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The map label for this gene is sucB [H]

Identifier: 134301291

GI number: 134301291

Start: 164927

End: 166396

Strand: Direct

Name: sucB [H]

Synonym: FTW_0153

Alternate gene names: 134301291

Gene position: 164927-166396 (Clockwise)

Preceding gene: 134301290

Following gene: 134301292

Centisome position: 8.69

GC content: 37.96

Gene sequence:

>1470_bases
ATGGTTGAATTAAAAGTACCTATGTTCCCAGAGTCTGTAGCAGATGGCACATTAGCTCAATGGAATAAAAACGAAGGTGA
CTTTGTAAATGAGGGCGATATCTTGGCAGAGATTGAGACTGATAAAGTTGTTCTAGAAGTACCTGCAACATCTAGTGGTG
TTTTAAAAGGGATAAAAAAACATGCTGGTGATACAGTGCTTTCAGAAGAGTCATTAGCGATCATTGATACTGCTGTTTCT
ACATCTGAACCTAACCAACAAACTACTAATCAAGGAAATGCTTCAGAAGCAACTGCTACTGGGCAAGAAATTGATATTAA
GGCGCCTGTATTTCCAGAGTCTGTAGCAGATGGCACGATCTCAGAGTGGCATAAGAAAGAGGGTGAGGCTGTTTCTGAGG
GTGATATCTTAGCAGAGATTGAGACTGATAAGGTTGTTCTAGAGGTTCCGGCAACATCAAATGGTGTTTTGACAAAAATA
TTAAAAACAGCAGGAGAGACTGTACTATCTGCAGAGCTTATCGCTAAGATTACAGCAGGAGGCGCAACTGCTACTACGAA
ATCAGAAGCTTCGGTGGGAGTTTCTCAAGCAAATAATGATCCGCATCTAGTACCTTCAGCACGTAAAGCTTTTAATGCAA
GCGGCTTGGATACTGCTGCTAATATCGAAGGTACAGGTAAAAAAGGGCGTATAACTTCTGAAGATGTCAAAAAAGCAGTT
GCATCAGTAAATAAACCTCAACAACAGACAGTTGTTATAAATCAAGGTGCTAGATATGAAAAAAGAGTCAAGATGACTCG
TCTGCGTCAGACTATAGCAAATAGGTTAGTTGAGGTTCAACATACTAATGCAATCTTAACTACTTTCAATGAAGTAGATA
TGAGTGCAGTTATGGAGCTTAGAAACAAATATAAAGATATGTTTGTCAAAGAACATGATACTAAGCTTGGCTTTATGTCT
TTCTTTATCAAAGCAGCAACAGAAGCACTTAAGAAATTCCCAGATGTAAATGCCTCTATTGATGGTGATGAGATTGTTTA
CCATAATTATTTTGATATTGGTATTGCTGTAGGTACTGATAGGGGTCTAGTGGTACCTGTACTAAGAGATACAGATACTA
AATCTCTAGCTGAATTAGAAGCCGATGTTTTAGACAAAGCGATTAAAGGTCGTGATGGTAAATTAAGCCTTGAAGATATG
CAAGGTGGTACATTTACGATTACAAATGGCGGAACTTATGGTTCGATGTTATCTACGCCTATTATTAATTCACCGCAAAG
TGCTATTTTAGGTATGCATAATATTGTTGAGCGTCCTGTAGTTGTTAAGGGTGAGATTAAGATTCGTCCAATTATGTATT
TAGCGTTATCTTACGACCATAGAATCATTGATGGCGGTACATCTGTAAGATTCTTGAAGATGATCAAAGAGCTAATTGAA
GATCCAAATAGAATTCTTCTACAAGTATAG

Upstream 100 bases:

>100_bases
ACACCTGCTGTTGGATATCATGCCTTATATGTAAAACAGCAGGAAGAAATTATTAATACAGCTTTAGAAATATAAATTAA
ATTATTACTAGGAGTAAAAG

Downstream 100 bases:

>100_bases
TTATTATTCTTTTTTAAATTATCCTAAAGGTACTTTTTTATGAACTTAGATTTTATTAAGAGTAAGATTGCAGCTGTGCC
TGATTTTCCAAAACCAGGAA

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MVELKVPMFPESVADGTLAQWNKNEGDFVNEGDILAEIETDKVVLEVPATSSGVLKGIKKHAGDTVLSEESLAIIDTAVS
TSEPNQQTTNQGNASEATATGQEIDIKAPVFPESVADGTISEWHKKEGEAVSEGDILAEIETDKVVLEVPATSNGVLTKI
LKTAGETVLSAELIAKITAGGATATTKSEASVGVSQANNDPHLVPSARKAFNASGLDTAANIEGTGKKGRITSEDVKKAV
ASVNKPQQQTVVINQGARYEKRVKMTRLRQTIANRLVEVQHTNAILTTFNEVDMSAVMELRNKYKDMFVKEHDTKLGFMS
FFIKAATEALKKFPDVNASIDGDEIVYHNYFDIGIAVGTDRGLVVPVLRDTDTKSLAELEADVLDKAIKGRDGKLSLEDM
QGGTFTITNGGTYGSMLSTPIINSPQSAILGMHNIVERPVVVKGEIKIRPIMYLALSYDHRIIDGGTSVRFLKMIKELIE
DPNRILLQV

Sequences:

>Translated_489_residues
MVELKVPMFPESVADGTLAQWNKNEGDFVNEGDILAEIETDKVVLEVPATSSGVLKGIKKHAGDTVLSEESLAIIDTAVS
TSEPNQQTTNQGNASEATATGQEIDIKAPVFPESVADGTISEWHKKEGEAVSEGDILAEIETDKVVLEVPATSNGVLTKI
LKTAGETVLSAELIAKITAGGATATTKSEASVGVSQANNDPHLVPSARKAFNASGLDTAANIEGTGKKGRITSEDVKKAV
ASVNKPQQQTVVINQGARYEKRVKMTRLRQTIANRLVEVQHTNAILTTFNEVDMSAVMELRNKYKDMFVKEHDTKLGFMS
FFIKAATEALKKFPDVNASIDGDEIVYHNYFDIGIAVGTDRGLVVPVLRDTDTKSLAELEADVLDKAIKGRDGKLSLEDM
QGGTFTITNGGTYGSMLSTPIINSPQSAILGMHNIVERPVVVKGEIKIRPIMYLALSYDHRIIDGGTSVRFLKMIKELIE
DPNRILLQV
>Mature_489_residues
MVELKVPMFPESVADGTLAQWNKNEGDFVNEGDILAEIETDKVVLEVPATSSGVLKGIKKHAGDTVLSEESLAIIDTAVS
TSEPNQQTTNQGNASEATATGQEIDIKAPVFPESVADGTISEWHKKEGEAVSEGDILAEIETDKVVLEVPATSNGVLTKI
LKTAGETVLSAELIAKITAGGATATTKSEASVGVSQANNDPHLVPSARKAFNASGLDTAANIEGTGKKGRITSEDVKKAV
ASVNKPQQQTVVINQGARYEKRVKMTRLRQTIANRLVEVQHTNAILTTFNEVDMSAVMELRNKYKDMFVKEHDTKLGFMS
FFIKAATEALKKFPDVNASIDGDEIVYHNYFDIGIAVGTDRGLVVPVLRDTDTKSLAELEADVLDKAIKGRDGKLSLEDM
QGGTFTITNGGTYGSMLSTPIINSPQSAILGMHNIVERPVVVKGEIKIRPIMYLALSYDHRIIDGGTSVRFLKMIKELIE
DPNRILLQV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=237, Percent_Identity=53.5864978902954, Blast_Score=275, Evalue=8e-74,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=30.2173913043478, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=30.4347826086957, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=27.1461716937355, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI31711992, Length=292, Percent_Identity=30.4794520547945, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.6459627329193, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786946, Length=404, Percent_Identity=51.980198019802, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1786305, Length=510, Percent_Identity=28.6274509803922, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=406, Percent_Identity=41.6256157635468, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=28.6046511627907, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=25.8992805755396, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=236, Percent_Identity=30.9322033898305, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=392, Percent_Identity=45.6632653061224, Blast_Score=332, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=26, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=56.8281938325991, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=28.0285035629454, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=31.9148936170213, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=31.9148936170213, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 52749; Mature: 52749

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELKVPMFPESVADGTLAQWNKNEGDFVNEGDILAEIETDKVVLEVPATSSGVLKGIKK
CEEEECCCCCHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHH
HAGDTVLSEESLAIIDTAVSTSEPNQQTTNQGNASEATATGQEIDIKAPVFPESVADGTI
HCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCH
SEWHKKEGEAVSEGDILAEIETDKVVLEVPATSNGVLTKILKTAGETVLSAELIAKITAG
HHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCC
GATATTKSEASVGVSQANNDPHLVPSARKAFNASGLDTAANIEGTGKKGRITSEDVKKAV
CCCCCCCCHHHCCHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ASVNKPQQQTVVINQGARYEKRVKMTRLRQTIANRLVEVQHTNAILTTFNEVDMSAVMEL
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEHCCCCEEEEEHHHHHHHHHHHH
RNKYKDMFVKEHDTKLGFMSFFIKAATEALKKFPDVNASIDGDEIVYHNYFDIGIAVGTD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECC
RGLVVPVLRDTDTKSLAELEADVLDKAIKGRDGKLSLEDMQGGTFTITNGGTYGSMLSTP
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHEECCCCEEEEECCCCCCHHHCCC
IINSPQSAILGMHNIVERPVVVKGEIKIRPIMYLALSYDHRIIDGGTSVRFLKMIKELIE
CCCCCHHHHHHHHHHHCCCEEEECCEEEEEEEEEEEECCCEEECCCCHHHHHHHHHHHHC
DPNRILLQV
CCCEEEEEC
>Mature Secondary Structure
MVELKVPMFPESVADGTLAQWNKNEGDFVNEGDILAEIETDKVVLEVPATSSGVLKGIKK
CEEEECCCCCHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHH
HAGDTVLSEESLAIIDTAVSTSEPNQQTTNQGNASEATATGQEIDIKAPVFPESVADGTI
HCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCH
SEWHKKEGEAVSEGDILAEIETDKVVLEVPATSNGVLTKILKTAGETVLSAELIAKITAG
HHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCC
GATATTKSEASVGVSQANNDPHLVPSARKAFNASGLDTAANIEGTGKKGRITSEDVKKAV
CCCCCCCCHHHCCHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ASVNKPQQQTVVINQGARYEKRVKMTRLRQTIANRLVEVQHTNAILTTFNEVDMSAVMEL
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEHCCCCEEEEEHHHHHHHHHHHH
RNKYKDMFVKEHDTKLGFMSFFIKAATEALKKFPDVNASIDGDEIVYHNYFDIGIAVGTD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECC
RGLVVPVLRDTDTKSLAELEADVLDKAIKGRDGKLSLEDMQGGTFTITNGGTYGSMLSTP
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHEECCCCEEEEECCCCCCHHHCCC
IINSPQSAILGMHNIVERPVVVKGEIKIRPIMYLALSYDHRIIDGGTSVRFLKMIKELIE
CCCCCHHHHHHHHHHHCCCEEEECCEEEEEEEEEEEECCCEEECCCCHHHHHHHHHHHHC
DPNRILLQV
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]