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The map label for this gene is yjbJ [H]

Identifier: 134300350

GI number: 134300350

Start: 2731814

End: 2732467

Strand: Reverse

Name: yjbJ [H]

Synonym: Dred_2511

Alternate gene names: 134300350

Gene position: 2732467-2731814 (Counterclockwise)

Preceding gene: 134300351

Following gene: 134300349

Centisome position: 75.73

GC content: 47.71

Gene sequence:

>654_bases
ATGGATGCAAGTTTAGTGGCACAATTGATAAGGCTACAGGCCTTGGAAATTGGTATAAATGGTACACAGGAAAATAAAAG
TGAACAATCAGCGGAATTTTCTCTGATGTTGGCTAAGCTTATTGCTGCTAAGGCTGGAGTAAGGGGGAATACCTTGCCTT
TAAATCCCGGCGTGAATCTGGCAGAGGTGCCTTTCCTGCCTAGTAAACAAGCTAGGGCTTCTGCTGCCGCAGCAGCAGCT
TCTTTTCGTGCTGCAGTTGGACCTGTGCGGGAATATGAGGCTATGGTGGAAAAATCAGCTTTACGTCATGGCATAGATCC
TGCCCTTTGTAAGGCTGTGGCCCGGGCGGAATCTGACTTTAATCCCAGGGTAACTTCCCGGACCGGGGCCATGGGTTTAA
TGCAACTGATGCCTGGCACGGCCCGCGATCTAGGAGTAAAAAATCCCTATGATCCAGAACAAAATGCTGATGGTGGCGTC
CGCTATTTAAAATCCATGCTGGAACGCTTCGATGGTGATGTAAACAAGGCCTTAGCGGCCTATAATGCTGGTCCTGGGGC
TGTTGAACGTTATGGAGGGATACCTCCCTATGAGGAAACTACTCGATATATACAGAAAGTAATTCGTTACCAGCAAAAGT
ATACTGGGGTTTAA

Upstream 100 bases:

>100_bases
AAGAATGTTTCAGGTATCGGTGATAAAAAGTTTGAGGATTTACAGCACCGTATAACCTTACAATAGCAAACCATAAAGAA
TTGGTACAGGATGTGAACAA

Downstream 100 bases:

>100_bases
TATATGCAGCGCCCCCTGGTTTTTATGATACTGGCCTTTATGCTGGGTATTATGGTAACCTCTGTCCTTAAAGTTAATAT
ACTCGTTGCGATGGGAGCAG

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MDASLVAQLIRLQALEIGINGTQENKSEQSAEFSLMLAKLIAAKAGVRGNTLPLNPGVNLAEVPFLPSKQARASAAAAAA
SFRAAVGPVREYEAMVEKSALRHGIDPALCKAVARAESDFNPRVTSRTGAMGLMQLMPGTARDLGVKNPYDPEQNADGGV
RYLKSMLERFDGDVNKALAAYNAGPGAVERYGGIPPYEETTRYIQKVIRYQQKYTGV

Sequences:

>Translated_217_residues
MDASLVAQLIRLQALEIGINGTQENKSEQSAEFSLMLAKLIAAKAGVRGNTLPLNPGVNLAEVPFLPSKQARASAAAAAA
SFRAAVGPVREYEAMVEKSALRHGIDPALCKAVARAESDFNPRVTSRTGAMGLMQLMPGTARDLGVKNPYDPEQNADGGV
RYLKSMLERFDGDVNKALAAYNAGPGAVERYGGIPPYEETTRYIQKVIRYQQKYTGV
>Mature_217_residues
MDASLVAQLIRLQALEIGINGTQENKSEQSAEFSLMLAKLIAAKAGVRGNTLPLNPGVNLAEVPFLPSKQARASAAAAAA
SFRAAVGPVREYEAMVEKSALRHGIDPALCKAVARAESDFNPRVTSRTGAMGLMQLMPGTARDLGVKNPYDPEQNADGGV
RYLKSMLERFDGDVNKALAAYNAGPGAVERYGGIPPYEETTRYIQKVIRYQQKYTGV

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=126, Percent_Identity=37.3015873015873, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI171474010, Length=109, Percent_Identity=37.6146788990826, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082191, Length=119, Percent_Identity=31.9327731092437, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23286; Mature: 23286

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASLVAQLIRLQALEIGINGTQENKSEQSAEFSLMLAKLIAAKAGVRGNTLPLNPGVNL
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
AEVPFLPSKQARASAAAAAASFRAAVGPVREYEAMVEKSALRHGIDPALCKAVARAESDF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
NPRVTSRTGAMGLMQLMPGTARDLGVKNPYDPEQNADGGVRYLKSMLERFDGDVNKALAA
CCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
YNAGPGAVERYGGIPPYEETTRYIQKVIRYQQKYTGV
HCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDASLVAQLIRLQALEIGINGTQENKSEQSAEFSLMLAKLIAAKAGVRGNTLPLNPGVNL
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
AEVPFLPSKQARASAAAAAASFRAAVGPVREYEAMVEKSALRHGIDPALCKAVARAESDF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
NPRVTSRTGAMGLMQLMPGTARDLGVKNPYDPEQNADGGVRYLKSMLERFDGDVNKALAA
CCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
YNAGPGAVERYGGIPPYEETTRYIQKVIRYQQKYTGV
HCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]