Click here to switch to the map view.

The map label for this gene is rph

Identifier: 134300519

GI number: 134300519

Start: 2908790

End: 2909527

Strand: Reverse

Name: rph

Synonym: Dred_2684

Alternate gene names: 134300519

Gene position: 2909527-2908790 (Counterclockwise)

Preceding gene: 134300520

Following gene: 134300518

Centisome position: 80.64

GC content: 48.37

Gene sequence:

>738_bases
TTGGACAGAGTTGACGGAAGAAAGCAGGGACAAATACGTCCAGTGCGCATGACACCTAATTATAACAAATACGCTGAGGG
TTCTGTTTTGATTGAAGTAGGAGATACGCGGGTTATTTGTACTGCCACCGTGGAAGAAAGGGTACCGCCCTTCTTGAAGG
GATTAGGCAAAGGCTGGGTAACTGCCGAATATTCTATGCTTCCCCGGGCCACCGGTAGCCGTACCATCCGTGAAGCAGCC
AGAGGAAAACTTGGTGGTAGAACAATGGAGATTCAAAGACTGATTGGTCGTGCCCTTCGTTCAGTGGTGAATCTGGAAGC
CTTGGGTGAACGTAGCCTAACAATGGATTGTGATGTTATTCAGGCCGATGGGGGTACCCGTACGGCATCCATTACCGGAG
CCTATGTGGCAATGATTTTTGCCTTGAACAGATTGGTTGAACAGGGTATGCTAGAGAAAATACCCGTCAGTGACTTTATA
GCTGCTACCAGTGTAGGGATTGTCAAGGGCAGACCGGTTATTGACCTGTGCTACGCAGAGGACTCTGTTGCTGAAGTGGA
TATGAACGTGGTTATGACTGGAACAGGGCGATTTGTTGAAATTCAGGGTACCGGGGAGGAAGCCACCTTCTCCAGAGAAG
AAATGAATCAACTTCTGGATTTAGCCGCAGCGGGTATTGAAGAATTAAATAAGGTTCAGCGCCAGGTACTGGGAGATATA
GCAGCTAAGATAGGGTAG

Upstream 100 bases:

>100_bases
GTGGTAGAAGGAGACAGCCTCGTAACCCCACCGGATCAGTTCCAGCTTATGCGGAGGGCACGCAGGCGTTCCAAACAGAT
TACTTAAGGAGGCAATCCAT

Downstream 100 bases:

>100_bases
GTGTAATTTTTGAAGTTAGTTTTAGCAACCAACAATAAAGGCAAAGTGAAAGAGTTGGCCGAGCTATTAAAACCCTGCGG
GTATCAGGTAGTTTCCATCG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MDRVDGRKQGQIRPVRMTPNYNKYAEGSVLIEVGDTRVICTATVEERVPPFLKGLGKGWVTAEYSMLPRATGSRTIREAA
RGKLGGRTMEIQRLIGRALRSVVNLEALGERSLTMDCDVIQADGGTRTASITGAYVAMIFALNRLVEQGMLEKIPVSDFI
AATSVGIVKGRPVIDLCYAEDSVAEVDMNVVMTGTGRFVEIQGTGEEATFSREEMNQLLDLAAAGIEELNKVQRQVLGDI
AAKIG

Sequences:

>Translated_245_residues
MDRVDGRKQGQIRPVRMTPNYNKYAEGSVLIEVGDTRVICTATVEERVPPFLKGLGKGWVTAEYSMLPRATGSRTIREAA
RGKLGGRTMEIQRLIGRALRSVVNLEALGERSLTMDCDVIQADGGTRTASITGAYVAMIFALNRLVEQGMLEKIPVSDFI
AATSVGIVKGRPVIDLCYAEDSVAEVDMNVVMTGTGRFVEIQGTGEEATFSREEMNQLLDLAAAGIEELNKVQRQVLGDI
AAKIG
>Mature_245_residues
MDRVDGRKQGQIRPVRMTPNYNKYAEGSVLIEVGDTRVICTATVEERVPPFLKGLGKGWVTAEYSMLPRATGSRTIREAA
RGKLGGRTMEIQRLIGRALRSVVNLEALGERSLTMDCDVIQADGGTRTASITGAYVAMIFALNRLVEQGMLEKIPVSDFI
AATSVGIVKGRPVIDLCYAEDSVAEVDMNVVMTGTGRFVEIQGTGEEATFSREEMNQLLDLAAAGIEELNKVQRQVLGDI
AAKIG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=63.8095238095238, Blast_Score=276, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI71981632, Length=180, Percent_Identity=29.4444444444444, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_DESRM (A4J7Y7)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001114015.1
- ProteinModelPortal:   A4J7Y7
- SMR:   A4J7Y7
- STRING:   A4J7Y7
- GeneID:   4955983
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2684
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   A4J7Y7
- ProtClustDB:   PRK00173
- BioCyc:   DRED349161:DRED_2684-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26567; Mature: 26567

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVDGRKQGQIRPVRMTPNYNKYAEGSVLIEVGDTRVICTATVEERVPPFLKGLGKGWV
CCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEEECHHHHCCHHHHHCCCCCE
TAEYSMLPRATGSRTIREAARGKLGGRTMEIQRLIGRALRSVVNLEALGERSLTMDCDVI
EEEHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEE
QADGGTRTASITGAYVAMIFALNRLVEQGMLEKIPVSDFIAATSVGIVKGRPVIDLCYAE
ECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCEEEEEECC
DSVAEVDMNVVMTGTGRFVEIQGTGEEATFSREEMNQLLDLAAAGIEELNKVQRQVLGDI
CCHHEECEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKIG
HHHCC
>Mature Secondary Structure
MDRVDGRKQGQIRPVRMTPNYNKYAEGSVLIEVGDTRVICTATVEERVPPFLKGLGKGWV
CCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEEECHHHHCCHHHHHCCCCCE
TAEYSMLPRATGSRTIREAARGKLGGRTMEIQRLIGRALRSVVNLEALGERSLTMDCDVI
EEEHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEE
QADGGTRTASITGAYVAMIFALNRLVEQGMLEKIPVSDFIAATSVGIVKGRPVIDLCYAE
ECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCCEEEEEECC
DSVAEVDMNVVMTGTGRFVEIQGTGEEATFSREEMNQLLDLAAAGIEELNKVQRQVLGDI
CCHHEECEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKIG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA