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The map label for this gene is dppA [H]

Identifier: 134300293

GI number: 134300293

Start: 2669074

End: 2670660

Strand: Reverse

Name: dppA [H]

Synonym: Dred_2454

Alternate gene names: 134300293

Gene position: 2670660-2669074 (Counterclockwise)

Preceding gene: 134300297

Following gene: 134300292

Centisome position: 74.02

GC content: 47.01

Gene sequence:

>1587_bases
ATGTTCCGTAAGAAAAGTTTTTTCATGCTGTTGCTGGTATCTTTGCTGGCAGTGGCCATGGTTGGCTGTGGCGGCGGTGA
CAACAAAGAAAAGGCTGCTGACAAAGCCAAGCAAAAGGTATTTGTTTTTGGCCAGGGTTCCGATCCCAGGGGCTTGGATC
CGGCTTTTGTTGATGATGGTGAATCTGCCAACCCTATTGTCAATATTTATGACGGTCTTGTTCGTTATAAAACTGGCAGC
ACTGAGATTGAACCTGCCTTGGCTACCGAGTGGTCTTCCTCAGAGGATGGCAAAGAATGGACCTTTAAGTTACGTCAAGG
CGTAAAATTCCACGACGGAACCCCCTTTAACGCCGATGCGGTGGTATTCAGCGTAAACCGTCAATTGCCTCCTAATCGAA
CCGATGCTATGCCCTATGCTTCCTTTACCTTTGGTCCTGTGGAAAAGATTGAGAAAGTGGACGACTACACAGTTAAGTTT
ATCCTCAACGAGCCCTATGCTCCCTTCTTGGCTAACCTGGCAATGGCTCTGGCTGCCCCCATCGTTAGCCCTGCAGCCGT
TGAAAAATATGGTGACAAATTTATTGAGAACCCTGTTGGTACCGGACCCTTCAAGTTTGTTGAGTGGAAAAAGGGTCAGC
AAATCGTGTTGGAAGCCAACAAGGAATACTGGGGTGGCGCTCCCAAACTTGACAAACTGGTTTACAAGTTTATTAAAGAA
AACTCTGTACGTGCCAGTGAGTTAAGAACCGGGTCCATTCAGGCCATGAACGGCGTGGACCCTAACGATGTGAAGATGTT
GGAAGAGGCAGGTCTGAATGTAATCAAAAACCCCGGTATGAATATCAACTACCTGGCGTTTTTCTGCAACAAGAAGCCCT
TTGACAATCCTAAACTACGTTTAGCTGTTGCTCATGCCATTAACCGTGAGAACTTAATCAACTTCCTGTACCAGGGTCTG
GCTGAGCTTCCTAATAGTATGCTGCCCAACTTTATGCCCGGACATGATAAGTCCCTGCAGGCTCCTGAGTATAACCCTGA
AAAGGCCAAGCAACTGCTGGCCGAAGCAGGTTATCCCAATGGCTTGAAGGTTACCCTGTTAACCTACTCCACCGTGCGTC
CTTATAACCCCGTGGGTGGAGATAAACTGGCTGCTGCTGTACAAGCTGATCTACGTAAAGTAGGGATCGAAGCAGAAATT
AAAACCTATCCTTGGAAAGAATACAAGGAAATTTATACCCCTGAAGTTGTTGAGGCCGGCGACATGATGCTCTATGGCTG
GATTGGTGACAATGGTGACCCGGATAACTTCTTATCTTTGCTGGAAACCAAGGAAATCAAGAGCACTCTGAATGCTGCCA
AGTACTCCAACAAGCAGGTAGATGAACTGCTAATGAAAGGTCGTACCGCCCGAACTCAGGAAGAGCGGAACGCTGCTTAC
AGTGAATTGCAAAAAATTGTACAGGCCGAAGCTCCCTGGGTGTTCATTAGCCACAGTAAAGATATGGCTGCTGTAAGCAA
GAAAGTAGAAGGCTTTGATCTACACCCCGTAGGTGTTGTATACTTCCGTGATGTAGATATCAAGTAG

Upstream 100 bases:

>100_bases
TTGTAGAAAATTTAACCACTAGTCCCAGTGAAGACAGCTGTAAGATAAGGATAGACACTGGGGAGATTCTAAACAAATAA
GGAGAGGAGTTGCCAACTAA

Downstream 100 bases:

>100_bases
TAAATCAATCACCTTTGGAAGAAACACGAATATGGCCCATGCCATATTCGTGTTTCAATGTTTTACGGTCAATAGGAAGA
ATTTAGTATTTTTATTACCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MFRKKSFFMLLLVSLLAVAMVGCGGGDNKEKAADKAKQKVFVFGQGSDPRGLDPAFVDDGESANPIVNIYDGLVRYKTGS
TEIEPALATEWSSSEDGKEWTFKLRQGVKFHDGTPFNADAVVFSVNRQLPPNRTDAMPYASFTFGPVEKIEKVDDYTVKF
ILNEPYAPFLANLAMALAAPIVSPAAVEKYGDKFIENPVGTGPFKFVEWKKGQQIVLEANKEYWGGAPKLDKLVYKFIKE
NSVRASELRTGSIQAMNGVDPNDVKMLEEAGLNVIKNPGMNINYLAFFCNKKPFDNPKLRLAVAHAINRENLINFLYQGL
AELPNSMLPNFMPGHDKSLQAPEYNPEKAKQLLAEAGYPNGLKVTLLTYSTVRPYNPVGGDKLAAAVQADLRKVGIEAEI
KTYPWKEYKEIYTPEVVEAGDMMLYGWIGDNGDPDNFLSLLETKEIKSTLNAAKYSNKQVDELLMKGRTARTQEERNAAY
SELQKIVQAEAPWVFISHSKDMAAVSKKVEGFDLHPVGVVYFRDVDIK

Sequences:

>Translated_528_residues
MFRKKSFFMLLLVSLLAVAMVGCGGGDNKEKAADKAKQKVFVFGQGSDPRGLDPAFVDDGESANPIVNIYDGLVRYKTGS
TEIEPALATEWSSSEDGKEWTFKLRQGVKFHDGTPFNADAVVFSVNRQLPPNRTDAMPYASFTFGPVEKIEKVDDYTVKF
ILNEPYAPFLANLAMALAAPIVSPAAVEKYGDKFIENPVGTGPFKFVEWKKGQQIVLEANKEYWGGAPKLDKLVYKFIKE
NSVRASELRTGSIQAMNGVDPNDVKMLEEAGLNVIKNPGMNINYLAFFCNKKPFDNPKLRLAVAHAINRENLINFLYQGL
AELPNSMLPNFMPGHDKSLQAPEYNPEKAKQLLAEAGYPNGLKVTLLTYSTVRPYNPVGGDKLAAAVQADLRKVGIEAEI
KTYPWKEYKEIYTPEVVEAGDMMLYGWIGDNGDPDNFLSLLETKEIKSTLNAAKYSNKQVDELLMKGRTARTQEERNAAY
SELQKIVQAEAPWVFISHSKDMAAVSKKVEGFDLHPVGVVYFRDVDIK
>Mature_528_residues
MFRKKSFFMLLLVSLLAVAMVGCGGGDNKEKAADKAKQKVFVFGQGSDPRGLDPAFVDDGESANPIVNIYDGLVRYKTGS
TEIEPALATEWSSSEDGKEWTFKLRQGVKFHDGTPFNADAVVFSVNRQLPPNRTDAMPYASFTFGPVEKIEKVDDYTVKF
ILNEPYAPFLANLAMALAAPIVSPAAVEKYGDKFIENPVGTGPFKFVEWKKGQQIVLEANKEYWGGAPKLDKLVYKFIKE
NSVRASELRTGSIQAMNGVDPNDVKMLEEAGLNVIKNPGMNINYLAFFCNKKPFDNPKLRLAVAHAINRENLINFLYQGL
AELPNSMLPNFMPGHDKSLQAPEYNPEKAKQLLAEAGYPNGLKVTLLTYSTVRPYNPVGGDKLAAAVQADLRKVGIEAEI
KTYPWKEYKEIYTPEVVEAGDMMLYGWIGDNGDPDNFLSLLETKEIKSTLNAAKYSNKQVDELLMKGRTARTQEERNAAY
SELQKIVQAEAPWVFISHSKDMAAVSKKVEGFDLHPVGVVYFRDVDIK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=551, Percent_Identity=35.3901996370236, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI1787052, Length=496, Percent_Identity=32.258064516129, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1787762, Length=537, Percent_Identity=30.5400372439479, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787551, Length=538, Percent_Identity=29.182156133829, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1787495, Length=552, Percent_Identity=25.5434782608696, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1789397, Length=554, Percent_Identity=26.8953068592058, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789887, Length=460, Percent_Identity=26.9565217391304, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081878, Length=523, Percent_Identity=27.3422562141491, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58718; Mature: 58718

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKKSFFMLLLVSLLAVAMVGCGGGDNKEKAADKAKQKVFVFGQGSDPRGLDPAFVDDG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCC
ESANPIVNIYDGLVRYKTGSTEIEPALATEWSSSEDGKEWTFKLRQGVKFHDGTPFNADA
CCCCCEEEECCCCEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCE
VVFSVNRQLPPNRTDAMPYASFTFGPVEKIEKVDDYTVKFILNEPYAPFLANLAMALAAP
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
IVSPAAVEKYGDKFIENPVGTGPFKFVEWKKGQQIVLEANKEYWGGAPKLDKLVYKFIKE
HCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECHHHCCCCCHHHHHHHHHHHC
NSVRASELRTGSIQAMNGVDPNDVKMLEEAGLNVIKNPGMNINYLAFFCNKKPFDNPKLR
CCCCHHHHCCCCEEEECCCCHHHHHHHHHCCCCEECCCCCCEEEEEEEECCCCCCCCCEE
LAVAHAINRENLINFLYQGLAELPNSMLPNFMPGHDKSLQAPEYNPEKAKQLLAEAGYPN
EEHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
GLKVTLLTYSTVRPYNPVGGDKLAAAVQADLRKVGIEAEIKTYPWKEYKEIYTPEVVEAG
CCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCCCCC
DMMLYGWIGDNGDPDNFLSLLETKEIKSTLNAAKYSNKQVDELLMKGRTARTQEERNAAY
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHH
SELQKIVQAEAPWVFISHSKDMAAVSKKVEGFDLHPVGVVYFRDVDIK
HHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MFRKKSFFMLLLVSLLAVAMVGCGGGDNKEKAADKAKQKVFVFGQGSDPRGLDPAFVDDG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCC
ESANPIVNIYDGLVRYKTGSTEIEPALATEWSSSEDGKEWTFKLRQGVKFHDGTPFNADA
CCCCCEEEECCCCEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCE
VVFSVNRQLPPNRTDAMPYASFTFGPVEKIEKVDDYTVKFILNEPYAPFLANLAMALAAP
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
IVSPAAVEKYGDKFIENPVGTGPFKFVEWKKGQQIVLEANKEYWGGAPKLDKLVYKFIKE
HCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECHHHCCCCCHHHHHHHHHHHC
NSVRASELRTGSIQAMNGVDPNDVKMLEEAGLNVIKNPGMNINYLAFFCNKKPFDNPKLR
CCCCHHHHCCCCEEEECCCCHHHHHHHHHCCCCEECCCCCCEEEEEEEECCCCCCCCCEE
LAVAHAINRENLINFLYQGLAELPNSMLPNFMPGHDKSLQAPEYNPEKAKQLLAEAGYPN
EEHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
GLKVTLLTYSTVRPYNPVGGDKLAAAVQADLRKVGIEAEIKTYPWKEYKEIYTPEVVEAG
CCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCCCCC
DMMLYGWIGDNGDPDNFLSLLETKEIKSTLNAAKYSNKQVDELLMKGRTARTQEERNAAY
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHH
SELQKIVQAEAPWVFISHSKDMAAVSKKVEGFDLHPVGVVYFRDVDIK
HHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]