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The map label for this gene is parE [H]

Identifier: 134301370

GI number: 134301370

Start: 261401

End: 263284

Strand: Reverse

Name: parE [H]

Synonym: FTW_0254

Alternate gene names: 134301370

Gene position: 263284-261401 (Counterclockwise)

Preceding gene: 134301371

Following gene: 134301368

Centisome position: 13.87

GC content: 34.55

Gene sequence:

>1884_bases
ATGCAAAACTATAATGCTAAATCTATCGAAGTTCTTACTGGTTTAGACCCTGTCAAGAAAAGACCAGGAATGTATACTAA
TACAGAAAATCCAAATCATCTCATCCAAGAAATTATTGATAATAGCGTTGATGAAGTTCTTGCTGGATTTGCTAGCAAAA
TAAATATCACATTATACGAAGATAACAGTATCGAAGTAGCTGATGATGGTCGTGGTATGCCTGTAGATATTCATCCTGAG
CATAAAATGTCTGGTATTGAACTTATCATGACCAAACTTCATTCTGGCGGCAAATTTAGTAACAAAAATTATACTCATTC
TGGCGGTCTTCATGGAGTTGGGGTATCTGTTGTTAATGCTTTATCAACTAGACTTGAGGCAGAGATTAAAAGAGATGGTA
ATGTTTATCATATAGTCTTTGAGGATGGTTTTAAAACTAAAGATCTGGAAATTATTGATAATGTAGGCAAGAAAAATACT
GGAACAAAAATTAGATTTTGGCCAAATAAAAAATATTTTGATGATATCAAAGTCAATTTTAAAGCTCTTAAAAATCTGCT
TGAAGCAAAAGCGATACTTTGTAAGGCTCTCACAATAAAATATTCAAATGAGATAAAAAAAGAGAAGCTTACATGGCATT
TTGAAACTGGACTTAAAGGCTATCTTGACCATAAAATAGAAGCTGAAACACTCCCAGCAGAACCGTTTATGATCGATAAT
TTCTCAAATGGCGATTCTTATTTAGATGCAGTATTTTGCTGGTGTGAAGATCCTAGTGAAAGTATCAAGAATAGCTATGT
AAACCTAATTCCAACACCTCAAGATGGTACACATGTCACCGGACTTAAAAATGGAATTTACGATGCGATTAAGGCGTATA
TAGAGAAAAACTCCCTTAGTGTGAAGAATATCAAAATTACTGCAAATGACTCTTTTGCTCAACTTAACTATGTAATATCA
GTAAAAATTACAAATCCACAATTTGCTGGACAAACCAAAGAAAAACTTTCTAATAAAGATGTTACTAACTTTGTTGCTAC
TGCTGTTAAAGACTTACTCACCATATGGCTAAACCAAAACCCTGATGAGGCACGTCAAATTGTTGAAAATATTAGTAAAG
TTGCACAAAAAAGAATTAATGCAGACAAAAAAACCACACGTAAACGCATAATGAACACAACTATTCGTTTGCCTGGTAAA
CTTACAGATTGTATTAGTTCTGATGTTAATTCTACTGAGCTTTTCATCGTTGAGGGGGATTCTGCTGGAGGTTCTGCTAA
ACAAGCACGTGATAAAAACTTTCAAGCTGTTCTACCTCTTAAAGGTAAGATTCTTAATAGTTGGGAACTAGATGCTGATA
CAATCATGAACTCTCAAGAAATCCACAATATTGCTACAGCAATTGGTGTTGATCCTGATAGTGATGATATTTCTGCTCTA
AGATACAATAAAATATGTATCCTCGCCGATGCCGACTCTGATGGATTGCATATCGCAACACTACTATGTGCAATGTTTCT
AAAGCACTTTAGAAAACTTATTGAGAATGGTCATATTTATATAGCTCAACCTCCGCTGTTTAGAATTGATATTGGTAAAA
GCACTTTCTACGCTCTTGATGAAAATGAAAGAGATACTATCTTGACTAAAAACTCTAAATTACCAGGTAAGGTAAATATC
ATGCGCTTTAAGGGTCTAGGGGAGATGAATCCTGCACAATTACGTGAGTCAGCTATGGATGTATCATCAAGAAGACTTTT
ACAACTTACCATTTCTGATGTATATGATGATACTGAAATGTTAGACATGCTTTTGGCTAAAAAGAGAGCCAAAGATCGCC
GTGATTGGCTCGAGAACTATGGTGATAGAGCCAGTGTTGAATAA

Upstream 100 bases:

>100_bases
TCAAGAAATAAAGCTTAGTGCTCAGCTAAATTTAGGATTAAAATAATTATATGGTAAAATCTAGATTAACTAATATAATT
TTATCGATCTTAAATTATCA

Downstream 100 bases:

>100_bases
TAGACTCTTTTACTTACTTTTATTTTTTTATCAATTAATTAGAATTTCTACCAGATAACCTTATTCCATTCAAAACATTT
ACCAGGATCAGTTTTTCTCT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MQNYNAKSIEVLTGLDPVKKRPGMYTNTENPNHLIQEIIDNSVDEVLAGFASKINITLYEDNSIEVADDGRGMPVDIHPE
HKMSGIELIMTKLHSGGKFSNKNYTHSGGLHGVGVSVVNALSTRLEAEIKRDGNVYHIVFEDGFKTKDLEIIDNVGKKNT
GTKIRFWPNKKYFDDIKVNFKALKNLLEAKAILCKALTIKYSNEIKKEKLTWHFETGLKGYLDHKIEAETLPAEPFMIDN
FSNGDSYLDAVFCWCEDPSESIKNSYVNLIPTPQDGTHVTGLKNGIYDAIKAYIEKNSLSVKNIKITANDSFAQLNYVIS
VKITNPQFAGQTKEKLSNKDVTNFVATAVKDLLTIWLNQNPDEARQIVENISKVAQKRINADKKTTRKRIMNTTIRLPGK
LTDCISSDVNSTELFIVEGDSAGGSAKQARDKNFQAVLPLKGKILNSWELDADTIMNSQEIHNIATAIGVDPDSDDISAL
RYNKICILADADSDGLHIATLLCAMFLKHFRKLIENGHIYIAQPPLFRIDIGKSTFYALDENERDTILTKNSKLPGKVNI
MRFKGLGEMNPAQLRESAMDVSSRRLLQLTISDVYDDTEMLDMLLAKKRAKDRRDWLENYGDRASVE

Sequences:

>Translated_627_residues
MQNYNAKSIEVLTGLDPVKKRPGMYTNTENPNHLIQEIIDNSVDEVLAGFASKINITLYEDNSIEVADDGRGMPVDIHPE
HKMSGIELIMTKLHSGGKFSNKNYTHSGGLHGVGVSVVNALSTRLEAEIKRDGNVYHIVFEDGFKTKDLEIIDNVGKKNT
GTKIRFWPNKKYFDDIKVNFKALKNLLEAKAILCKALTIKYSNEIKKEKLTWHFETGLKGYLDHKIEAETLPAEPFMIDN
FSNGDSYLDAVFCWCEDPSESIKNSYVNLIPTPQDGTHVTGLKNGIYDAIKAYIEKNSLSVKNIKITANDSFAQLNYVIS
VKITNPQFAGQTKEKLSNKDVTNFVATAVKDLLTIWLNQNPDEARQIVENISKVAQKRINADKKTTRKRIMNTTIRLPGK
LTDCISSDVNSTELFIVEGDSAGGSAKQARDKNFQAVLPLKGKILNSWELDADTIMNSQEIHNIATAIGVDPDSDDISAL
RYNKICILADADSDGLHIATLLCAMFLKHFRKLIENGHIYIAQPPLFRIDIGKSTFYALDENERDTILTKNSKLPGKVNI
MRFKGLGEMNPAQLRESAMDVSSRRLLQLTISDVYDDTEMLDMLLAKKRAKDRRDWLENYGDRASVE
>Mature_627_residues
MQNYNAKSIEVLTGLDPVKKRPGMYTNTENPNHLIQEIIDNSVDEVLAGFASKINITLYEDNSIEVADDGRGMPVDIHPE
HKMSGIELIMTKLHSGGKFSNKNYTHSGGLHGVGVSVVNALSTRLEAEIKRDGNVYHIVFEDGFKTKDLEIIDNVGKKNT
GTKIRFWPNKKYFDDIKVNFKALKNLLEAKAILCKALTIKYSNEIKKEKLTWHFETGLKGYLDHKIEAETLPAEPFMIDN
FSNGDSYLDAVFCWCEDPSESIKNSYVNLIPTPQDGTHVTGLKNGIYDAIKAYIEKNSLSVKNIKITANDSFAQLNYVIS
VKITNPQFAGQTKEKLSNKDVTNFVATAVKDLLTIWLNQNPDEARQIVENISKVAQKRINADKKTTRKRIMNTTIRLPGK
LTDCISSDVNSTELFIVEGDSAGGSAKQARDKNFQAVLPLKGKILNSWELDADTIMNSQEIHNIATAIGVDPDSDDISAL
RYNKICILADADSDGLHIATLLCAMFLKHFRKLIENGHIYIAQPPLFRIDIGKSTFYALDENERDTILTKNSKLPGKVNI
MRFKGLGEMNPAQLRESAMDVSSRRLLQLTISDVYDDTEMLDMLLAKKRAKDRRDWLENYGDRASVE

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=586, Percent_Identity=24.4027303754266, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI19913406, Length=384, Percent_Identity=28.125, Blast_Score=105, Evalue=2e-22,
Organism=Escherichia coli, GI1789408, Length=629, Percent_Identity=54.3720190779014, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=38.3783783783784, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=633, Percent_Identity=25.5924170616114, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645845, Length=599, Percent_Identity=25.8764607679466, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=227, Percent_Identity=27.3127753303965, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=636, Percent_Identity=24.5283018867925, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=584, Percent_Identity=25, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 70346; Mature: 70346

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNYNAKSIEVLTGLDPVKKRPGMYTNTENPNHLIQEIIDNSVDEVLAGFASKINITLYE
CCCCCCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCEEEEEEEE
DNSIEVADDGRGMPVDIHPEHKMSGIELIMTKLHSGGKFSNKNYTHSGGLHGVGVSVVNA
CCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSTRLEAEIKRDGNVYHIVFEDGFKTKDLEIIDNVGKKNTGTKIRFWPNKKYFDDIKVNF
HHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCHHHEEEH
KALKNLLEAKAILCKALTIKYSNEIKKEKLTWHFETGLKGYLDHKIEAETLPAEPFMIDN
HHHHHHHHHHHHHHHHHEEECCCHHCHHHEEEEECCCCCHHHCCCCCCCCCCCCCEEEEC
FSNGDSYLDAVFCWCEDPSESIKNSYVNLIPTPQDGTHVTGLKNGIYDAIKAYIEKNSLS
CCCCCCEEEEEEEEECCHHHHHHCCCEEEEECCCCCCEECHHHHHHHHHHHHHHHHCCCE
VKNIKITANDSFAQLNYVISVKITNPQFAGQTKEKLSNKDVTNFVATAVKDLLTIWLNQN
EEEEEEEECCCEEEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
PDEARQIVENISKVAQKRINADKKTTRKRIMNTTIRLPGKLTDCISSDVNSTELFIVEGD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCHHHHHHHCCCCCEEEEEEECC
SAGGSAKQARDKNFQAVLPLKGKILNSWELDADTIMNSQEIHNIATAIGVDPDSDDISAL
CCCCCHHHHCCCCCEEEECCCCEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCEE
RYNKICILADADSDGLHIATLLCAMFLKHFRKLIENGHIYIAQPPLFRIDIGKSTFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCEEEEEC
ENERDTILTKNSKLPGKVNIMRFKGLGEMNPAQLRESAMDVSSRRLLQLTISDVYDDTEM
CCCCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHH
LDMLLAKKRAKDRRDWLENYGDRASVE
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQNYNAKSIEVLTGLDPVKKRPGMYTNTENPNHLIQEIIDNSVDEVLAGFASKINITLYE
CCCCCCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCEEEEEEEE
DNSIEVADDGRGMPVDIHPEHKMSGIELIMTKLHSGGKFSNKNYTHSGGLHGVGVSVVNA
CCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LSTRLEAEIKRDGNVYHIVFEDGFKTKDLEIIDNVGKKNTGTKIRFWPNKKYFDDIKVNF
HHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCHHHEEEH
KALKNLLEAKAILCKALTIKYSNEIKKEKLTWHFETGLKGYLDHKIEAETLPAEPFMIDN
HHHHHHHHHHHHHHHHHEEECCCHHCHHHEEEEECCCCCHHHCCCCCCCCCCCCCEEEEC
FSNGDSYLDAVFCWCEDPSESIKNSYVNLIPTPQDGTHVTGLKNGIYDAIKAYIEKNSLS
CCCCCCEEEEEEEEECCHHHHHHCCCEEEEECCCCCCEECHHHHHHHHHHHHHHHHCCCE
VKNIKITANDSFAQLNYVISVKITNPQFAGQTKEKLSNKDVTNFVATAVKDLLTIWLNQN
EEEEEEEECCCEEEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
PDEARQIVENISKVAQKRINADKKTTRKRIMNTTIRLPGKLTDCISSDVNSTELFIVEGD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCHHHHHHHCCCCCEEEEEEECC
SAGGSAKQARDKNFQAVLPLKGKILNSWELDADTIMNSQEIHNIATAIGVDPDSDDISAL
CCCCCHHHHCCCCCEEEECCCCEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCEE
RYNKICILADADSDGLHIATLLCAMFLKHFRKLIENGHIYIAQPPLFRIDIGKSTFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCEEEEEC
ENERDTILTKNSKLPGKVNIMRFKGLGEMNPAQLRESAMDVSSRRLLQLTISDVYDDTEM
CCCCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHH
LDMLLAKKRAKDRRDWLENYGDRASVE
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]