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The map label for this gene is pilT [H]

Identifier: 134301303

GI number: 134301303

Start: 176576

End: 177604

Strand: Direct

Name: pilT [H]

Synonym: FTW_0165

Alternate gene names: 134301303

Gene position: 176576-177604 (Clockwise)

Preceding gene: 134301302

Following gene: 134301305

Centisome position: 9.3

GC content: 36.05

Gene sequence:

>1029_bases
ATGATAAGAAAATTACTAACTCTTTGTGTGCAAAAAAAAGCCTCTGATCTACATTTATCCTCTGGTTGTAAGGCTAAATA
TAGAATAGATGGAGATTTGATTGATATTGAATCTTCACCTGTATTAAATGATAAGATGATTTCTCAGATGCTTTTAGAAA
TCATGACAGATGATCAAAAAGATGAGCTGATAGAAACCTACGAATGTGATTTTTCTATAGATGATAGAGATAATGATGCG
AGATTTAGGGTAAATGCTTTTTTCCATAACCGTGGTTATGGCGCGGTATTTCGTCGTTTAGAGAATACTATTCCAACGTT
AGATCAATTTGGAGCACCAAGAATTCTTAAAGAAGTTCAAGCTAAAAAAGGTGGCTTAATTCTAGTAACAGGACCCACAG
GTTCAGGTAAGAGTAGTACATTGGCAGCATTAGTCAATGAAATAAATCATAAGGAAGATTCTCATATTCTAACTATTGAG
GATCCCGTAGAATTTGTTCATACAAGTCAAAGATCACTAGTAAACCAACGTGAAGTTAAAAGAGATACAAAAAGCTTTAA
TGCGGCTCTTAAATCAGCACTAAGGGAGGATCCTGATTGTATTCTAGTTGGAGAGATGCGTGATTTAGAAACTATTAGAT
TAGCACTAGAGGCAGCTGAAACTGGGCATTTGGTTTTAGGGACATTACATACAATATCAGCTATAAAAACTGTTGATAGA
GTAATATCTGTATTTCCACCTGCTGAGCAGGAGCTTGTACGCAACATGCTTGCGGAATCATTACAAATCGTTATATCTCA
ACGTTTACTTAAACGTAAGGGCGGCGGGCGTGTCGCAGCATATGAAGTTCTTATTTCAAATACTGGTATTAGAAATATGA
TTAAAGAAAATAAACTTTCGCAAATTTACACCGCACTACAAACTGGAACAGCTAAGGGAATGGCGACAATCGAACAGAGC
ATTGAGAATCTTCTTAAGGCTGGAACAATTACACCAGAAGAAGCCGCTAAATACATTGTAGTAAGGTAA

Upstream 100 bases:

>100_bases
AAGTAATCAATCTGAAAAATTCTCTAATTGACTATTTTTTTTATTCTTTAATAGTGATAAAGTTCTAATACTATTATTAA
ATTTAAGTTCTATTCTTGAT

Downstream 100 bases:

>100_bases
TCATTTAAAAATCTAATATTTATATTGCTAGCAACAAATATTCAGTTCATATTTTATAGTTAGTCAAAATTTTAGTTCTT
CATGAAAGGTTTTTTGTAAT

Product: pilus retraction ATP ase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIRKLLTLCVQKKASDLHLSSGCKAKYRIDGDLIDIESSPVLNDKMISQMLLEIMTDDQKDELIETYECDFSIDDRDNDA
RFRVNAFFHNRGYGAVFRRLENTIPTLDQFGAPRILKEVQAKKGGLILVTGPTGSGKSSTLAALVNEINHKEDSHILTIE
DPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDCILVGEMRDLETIRLALEAAETGHLVLGTLHTISAIKTVDR
VISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLSQIYTALQTGTAKGMATIEQS
IENLLKAGTITPEEAAKYIVVR

Sequences:

>Translated_342_residues
MIRKLLTLCVQKKASDLHLSSGCKAKYRIDGDLIDIESSPVLNDKMISQMLLEIMTDDQKDELIETYECDFSIDDRDNDA
RFRVNAFFHNRGYGAVFRRLENTIPTLDQFGAPRILKEVQAKKGGLILVTGPTGSGKSSTLAALVNEINHKEDSHILTIE
DPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDCILVGEMRDLETIRLALEAAETGHLVLGTLHTISAIKTVDR
VISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLSQIYTALQTGTAKGMATIEQS
IENLLKAGTITPEEAAKYIVVR
>Mature_342_residues
MIRKLLTLCVQKKASDLHLSSGCKAKYRIDGDLIDIESSPVLNDKMISQMLLEIMTDDQKDELIETYECDFSIDDRDNDA
RFRVNAFFHNRGYGAVFRRLENTIPTLDQFGAPRILKEVQAKKGGLILVTGPTGSGKSSTLAALVNEINHKEDSHILTIE
DPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDCILVGEMRDLETIRLALEAAETGHLVLGTLHTISAIKTVDR
VISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLSQIYTALQTGTAKGMATIEQS
IENLLKAGTITPEEAAKYIVVR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=45.2887537993921, Blast_Score=284, Evalue=8e-78,
Organism=Escherichia coli, GI1786296, Length=283, Percent_Identity=31.8021201413428, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789723, Length=283, Percent_Identity=34.6289752650177, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38032; Mature: 38032

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKLLTLCVQKKASDLHLSSGCKAKYRIDGDLIDIESSPVLNDKMISQMLLEIMTDDQK
CHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHCCCCH
DELIETYECDFSIDDRDNDARFRVNAFFHNRGYGAVFRRLENTIPTLDQFGAPRILKEVQ
HHHHHHHCCCEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCHHHCCCHHHHHHHH
AKKGGLILVTGPTGSGKSSTLAALVNEINHKEDSHILTIEDPVEFVHTSQRSLVNQREVK
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
RDTKSFNAALKSALREDPDCILVGEMRDLETIRLALEAAETGHLVLGTLHTISAIKTVDR
HHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
VISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCHHHHHHHHHHH
QIYTALQTGTAKGMATIEQSIENLLKAGTITPEEAAKYIVVR
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEC
>Mature Secondary Structure
MIRKLLTLCVQKKASDLHLSSGCKAKYRIDGDLIDIESSPVLNDKMISQMLLEIMTDDQK
CHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHCCCCH
DELIETYECDFSIDDRDNDARFRVNAFFHNRGYGAVFRRLENTIPTLDQFGAPRILKEVQ
HHHHHHHCCCEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCHHHCCCHHHHHHHH
AKKGGLILVTGPTGSGKSSTLAALVNEINHKEDSHILTIEDPVEFVHTSQRSLVNQREVK
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
RDTKSFNAALKSALREDPDCILVGEMRDLETIRLALEAAETGHLVLGTLHTISAIKTVDR
HHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
VISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCHHHHHHHHHHH
QIYTALQTGTAKGMATIEQSIENLLKAGTITPEEAAKYIVVR
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]