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The map label for this gene is ppaX [H]

Identifier: 134300288

GI number: 134300288

Start: 2664192

End: 2664845

Strand: Reverse

Name: ppaX [H]

Synonym: Dred_2449

Alternate gene names: 134300288

Gene position: 2664845-2664192 (Counterclockwise)

Preceding gene: 134300289

Following gene: 134300287

Centisome position: 73.86

GC content: 46.02

Gene sequence:

>654_bases
ATGATAAAAACAATACTATTTGATTTGGATGGCACACTCCTGGATTCACTGCCTTTAATTAAGAGGACCTATAAGAGGGT
CTTTCAAGAAATGAATATACCCTGGGCTAACGGCGAGGTCATGAAATGTATTGGATTGCCGCTGGTTGATATCGGTAAGA
AGTTTGCCGGAGAGGAAAGGCACGCAGAATTTTTTAGTTTATACCAGCAGCATTATGCCATCGAGCACGATGCCATGACA
AAAGCCTACCCCGGTACCATGGAAATGCTAGAAGACCTGCACCAAAGAAGTCTTCGCCTGGGCGTGGTTACTTCCAAATC
CCGCCGAGTTGCATTGAGAAGCACAGGGTTTTTAGGAATTGATCGCTACATGGATGTTCTGATCGGTGTAGAGGATGTGG
ACCGTCATAAGCCTCAGCCAGACCCTATTTTTAAAGCCCTGGAACAAATGCAAGTGCCTGCAGAAGGTGCCGCTTACATC
GGGGATAGCCCCTTTGACATTATGTCTGCCAAAGCTGCCGGCGTAACTTCCATCGGCGTTAGCTGGGGAATGGCAGAGGG
TGATGAACTCCTGCGCTTTGAACCGGATTATCTTCTAAACCAATGGTCAGATTTATTGTTAGTACTGGAAAATGATACCG
AATTAATGGACTAA

Upstream 100 bases:

>100_bases
TTTTAGCCGTCAGCCGTAGGCTTGAGGGATAGCTGATGGCATTTCTTGGCTTTTGTGCTTTTTCGTAGTAAAATACCAGG
ATAAAGAACGGAGGGGTCTC

Downstream 100 bases:

>100_bases
TGAAAAGATCTTTTCATAGTTCCTAATAATGGCCAAAAGTCACCAAGGGGATGAAAAAAACATAACCTATGATAAGCGCG
GAAAGTAGGTGTGAGTCATG

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MIKTILFDLDGTLLDSLPLIKRTYKRVFQEMNIPWANGEVMKCIGLPLVDIGKKFAGEERHAEFFSLYQQHYAIEHDAMT
KAYPGTMEMLEDLHQRSLRLGVVTSKSRRVALRSTGFLGIDRYMDVLIGVEDVDRHKPQPDPIFKALEQMQVPAEGAAYI
GDSPFDIMSAKAAGVTSIGVSWGMAEGDELLRFEPDYLLNQWSDLLLVLENDTELMD

Sequences:

>Translated_217_residues
MIKTILFDLDGTLLDSLPLIKRTYKRVFQEMNIPWANGEVMKCIGLPLVDIGKKFAGEERHAEFFSLYQQHYAIEHDAMT
KAYPGTMEMLEDLHQRSLRLGVVTSKSRRVALRSTGFLGIDRYMDVLIGVEDVDRHKPQPDPIFKALEQMQVPAEGAAYI
GDSPFDIMSAKAAGVTSIGVSWGMAEGDELLRFEPDYLLNQWSDLLLVLENDTELMD
>Mature_217_residues
MIKTILFDLDGTLLDSLPLIKRTYKRVFQEMNIPWANGEVMKCIGLPLVDIGKKFAGEERHAEFFSLYQQHYAIEHDAMT
KAYPGTMEMLEDLHQRSLRLGVVTSKSRRVALRSTGFLGIDRYMDVLIGVEDVDRHKPQPDPIFKALEQMQVPAEGAAYI
GDSPFDIMSAKAAGVTSIGVSWGMAEGDELLRFEPDYLLNQWSDLLLVLENDTELMD

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=235, Percent_Identity=29.3617021276596, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI87082080, Length=184, Percent_Identity=28.804347826087, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 24543; Mature: 24543

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTILFDLDGTLLDSLPLIKRTYKRVFQEMNIPWANGEVMKCIGLPLVDIGKKFAGEER
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCCHHHHHHHHCCCHH
HAEFFSLYQQHYAIEHDAMTKAYPGTMEMLEDLHQRSLRLGVVTSKSRRVALRSTGFLGI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCCHH
DRYMDVLIGVEDVDRHKPQPDPIFKALEQMQVPAEGAAYIGDSPFDIMSAKAAGVTSIGV
HHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEECCC
SWGMAEGDELLRFEPDYLLNQWSDLLLVLENDTELMD
CCCCCCCCHHEEECHHHHHHHHHHEEEEEECCHHCCC
>Mature Secondary Structure
MIKTILFDLDGTLLDSLPLIKRTYKRVFQEMNIPWANGEVMKCIGLPLVDIGKKFAGEER
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCCHHHHHHHHCCCHH
HAEFFSLYQQHYAIEHDAMTKAYPGTMEMLEDLHQRSLRLGVVTSKSRRVALRSTGFLGI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCCHH
DRYMDVLIGVEDVDRHKPQPDPIFKALEQMQVPAEGAAYIGDSPFDIMSAKAAGVTSIGV
HHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEECCC
SWGMAEGDELLRFEPDYLLNQWSDLLLVLENDTELMD
CCCCCCCCHHEEECHHHHHHHHHHEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]