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The map label for this gene is wbtF [H]

Identifier: 134301497

GI number: 134301497

Start: 413769

End: 414740

Strand: Direct

Name: wbtF [H]

Synonym: FTW_0416

Alternate gene names: 134301497

Gene position: 413769-414740 (Clockwise)

Preceding gene: 134301496

Following gene: 134301498

Centisome position: 21.79

GC content: 33.54

Gene sequence:

>972_bases
GTGGCTTACGATAATGTTAAATTTCCTCATGGTTCGTTTTTTTTGGTGACTGGAGGTGCAGGTTTTATTGGCTCTAATTT
ATGTGAAGTTTTACTTAGTAAGGGTTATAGAGTTAGGTGTTTAGATGATCTCTCAAATGGTCACTATCACAATGTTGAGC
CGTTTTTAACTAATTCTAATTATGAGTTTATAAAAGGTGATATTAGAGATTTAGATACTTGCATGAAAGCTTGTGAAGGT
ATTGATTATGTTCTACATCAAGCTGCTTGGGGAAGCGTACCAAGAAGTATTGAGATGCCATTAGTGTATGAAGATATAAA
TGTTAAAGGTACATTAAATATGCTTGAAGCGGCTAGACAAAATAACGTTAAAAAATTTGTCTATGCTTCTAGTTCATCAG
TATATGGTGATGAGCCAAATTTACCTAAAAAAGAAGGTAGAGAAGGAAATGTTTTATCACCCTATGCATTTACAAAGAAA
GCTAATGAAGAGTGGGCGAGACTATACACAAAGTTATATGGTCTAGATACTTATGGTCTAAGATATTTTAATGTTTTCGG
TAGAAGACAAGATCCTAATGGTGCGTATGCAGCAGTTATACCTAAATTTATCAAACAGTTATTAAATGATGAAGCGCCAA
CTATAAATGGAGATGGTAAACAGTCGAGAGATTTTACATATATAGAGAATGTTATTGAGGCAAATCTTAAAGCATGTTTA
GCAGATAGTAAGTATGCCGGAGAGTCTTTTAATATAGCTTATGGAGGTAGAGAGTATCTTATAGATTTGTACTATAATCT
TTGTGATGCCTTGGGTAAAAAAATAGAGCCAAATTTTGGTCCAGATAGAGCGGGTGATATTAAGCATAGTAATGCTGATA
TTTCGAAGGCTAGGAATATGCTCGGATATAATCCGGAATATGATTTTGAATTAGGCATAAAGCATGCTGTTGAGTGGTAT
TTAATTAATTAA

Upstream 100 bases:

>100_bases
ATAGGCTATATGCGCATAATTCTAGAAAGATTATCTTTGACATCAAAGGTAGTTTAGATAAATCTGAGTTTGAAAAAGAT
TATATTTATTGGAGATTGTA

Downstream 100 bases:

>100_bases
ATGGTATTTTAATCAAGTGTACATAAAAAAAGTGTCTTTTAAAATTTTATATTTATATTTACTAGCTTTTTGTATTATTT
TTAGTTTAGAATTTAAATTT

Product: NAD-dependent epimerase/dehydratase family protein

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAYDNVKFPHGSFFLVTGGAGFIGSNLCEVLLSKGYRVRCLDDLSNGHYHNVEPFLTNSNYEFIKGDIRDLDTCMKACEG
IDYVLHQAAWGSVPRSIEMPLVYEDINVKGTLNMLEAARQNNVKKFVYASSSSVYGDEPNLPKKEGREGNVLSPYAFTKK
ANEEWARLYTKLYGLDTYGLRYFNVFGRRQDPNGAYAAVIPKFIKQLLNDEAPTINGDGKQSRDFTYIENVIEANLKACL
ADSKYAGESFNIAYGGREYLIDLYYNLCDALGKKIEPNFGPDRAGDIKHSNADISKARNMLGYNPEYDFELGIKHAVEWY
LIN

Sequences:

>Translated_323_residues
MAYDNVKFPHGSFFLVTGGAGFIGSNLCEVLLSKGYRVRCLDDLSNGHYHNVEPFLTNSNYEFIKGDIRDLDTCMKACEG
IDYVLHQAAWGSVPRSIEMPLVYEDINVKGTLNMLEAARQNNVKKFVYASSSSVYGDEPNLPKKEGREGNVLSPYAFTKK
ANEEWARLYTKLYGLDTYGLRYFNVFGRRQDPNGAYAAVIPKFIKQLLNDEAPTINGDGKQSRDFTYIENVIEANLKACL
ADSKYAGESFNIAYGGREYLIDLYYNLCDALGKKIEPNFGPDRAGDIKHSNADISKARNMLGYNPEYDFELGIKHAVEWY
LIN
>Mature_322_residues
AYDNVKFPHGSFFLVTGGAGFIGSNLCEVLLSKGYRVRCLDDLSNGHYHNVEPFLTNSNYEFIKGDIRDLDTCMKACEGI
DYVLHQAAWGSVPRSIEMPLVYEDINVKGTLNMLEAARQNNVKKFVYASSSSVYGDEPNLPKKEGREGNVLSPYAFTKKA
NEEWARLYTKLYGLDTYGLRYFNVFGRRQDPNGAYAAVIPKFIKQLLNDEAPTINGDGKQSRDFTYIENVIEANLKACLA
DSKYAGESFNIAYGGREYLIDLYYNLCDALGKKIEPNFGPDRAGDIKHSNADISKARNMLGYNPEYDFELGIKHAVEWYL
IN

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=312, Percent_Identity=29.8076923076923, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI7657641, Length=319, Percent_Identity=32.6018808777429, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI56237023, Length=318, Percent_Identity=28.6163522012579, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI56118217, Length=318, Percent_Identity=28.6163522012579, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI189083684, Length=318, Percent_Identity=28.6163522012579, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI193211614, Length=250, Percent_Identity=28.4, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI8393516, Length=250, Percent_Identity=28.4, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786974, Length=314, Percent_Identity=32.8025477707006, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=30.791788856305, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI48994969, Length=338, Percent_Identity=29.2899408284024, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1790049, Length=227, Percent_Identity=28.6343612334802, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=270, Percent_Identity=22.962962962963, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788366, Length=157, Percent_Identity=31.8471337579618, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=316, Percent_Identity=29.746835443038, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17568069, Length=326, Percent_Identity=29.7546012269939, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=337, Percent_Identity=28.7833827893175, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=339, Percent_Identity=28.6135693215339, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI115532424, Length=321, Percent_Identity=29.2834890965732, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71987463, Length=130, Percent_Identity=35.3846153846154, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=336, Percent_Identity=31.547619047619, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19923002, Length=336, Percent_Identity=31.25, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356223, Length=312, Percent_Identity=28.525641025641, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 36408; Mature: 36277

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDNVKFPHGSFFLVTGGAGFIGSNLCEVLLSKGYRVRCLDDLSNGHYHNVEPFLTNSN
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEECCC
YEFIKGDIRDLDTCMKACEGIDYVLHQAAWGSVPRSIEMPLVYEDINVKGTLNMLEAARQ
CCEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
NNVKKFVYASSSSVYGDEPNLPKKEGREGNVLSPYAFTKKANEEWARLYTKLYGLDTYGL
CCCEEEEEECCCCCCCCCCCCCHHCCCCCCEECCHHHHHCCHHHHHHHHHHHHCCCHHHH
RYFNVFGRRQDPNGAYAAVIPKFIKQLLNDEAPTINGDGKQSRDFTYIENVIEANLKACL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ADSKYAGESFNIAYGGREYLIDLYYNLCDALGKKIEPNFGPDRAGDIKHSNADISKARNM
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LGYNPEYDFELGIKHAVEWYLIN
CCCCCCCCEECCHHHCEEEEEEC
>Mature Secondary Structure 
AYDNVKFPHGSFFLVTGGAGFIGSNLCEVLLSKGYRVRCLDDLSNGHYHNVEPFLTNSN
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEECCC
YEFIKGDIRDLDTCMKACEGIDYVLHQAAWGSVPRSIEMPLVYEDINVKGTLNMLEAARQ
CCEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
NNVKKFVYASSSSVYGDEPNLPKKEGREGNVLSPYAFTKKANEEWARLYTKLYGLDTYGL
CCCEEEEEECCCCCCCCCCCCCHHCCCCCCEECCHHHHHCCHHHHHHHHHHHHCCCHHHH
RYFNVFGRRQDPNGAYAAVIPKFIKQLLNDEAPTINGDGKQSRDFTYIENVIEANLKACL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ADSKYAGESFNIAYGGREYLIDLYYNLCDALGKKIEPNFGPDRAGDIKHSNADISKARNM
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LGYNPEYDFELGIKHAVEWYLIN
CCCCCCCCEECCHHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]