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The map label for this gene is bcr [H]

Identifier: 134301371

GI number: 134301371

Start: 263374

End: 264555

Strand: Reverse

Name: bcr [H]

Synonym: FTW_0255

Alternate gene names: 134301371

Gene position: 264555-263374 (Counterclockwise)

Preceding gene: 134301372

Following gene: 134301370

Centisome position: 13.94

GC content: 34.52

Gene sequence:

>1182_bases
ATGTATAAAGGTATCAGACCATCAATATTTATCTTAATTCTCATGGTCTCTTTGGGACCATTTGGTGATACCATTTATGC
ACCAGCATTGCCTGAGTTAAAAACAATTTTAGGTACTGACTATCCCCATGTGCAGCTTACTATAACCTCATATTTACTTG
GCTACTCGATTAGTCAGCTATTGTATGGACCATTCTCTGATAGATTTGGTCGCAAACCTGTAATGTTGGTAGGAACATGT
TTTTTTATAATTAGCTCTATAATATGTATATTTAGTAACCATATCAATACTTTGATATATGCTAGAATGATACAAGGCTT
TGGTGCAGCTGCTGGTGGAGTTATTGCTACAGTTGCTGTTAAAGATGCTTTCAAAGTTAATGAGCAAGGCGCAGTATTTG
CGATTATGAATATAGCCTTTGCTCTAGCTCCAGCATTTGGTGCAGTACTTGGAGTATTCCTTAGTCCTACAGCAATTTTT
TGGGTATTACTAGTTGCAGCTAGTATACTATTCATAAAAGTTGCGATCTTTTTCCCAGAAACAATTAGAGAAAAAAACTT
TGAAGCTTTAACTTTTAAATCTTTCTTTAAGAACTATTTTTTACTTTTTAAAGACCATCAGTTTTTCTTTGCCACTTTTG
TTTTGGGGATAAATATCAGTGTGATATACGCCTGTTTAGTCACAGCCCCAGATATATTTGTAAATATTCTCAAACTCGAG
AAATCTAACTTTTTATACTTATTAACAATAATGGTTACAGCTGTGGTACTAGGGTCTATTGTTTGCTCAAAACTAAGTCG
CCTAATAGCATATAAACATCTTGTAAATTTCGGCATGTTTTGTACTTTGATATCTGGAGTGTTATGCATCTATGCATTCA
AAAAACTTACAGGCTTAGAATTATCAATAGCCTTAACTGCAATATTATCACTAACATTTATAGGTGTCTCATTTAGTGTG
CCTTTACTAACACCTATTGCACTAGAAAACTTTACTACTACTGCAGGAGCTGCCTCGTCTGTGATGGGCTTTATGCAAAT
GGGCATAGCCTCAATAACAACCGCAATTATGAGTCAAATCAGCCTAGGCAGTGAATTTACGCTATCTTTTGCATTTGTAA
TTCTACCACTTATTGGTCTAACGATATTTTTACCTTATAGTTGCTATTTCCTCAAGAAATAA

Upstream 100 bases:

>100_bases
CTCCTTTAGCAACAAAAACTACACAAACAACAGAAACTAAATCTAAAGGCATCAATGCTACTATAATAATTGGTGATTTT
AGTAAAGGTAAGCCAAGCTA

Downstream 100 bases:

>100_bases
AGCTTAGTGCTCAGCTAAATTTAGGATTAAAATAATTATATGGTAAAATCTAGATTAACTAATATAATTTTATCGATCTT
AAATTATCAATGCAAAACTA

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQLLYGPFSDRFGRKPVMLVGTC
FFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAVKDAFKVNEQGAVFAIMNIAFALAPAFGAVLGVFLSPTAIF
WVLLVAASILFIKVAIFFPETIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLELSIALTAILSLTFIGVSFSV
PLLTPIALENFTTTAGAASSVMGFMQMGIASITTAIMSQISLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK

Sequences:

>Translated_393_residues
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQLLYGPFSDRFGRKPVMLVGTC
FFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAVKDAFKVNEQGAVFAIMNIAFALAPAFGAVLGVFLSPTAIF
WVLLVAASILFIKVAIFFPETIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLELSIALTAILSLTFIGVSFSV
PLLTPIALENFTTTAGAASSVMGFMQMGIASITTAIMSQISLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK
>Mature_393_residues
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQLLYGPFSDRFGRKPVMLVGTC
FFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAVKDAFKVNEQGAVFAIMNIAFALAPAFGAVLGVFLSPTAIF
WVLLVAASILFIKVAIFFPETIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLELSIALTAILSLTFIGVSFSV
PLLTPIALENFTTTAGAASSVMGFMQMGIASITTAIMSQISLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=366, Percent_Identity=27.8688524590164, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787065, Length=315, Percent_Identity=26.3492063492064, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082312, Length=353, Percent_Identity=26.628895184136, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI48994889, Length=203, Percent_Identity=32.0197044334975, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790146, Length=364, Percent_Identity=25, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790794, Length=221, Percent_Identity=24.4343891402715, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=166, Percent_Identity=30.7228915662651, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42966; Mature: 42966

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
LYGPFSDRFGRKPVMLVGTCFFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAV
HCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KDAFKVNEQGAVFAIMNIAFALAPAFGAVLGVFLSPTAIFWVLLVAASILFIKVAIFFPE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
HHHHCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LSIALTAILSLTFIGVSFSVPLLTPIALENFTTTAGAASSVMGFMQMGIASITTAIMSQI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MYKGIRPSIFILILMVSLGPFGDTIYAPALPELKTILGTDYPHVQLTITSYLLGYSISQL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
LYGPFSDRFGRKPVMLVGTCFFIISSIICIFSNHINTLIYARMIQGFGAAAGGVIATVAV
HCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KDAFKVNEQGAVFAIMNIAFALAPAFGAVLGVFLSPTAIFWVLLVAASILFIKVAIFFPE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TIREKNFEALTFKSFFKNYFLLFKDHQFFFATFVLGINISVIYACLVTAPDIFVNILKLE
HHHHCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KSNFLYLLTIMVTAVVLGSIVCSKLSRLIAYKHLVNFGMFCTLISGVLCIYAFKKLTGLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LSIALTAILSLTFIGVSFSVPLLTPIALENFTTTAGAASSVMGFMQMGIASITTAIMSQI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGSEFTLSFAFVILPLIGLTIFLPYSCYFLKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]