATGGGATTATATGGCCTGAGTCTATTGCTCCATACCAAGTTGCTATACTACCTATCAAC
TACAATAAATCTGACAAGATTAAAGAAGTAGCAGATAAACTTTATCAAGACTTGCTTGGAGATGGTATTGATGTCCTATT
AGATGATCGAGGTGCTAGACCTGGCGTGATGTTCGCTGATGCTGATCTAATTGGTTACTCTCATCATGTAGTAATTGGCG
ATAGACTACTTGAGCAAGGGCTGATTGAATATAAGAATCGCAAAACTCAAGAAAAACAAGAGATAACTATTGCAGAGCTG
ATCAAGATTTTAAAATAA

Upstream 100 bases:

>100_bases
TCTCCTTAACTTCCTTCCATCTAGAGTCAATTTTTATATTTTATAAATTATTTTTTGCTATAATTTAGCTCATATTTTTA
TTTTTAGATTTATAGATCTT

Downstream 100 bases:

>100_bases
TCTCACCTATATAACTTTTTCTTTAAATTAATTTAGACATCAAATAAATACTTTTATTATCAACAATAAATCAACGAATA
AAGCTAAATTTATCTAACGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKATQTLIATTKELPKEAVLISHQYMLKAGLIKKLASGIYTWMPLGLKVLQKIQNIVRDEMNKAGASELLLPSILPSELL
QETHRWDKFGPELLKLHDRHNRDFCYGPTHEEPIVDMARDTIKSYKQLPLNLYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHENSQCLRNTYNTMYATYCNILDKIGLAYRPVKADTGAIGGDNSHEFQVLANAGEDIICYSNGSDYAANIELATYAK
PDLSKRVNSQNTIEKIHTPNIKTIEKLCKEMSFDIKKTIKTMVIKDAGGNFFALVIRGDHELNETKINKLDQIIAPYTLA
TKEEIFSIFNANPGSLGIYNCPISIIADYSAIAITDLVCGANEDDYHFTNVNWDRDVTNYQIADIRNVVTGDISPDGKGT
LELTNGIEVGHIFELEDVYSKPMNANIIGQDGKSKPMLMGCYGFGVSRVMAAAIEQSHDENGIIWPESIAPYQVAILPIN
YNKSDKIKEVADKLYQDLLGDGIDVLLDDRGARPGVMFADADLIGYSHHVVIGDRLLEQGLIEYKNRKTQEKQEITIAEL
IKILK

Sequences:

>Translated_565_residues
MKATQTLIATTKELPKEAVLISHQYMLKAGLIKKLASGIYTWMPLGLKVLQKIQNIVRDEMNKAGASELLLPSILPSELL
QETHRWDKFGPELLKLHDRHNRDFCYGPTHEEPIVDMARDTIKSYKQLPLNLYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHENSQCLRNTYNTMYATYCNILDKIGLAYRPVKADTGAIGGDNSHEFQVLANAGEDIICYSNGSDYAANIELATYAK
PDLSKRVNSQNTIEKIHTPNIKTIEKLCKEMSFDIKKTIKTMVIKDAGGNFFALVIRGDHELNETKINKLDQIIAPYTLA
TKEEIFSIFNANPGSLGIYNCPISIIADYSAIAITDLVCGANEDDYHFTNVNWDRDVTNYQIADIRNVVTGDISPDGKGT
LELTNGIEVGHIFELEDVYSKPMNANIIGQDGKSKPMLMGCYGFGVSRVMAAAIEQSHDENGIIWPESIAPYQVAILPIN
YNKSDKIKEVADKLYQDLLGDGIDVLLDDRGARPGVMFADADLIGYSHHVVIGDRLLEQGLIEYKNRKTQEKQEITIAEL
IKILK
>Mature_565_residues
MKATQTLIATTKELPKEAVLISHQYMLKAGLIKKLASGIYTWMPLGLKVLQKIQNIVRDEMNKAGASELLLPSILPSELL
QETHRWDKFGPELLKLHDRHNRDFCYGPTHEEPIVDMARDTIKSYKQLPLNLYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHENSQCLRNTYNTMYATYCNILDKIGLAYRPVKADTGAIGGDNSHEFQVLANAGEDIICYSNGSDYAANIELATYAK
PDLSKRVNSQNTIEKIHTPNIKTIEKLCKEMSFDIKKTIKTMVIKDAGGNFFALVIRGDHELNETKINKLDQIIAPYTLA
TKEEIFSIFNANPGSLGIYNCPISIIADYSAIAITDLVCGANEDDYHFTNVNWDRDVTNYQIADIRNVVTGDISPDGKGT
LELTNGIEVGHIFELEDVYSKPMNANIIGQDGKSKPMLMGCYGFGVSRVMAAAIEQSHDENGIIWPESIAPYQVAILPIN
YNKSDKIKEVADKLYQDLLGDGIDVLLDDRGARPGVMFADADLIGYSHHVVIGDRLLEQGLIEYKNRKTQEKQEITIAEL
IKILK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=241, Percent_Identity=41.0788381742739, Blast_Score=203, Evalue=4e-52,
Organism=Escherichia coli, GI1786392, Length=567, Percent_Identity=54.4973544973545, Blast_Score=671, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=246, Percent_Identity=33.739837398374, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI193203271, Length=107, Percent_Identity=29.9065420560748, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=545, Percent_Identity=29.3577981651376, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656200, Length=229, Percent_Identity=35.8078602620087, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63554; Mature: 63554

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATQTLIATTKELPKEAVLISHQYMLKAGLIKKLASGIYTWMPLGLKVLQKIQNIVRDE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGASELLLPSILPSELLQETHRWDKFGPELLKLHDRHNRDFCYGPTHEEPIVDMARD
HHHCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
TIKSYKQLPLNLYQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHENSQCLRNTYNTMYAT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YCNILDKIGLAYRPVKADTGAIGGDNSHEFQVLANAGEDIICYSNGSDYAANIELATYAK
HHHHHHHCCCCEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEEEEEEECCC
PDLSKRVNSQNTIEKIHTPNIKTIEKLCKEMSFDIKKTIKTMVIKDAGGNFFALVIRGDH
CCHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHEEEECCCCEEEEEEECCC
ELNETKINKLDQIIAPYTLATKEEIFSIFNANPGSLGIYNCPISIIADYSAIAITDLVCG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCHHHHHHHHHCC
ANEDDYHFTNVNWDRDVTNYQIADIRNVVTGDISPDGKGTLELTNGIEVGHIFELEDVYS
CCCCCCEEECCCCCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEHHHHHC
KPMNANIIGQDGKSKPMLMGCYGFGVSRVMAAAIEQSHDENGIIWPESIAPYQVAILPIN
CCCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEEC
YNKSDKIKEVADKLYQDLLGDGIDVLLDDRGARPGVMFADADLIGYSHHVVIGDRLLEQG
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEECCCCEEEEHHHHHHHH
LIEYKNRKTQEKQEITIAELIKILK
HHHHHCCCCHHHHHCCHHHHHHHHC
>Mature Secondary Structure
MKATQTLIATTKELPKEAVLISHQYMLKAGLIKKLASGIYTWMPLGLKVLQKIQNIVRDE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGASELLLPSILPSELLQETHRWDKFGPELLKLHDRHNRDFCYGPTHEEPIVDMARD
HHHCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
TIKSYKQLPLNLYQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHENSQCLRNTYNTMYAT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YCNILDKIGLAYRPVKADTGAIGGDNSHEFQVLANAGEDIICYSNGSDYAANIELATYAK
HHHHHHHCCCCEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEEEEEEECCC
PDLSKRVNSQNTIEKIHTPNIKTIEKLCKEMSFDIKKTIKTMVIKDAGGNFFALVIRGDH
CCHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHEEEECCCCEEEEEEECCC
ELNETKINKLDQIIAPYTLATKEEIFSIFNANPGSLGIYNCPISIIADYSAIAITDLVCG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCHHHHHHHHHCC
ANEDDYHFTNVNWDRDVTNYQIADIRNVVTGDISPDGKGTLELTNGIEVGHIFELEDVYS
CCCCCCEEECCCCCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEHHHHHC
KPMNANIIGQDGKSKPMLMGCYGFGVSRVMAAAIEQSHDENGIIWPESIAPYQVAILPIN
CCCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEEC
YNKSDKIKEVADKLYQDLLGDGIDVLLDDRGARPGVMFADADLIGYSHHVVIGDRLLEQG
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEECCCCEEEEHHHHHHHH
LIEYKNRKTQEKQEITIAELIKILK
HHHHHCCCCHHHHHCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA